A New Method for Generation of Soundings from Phase-Difference Measurements

A desirable feature of bathymetric sonar systems is the production of statistically independent soundings allowing a system to achieve its full capability in resolution and object detection. Moreover gridding algorithms such as the Combined Uncertainty Bathymetric Estimator (CUBE) rely on the statistical independence of soundings to properly estimate depth and discriminate outliers. Common methods of filtering to mitigate uncertainty in the signal processing of both multibeam and phase-differencing sidescan systems (curve fitting in zero-crossing detections and differential phase filtering respectively) can produce correlated soundings. Here we propose an alternative method for the generation of soundings from differential phase measurements made by either sonar type to produce statistically independent soundings. The method extracts individual, non-overlapping and unfiltered, phase-difference measurements (from either sonar type) converting these to sonar-relative receive angle, estimates their uncertainty, fixes the desired depth uncertainty level and combines these individual measurements into an uncertainty-weighted mean to achieve the desired depth uncertainty, and no more. When the signal to noise ratio is sufficiently high such that the desired depth uncertainty is achieved with an individual measurement, bathymetric estimates are produced at the sonar’s full resolution capability. When multiple measurements are required, the filtering automatically adjusts to maintain the desired uncertainty level, degrading the resolution only as necessary. Because no two measurements contribute to a single reported sounding, the resulting estimated soundings are statistically independent and therefore better resolve adjacent objects, increase object detectability and are more suitable for statistical gridding methodologies

Optimizing Resolution and Uncertainty in Bathymetric Sonar Systems

Bathymetric sonar systems (whether multibeam or phase-differencing sidescan) contain an inherent trade-off between resolution and uncertainty. Systems are traditionally designed with a fixed spatial resolution, and the parameter settings are optimized to minimize the uncertainty in the soundings within that constraint. By fixing the spatial resolution of the system, current generation sonars operate sub-optimally when the SNR is high, producing soundings with lower resolution than is supportable by the data, and inefficiently when the SNR is low, producing high-uncertainty soundings of little value. Here we propose fixing the sounding measurement uncertainty instead, and optimizing the resolution of the system within that uncertainty constraint. Fixing the sounding measurement uncertainty produces a swath with a variable number of bathymetric estimates per ping, in which each estimate’s spatial resolution is optimized by combining measurements only until the desired depth uncertainty is achieved. When the signal to noise ratio is sufficiently high such that the desired depth uncertainty is achieved with individual measurements, bathymetric estimates are produced at the sonar’s full resolution capability. Correspondingly, a sonar’s resolution is no-longer only considered as a property of the sonar (based on, for example, beamwidth and bandwidth,) but now incorporates geometrical aspects of the measurements and environmental factors (e.g., seafloor scattering strength). Examples are shown from both multibeam and phase- differencing sonar systems

Direct Assessment of Juvenile Atlantic Bluefin Tuna: Integrating Sonar and Aerial Results in Support of Fishery-Incident Surveys

There is a clear need for direct assessment approaches for Atlantic bluefin tuna (Thunnus thynnus, ABFT), including formulation of experimental designs and pilot surveys for abundance estimation. In the western Atlantic, aerial surveys are highly feasible for juvenile ABFT because of their surface availability in summer and autumn on the mid-Atlantic shelf. Our goals are to design, implement, and analyze a fisheries-independent survey of juvenile ABFT and to assess the feasibility of biomass estimation in the Gulf of Maine (USA). From initial field trials using sonar and aerial mapping we demonstrated feasibility of determining size, area, and total biomass of schools as well as sizes of individuals within schools. We used aerial imagery to determine the school’s surface shape and to enumerate bluefin tuna visible in the upper few meters of the water column. The sonar data provided information on school height and number of individuals not captured in aerial photographs. By integrating sonar and aerial data we can estimate school biomass, number and sizes of individuals in schools, and aggregation behavior. In 2015 we plan to use a marine hexacopter to obtain more highly resolved aerial images of schools, with improved geo-rectification required for automated target recognition and objective counts of individuals. Current bluefin surveys rely primarily on observer and spotter pilot estimates of school metrics. Although not without challenges, the analytical techniques we’re developing will provide more objective, multi-dimensional information on ABFT schools and less biased estimates of biomass. Direct assessment also offers a means of tracking shifts in coastal distribution of highly mobile ABFT, especially as traditional indices of abundance may no longer be appropriate

Interaction Between Visual and Phonotactic Orientation During Flight in \u3ci\u3eMagicicada Cassini\u3c/i\u3e (Homoptera: Cicadidae)

Visual and phonotactic orientation often occur simultaneously in diurnal cicadas. and these animals generally have their largest sensory elaboration in eyes and hearing organs. Phonotactic orientation occurs principally during flight. Males and females of Magicicada cassini commonly perform low- altitude « 5 m) and short-distance « 15 m) flights in their natural habitat at flight speeds of 3 to 6 m/s. During flight, the long body axis is tilted 10° to Q , head upward. Wing beat frequencies of tethered animals at 24° to 26°C averaged 28.8 Hz. Body temperature in the field for flying individuals aver- aged 4.6°C above ambient. Compound eyes of females possess about 7% more facets than males, and the binocular field of view for both is especially expanded dorsa-frontally, frontally, and fronto-ventrally. The role of vision for phonoresponses, and in flight and landing behavior. was studied in nature by comparing controls with cicadas with eyes partly to completely covered with aluminum paint. Cicadas with their three ocelli covered behaved like controls and exhibited low-altitude and short-distance flights with landings on neighboring shrubs, as did cicadas with only both caudal halves or both dorsal halves of the compound eyes covered. Those with both compound eyes covered completely (with or without additionally covering the three ocelli) flew to higher altitudes and for longer distances. Higher and longer flight courses were also seen in cicadas (A) with only one compound eye covered. which in addition circled during walking and flight toward the side of unrestricted vision, (B) with both frontal or both ventral halves of their compound eyes covered. and (C) with either the binocular or monocular fields of the eyes covered. Thus, the paired fronta-antero-ventral regions of the compound eyes provide visual information for habitat-dependent low-altitude flights and landings. Females with intact compound eyes and ocelli responded to playbacks of just the frequency/intensity sweep at the end of the buzz in calling songs of a male by flying within 1.2 m above the ground and landing on a nylon screen- covered small bush directly above the loudspeaker from distances of 2 to 8 m. mostly from lower vegetation. Males that were blinded, or blinded and deafened, sang less and flew less than normal males. However. they performed all of those behaviors, and all also walked and fed. Periodical cicadas (Magicicada, Tibicininae) are known for synchronized adult emergence and noisy aggregations of millions of individuals of three intermingled species in each brood population (Alexander and Moore 1962). Broods are isolated geographically and chronologically, such that in some years no periodical cicada adults emerge, and most areas of the eastern United States have only one brood population appearing as adults at intervals of either 13 or 17 years. In all Magicicada species, daily flights affect spacing and aggregation of both sexes during feeding, chorusing, mating, and ovipositing. Flights are mediated by both acoustical and visual cues. Each species in these aggregations establishes mating leks. These aggregations continue to mix, every day and unpredictably, during the emergence period. Toward the end of the reproductive season, males die sooner than females, leading to little or no chorusing, and then females disperse progressively further from the lek sites. The cohesive effect of the acoustical cues of chorusing males on these cicada populations is obvious. Both sexes of all six species of periodical cicadas live and feed on shrubs and trees of different species, sizes, and shapes, and females lay eggs in their living twigs. Their niches overlap almost completely, the three species of 13­ year or 17-year cicadas being separated principally by diurnal acoustic behavior leading to aggregation sites that change every day and are seldom exclusive to a single Magicicada species. Adults frequently change location in these complex visual environments by short-distance and low-altitude flights. which we call bush-hopping. These flights are associated with sound communication and reproductive activities and are most commonly observed during bright sunlight and at ambient temperatures above 25°C with little wind (Alexander and Moore 1958,1962; Dunning et al. 1979). Otte (1990) and Toms (1992) discuss the common correlation between hearing and flying in orthop­teroid insects, interactions basically similar to those found in cicadas. The present paper describes the interaction of vision (compound eyes and ocelli) and phonoresponses of males and females of Magicicada cassini (Fisher) in walking, but especially in flight and landing behavior, within a natural habitat

Impaired osteoblast differentiation in annexin A2- and -A5-deficient cells.

Annexins are a class of calcium-binding proteins with diverse functions in the regulation of lipid rafts, inflammation, fibrinolysis, transcriptional programming and ion transport. Within bone, they are well-characterized as components of mineralizing matrix vesicles, although little else is known as to their function during osteogenesis. We employed shRNA to generate annexin A2 (AnxA2)- or annexin A5 (AnxA5)-knockdown pre-osteoblasts, and determined whether proliferation or osteogenic differentiation was altered in knockdown cells, compared to pSiren (Si) controls. We report that DNA content, a marker of proliferation, was significantly reduced in both AnxA2 and AnxA5 knockdown cells. Alkaline phosphatase expression and activity were also suppressed in AnxA2- or AnxA5-knockdown after 14 days of culture. The pattern of osteogenic gene expression was altered in knockdown cells, with Col1a1 expressed more rapidly in knock-down cells, compared to pSiren. In contrast, Runx2, Ibsp, and Bglap all revealed decreased expression after 14 days of culture. In both AnxA2- and AnxA5-knockdown, interleukin-induced STAT6 signaling was markedly attenuated compared to pSiren controls. These data suggest that AnxA2 and AnxA5 can influence bone formation via regulation of osteoprogenitor proliferation, differentiation, and responsiveness to cytokines in addition to their well-studied function in matrix vesicles

Acoustic positioning and tracking in Portsmouth Harbour, New Hampshire

Portsmouth Harbor, New Hampshire, is frequently used as a testing area for multibeam and sidescan sonars, and is the location of numerous ground-truthing studies. Having the ability to accurately position underwater sensors is an important aspect of this type of work. However, underwater positioning in Portsmouth Harbor is challenging. It is relatively shallow, approximately one kilometer wide with depths of less than 25 meters. There is mixing between fresh river water and seawater, which is intensified by high currents and strong tides. This causes a very complicated spatial and temporal sound speed structure. Solutions that use the time-of-arrival of an acoustic pulse to estimate range will require very precise knowledge of the travel paths of the signal in order to separate out issues of multipath arrivals. An alternative solution is to use the phase measurements between closely spaced hydrophones to measure the bearing of an acoustic pinger. By using two bearing measurement devices that are widely separated, the intersection of the two bearings can be used to position the pinger. The advantage of this approach is that the sound speed only needs to be known at the location of the phase measurements. Both time-of-arrival and phase difference systems may encounter difficulties arising from horizontal refraction due to spatially varying sound speed. To ascertain which solution would be optimal in Portsmouth Harbor, the time-of-arrival and phase measurement approaches are being examined individually. Initial field tests have been conducted using a 40 kHz signal to look at bearing accuracy. Using hydrophones that are spaced 2/3 wavelengths apart, the bearing accuracy was found to be 1.25deg for angles up to 20deg from broadside with signal to noise ratios (SNR) greater than 15 dB. The results from the closely spaced hydrophones were used to resolve phase ambiguities, allowing finer bearing measurements to be made between hydrophones spaced 5 wavelengths apart. The fi- ne bearing measurements resulted in a bearing accuracy of 0.3deg for angles up to 20deg from broadside with SNR greater than 15 dB. Field tests planned for summer 2007 will include a more detailed investigation of how the environmental influences affect each of the measurement types including range, signal to noise ratio, currents, and sound speed structure

Isoprenoids determine Th1/Th2 fate in pathogenic T cells, providing a mechanism of modulation of autoimmunity by atorvastatin.

3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is a critical enzyme in the mevalonate pathway that regulates the biosynthesis of cholesterol as well as isoprenoids that mediate the membrane association of certain GTPases. Blockade of this enzyme by atorvastatin (AT) inhibits the destructive proinflammatory T helper cell (Th)1 response during experimental autoimmune encephalomyelitis and may be beneficial in the treatment of multiple sclerosis and other Th1-mediated autoimmune diseases. Here we present evidence linking specific isoprenoid intermediates of the mevalonate pathway to signaling pathways that regulate T cell autoimmunity. We demonstrate that the isoprenoid geranylgeranyl-pyrophosphate (GGPP) mediates proliferation, whereas both GGPP and its precursor, farnesyl-PP, regulate the Th1 differentiation of myelin-reactive T cells. Depletion of these isoprenoid intermediates in vivo via oral AT administration hindered these T cell responses by decreasing geranylgeranylated RhoA and farnesylated Ras at the plasma membrane. This was associated with reduced extracellular signal-regulated kinase (ERK) and p38 phosphorylation and DNA binding of their cotarget c-fos in response to T cell receptor activation. Inhibition of ERK and p38 mimicked the effects of AT and induced a Th2 cytokine shift. Thus, by connecting isoprenoid availability to regulation of Th1/Th2 fate, we have elucidated a mechanism by which AT may suppress Th1-mediated central nervous system autoimmune disease

Integrable and Nonintegrable Classical Spin Clusters: Integrability Criteria and Analytic Structure of Invariants

The nonlinear dynamics is investigated for a system of N classical spins. This represents a Hamiltonian system with N degrees of freedom. According to the Liouville theorem, the complete integrability of such a system requires the existence of N independent integrals of the motion which are mutually in involution. As a basis for the investigation of regular and chaotic spin motions, we have examined in detail the problem of integrability of a two-spin system. It represents the simplest autonomous spin system for which the integrability problem is nontrivial. We have shown that a pair of spins coupled by an anisotropic exchange interaction represents a completely integrable system for any values of the coupling constants. The second integral of the motion (in addition to the Hamiltonian), which ensures the complete integrability, turns out to be quadratic in the spin variables. If, in addition to the exchange anisotropy also single-site anisotropy terms are included in the two-spin Hamiltonian, a second integral of the motion quadratic in the spin variables exists and thus guarantees integrability, only if the model constants satisfy a certain condition. Our numerical calculations strongly suggest that the violation of this condition implies not only the nonexistence of a quadratic integral, but the nonexistence of a second independent integral of motion in general. Finally, as an example of a completely integrable N-spin system we present the Kittel-Shore model of uniformly interacting spins, for which we have constructed the N independent integrals in involution as well as the action-angle variables explicitly