Cholecystokinin and the ontogeny of digestion in the red drum (Sciaenops ocellatus)

textWhile substantial progress has been made in replacing live prey with artificial diets in the feeding of marine fish larvae, it still remains impossible to successfully rear larvae on artificial diets without some period of co-feeding live prey or algae. This study investigated the presence and role of the gastrointestinal hormone cholecystokinin (CCK) in the red drum (Sciaenops ocellatus) to gain a better understanding of the factors limiting the utilization of artificial diets by red drum larvae. Work with other fish species has shown that CCK is the principal hormone which regulates the release of pancreatic enzymes into the gut lumen and emphasizes the potential importance of CCK in early red drum larvae. This work investigated the hypothesis that some signal present in the live prey or algae stimulates CCK and thereby initiates the digestive process in the larvae. First, the nucleotide and amino acid sequence of the putative red drum cholecystokinin precursor was determined and the development of CCK immunoreactive cells was examined. This work showed that red drum CCK is highly similar to CCK in other vertebrates and can be detected in the digestive tract of larval red drum within three days after the initiation of exogenous feeding. Next; postprandial trypsin, CCK, and CCK mRNA responses were quantified in red drum juveniles and larvae over a three hour period. Both CCK and trypsin were increased within thirty minutes following feeding while CCK mRNA levels were increased within the next two to three hours. Finally, the trypsin, CCK, and CCK mRNA responses of red drum larvae to homogenates of live prey and algae were examined. Homogenized rotifers appeared to be sufficient to induce both the CCK and trypsin responses in larval red drum. These results suggest that in addition to other factors, some component of live prey may initiate the release of CCK and prime the digestive process. Understanding these factors and their effects in early larvae may allow us to formulate and produce a prepared diet which will support growth and survival to metamorphosis equal to that provided by live feeds.Marine Scienc

Lucas' Theorem for Prime Powers

Lucas' theorem on binomial coefficients states that (AB)≡(arbr)⋯(a1b1)(a0b0)(mod p) where p is a prime and A = arpr + ⋯ + a0p + a0, B = brpr + ⋯ + b1p + b0 + are the p-adic expansions of A and B. If s ⩾ 2, it is shown that a similar formula holds modulo ps where the product involves a slightly modified binomial coefficient evaluated on blocks of s digits.

Final technical report for NSF grant #OCE 75-20241, entitled Identification and role of the ultraplankton of the lower Chesapeake Bay region

Numbers and species identities of the most dominant small (\u3c15μm) photosynthetic Plankton were determined from samples of lower Chesapeake Bay water. The data is being compared to physical and chemical measurements of the Bay waters in an effort to determine conditions which encourage or inhibit growth of the organisms. Pure cultures of some of the more common species have been established and are being used to evaluate the roles of the organisms in terms of photosynthetic rates and utilization and excretion of nitrogen compounds