545 research outputs found
Catastrophic Fermi surface reconstruction in the shape-memory alloy AuZn
AuZn undergoes a shape-memory transition at 67 K. The de Haas van Alphen
effect persists to 100 K enabling the observation of a change in the quantum
oscillation spectrum indicative of a catastrophic Fermi surface reconstruction
at the transition. Coexistence of both Fermi surfaces at low temperatures is
suggestive of an intrinsic phase separation in the bulk of the material. In
addition, a Dingle analysis reveals a sharp change in the scattering mechanism
at a threshold cyclotron radius, which we suggest to be related to the
underlying microstructure that drives the shape-memory effect.Comment: 4 pages, 4 figure
Size of Orbital Ordering Domain Controlled by the Itinerancy of the 3d Electrons in a Manganite Thin Film
An electronic effect on a macroscopic domain structure is found in a strongly
correlated half-doped manganite film NdSrMnO3 grown on a (011)
surface of SrTiO3. The sample has a high-temperature (HT) phase free from
distortion above 180K and two low-temperature (LT) phases with a large
shear-mode strain and a concomitant twin structure. One LT phase has a large
itinerancy (A-type), and the other has a small itinerancy (CE-type), while the
lattice distortions they cause are almost equal. Our x ray diffraction
measurement shows that the domain size of the LT phase made by the HT-CE
transition is much smaller than that by the HT-A transition, indicating that
the difference in domain size is caused by the electronic states of the LT
phases.Comment: 9 pages, 4 figure
Zebrafish Reproduction: Revisiting In Vitro Fertilization to Increase Sperm Cryopreservation Success
Although conventional cryopreservation is a proven method for long-term, safe storage of genetic material, protocols used by the zebrafish community are not standardized and yield inconsistent results, thereby putting the security of many genotypes in individual laboratories and stock centers at risk. An important challenge for a successful zebrafish sperm cryopreservation program is the large variability in the post-thaw in vitro fertilization success (0 to 80%). But how much of this variability was due to the reproductive traits of the in vitro fertilization process, and not due to the cryopreservation process? These experiments only assessed the in vitro process with fresh sperm, but yielded the basic metrics needed for successful in vitro fertilization using cryopreserved sperm, as well. We analyzed the reproductive traits for zebrafish males with a strict body condition range. It did not correlate with sperm volume, or motility (P>0.05), but it did correlate with sperm concentration. Younger males produced more concentrated sperm (P<0.05). To minimize the wastage of sperm during the in vitro fertilization process, 106 cells/ml was the minimum sperm concentration needed to achieve an in vitro fertilization success of ≥ 70%. During the in vitro process, pooling sperm did not reduce fertilization success (P>0.05), but pooling eggs reduced it by approximately 30 to 50% (P<0.05). This reduction in fertilization success was due not to the pooling of the females' eggs, but to the type of tools used to handle the eggs. Recommendations to enhance the in vitro process for zebrafish include: 1) using males of a body condition closer to 1.5 for maximal sperm concentration; 2) minimizing sperm wastage by using a working sperm concentration of 106 motile cells/ml for in vitro fertilization; and 3) never using metal or sharp-edged tools to handle eggs prior to fertilization
Fermi Surface as a Driver for the Shape-Memory Effect in AuZn
Martensites are materials that undergo diffusionless, solid-state
transitions. The martensitic transition yields properties that depend on the
history of the material and may allow it to recover its previous shape after
plastic deformation. This is known as the shape-memory effect (SME). We have
succeeded in identifying the primary electronic mechanism responsible for the
martensitic transition in the shape-memory alloy AuZn by using Fermi-surface
measurements (de Haas-van Alphen oscillations) and band-structure calculations.
This strongly suggests that electronic band structure is an important
consideration in the design of future SME alloys
Crystallographic structure of ultrathin Fe films on Cu(100)
We report bcc-like crystal structures in 2-4 ML Fe films grown on fcc Cu(100)
using scanning tunneling microscopy. The local bcc structure provides a
straightforward explanation for their frequently reported outstanding magnetic
properties, i.e., ferromagnetic ordering in all layers with a Curie temperature
above 300 K. The non-pseudomorphic structure, which becomes pseudomorphic above
4 ML film thickness is unexpected in terms of conventional rules of thin film
growth and stresses the importance of finite thickness effects in ferromagnetic
ultrathin films.Comment: 4 pages, 3 figures, RevTeX/LaTeX2.0
"Cold Melting" of Invar Alloys
An anomalously strong volume magnetostriction in Invars may lead to a
situation when at low temperatures the dislocation free energy becomes negative
and a multiple generation of dislocations becomes possible. This generation
induces a first order phase transition from the FCC crystalline to an amorphous
state, and may be called "cold melting". The possibility of the cold melting in
Invars is connected with the fact that the exchange energy contribution into
the dislocation self energy in Invars is strongly enhanced, as compared to
conventional ferromagnetics, due to anomalously strong volume magnetostriction.
The possible candidate, where this effect can be observed, is a FePt disordered
Invar alloy in which the volume magnetostriction is especially large
Does type 2 diabetes influence the risk of oesophageal adenocarcinoma?
Since hyperinsulinaemia may promote obesity-linked cancers, we compared type 2 diabetes prevalence among oesophageal adenocarcinoma (OAC) patients and population controls. Diabetes increased the risk of OAC (adjusted odds ratio 1.59, 95% confidence interval (CI) 1.04–2.43), although the risk was attenuated after further adjusting for body mass index (1.32, 95% CI 0.85–2.05)
Active Site Mutations Change the Cleavage Specificity of Neprilysin
Neprilysin (NEP), a member of the M13 subgroup of the zinc-dependent endopeptidase family is a membrane bound peptidase capable of cleaving a variety of physiological peptides. We have generated a series of neprilysin variants containing mutations at either one of two active site residues, Phe563 and Ser546. Among the mutants studied in detail we observed changes in their activity towards leucine5-enkephalin, insulin B chain, and amyloid β1–40. For example, NEPF563I displayed an increase in preference towards cleaving leucine5-enkephalin relative to insulin B chain, while mutant NEPS546E was less discriminating than neprilysin. Mutants NEPF563L and NEPS546E exhibit different cleavage site preferences than neprilysin with insulin B chain and amyloid ß1–40 as substrates. These data indicate that it is possible to alter the cleavage site specificity of neprilysin opening the way for the development of substrate specific or substrate exclusive forms of the enzyme with enhanced therapeutic potential
Utilisation of an operative difficulty grading scale for laparoscopic cholecystectomy
Background
A reliable system for grading operative difficulty of laparoscopic cholecystectomy would standardise description of findings and reporting of outcomes. The aim of this study was to validate a difficulty grading system (Nassar scale), testing its applicability and consistency in two large prospective datasets.
Methods
Patient and disease-related variables and 30-day outcomes were identified in two prospective cholecystectomy databases: the multi-centre prospective cohort of 8820 patients from the recent CholeS Study and the single-surgeon series containing 4089 patients. Operative data and patient outcomes were correlated with Nassar operative difficultly scale, using Kendall’s tau for dichotomous variables, or Jonckheere–Terpstra tests for continuous variables. A ROC curve analysis was performed, to quantify the predictive accuracy of the scale for each outcome, with continuous outcomes dichotomised, prior to analysis.
Results
A higher operative difficulty grade was consistently associated with worse outcomes for the patients in both the reference and CholeS cohorts. The median length of stay increased from 0 to 4 days, and the 30-day complication rate from 7.6 to 24.4% as the difficulty grade increased from 1 to 4/5 (both p < 0.001). In the CholeS cohort, a higher difficulty grade was found to be most strongly associated with conversion to open and 30-day mortality (AUROC = 0.903, 0.822, respectively). On multivariable analysis, the Nassar operative difficultly scale was found to be a significant independent predictor of operative duration, conversion to open surgery, 30-day complications and 30-day reintervention (all p < 0.001).
Conclusion
We have shown that an operative difficulty scale can standardise the description of operative findings by multiple grades of surgeons to facilitate audit, training assessment and research. It provides a tool for reporting operative findings, disease severity and technical difficulty and can be utilised in future research to reliably compare outcomes according to case mix and intra-operative difficulty
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