Physical activity, sitting and depression during pregnancy

Aims The aim of this cross sectional study is to explore levels of physical activity and sitting behaviour amongst a sample of pregnant Australian women (n = 81), and investigate whether reported levels of physical activity and/or time spent sitting were associated with depressive symptom scores after controlling for potential covariates. Methods Study participants were women who attended the antenatal clinic of a large Brisbane maternity hospital between October and November 2006. Data relating to participants. current levels of physical activity, sitting behaviour, depressive symptoms, demographic characteristics and exposure to known risk factors for depression during pregnancy were collected; via on-site survey, follow-up telephone interview (approximately one week later) and post delivery access to participant hospital records. Results Participants were aged 29.5 (¡¾ 5.6) years and mostly partnered (86.4%) with a gross household income above $26,000 per annum (88.9%). Levels of physical activity were generally low, with only 28.4 % of participants reporting sufficient total activity and 16% of participants reporting sufficient planned (leisure-time) activity. The sample mean for depressive symptom scores measured by the Hospital Anxiety and Depression Scale (HADS-D) was 6.38 (¡¾ 2.55). The mean depressive symptom scores for participants who reported total moderate-to-vigorous activity levels of sufficient, insufficient, and none, were 5.43 (¡¾ 1.56), 5.82 (¡¾ 1.77) and 7.63 (¡¾ 3.25), respectively. Hierarchical multivariable linear regression modelling indicated that after controlling for covariates, a statistically significant difference of 1.09 points was observed between mean depressive symptom scores of participants who reported sufficient total physical activity, compared with participants who reported they were engaging in no moderate-to-vigorous activity in a typical week (p = 0.05) but this did not reach the criteria for a clinically meaningful difference. Total physical activity was contributed 2.2% to the total 30.3% of explained variance within this model. The other main contributors to explained variance in multivariable regression models were anxiety symptom scores and the number of existing children. Further, a trend was observed between higher levels of planned sitting behaviour and higher depressive symptom scores (p = 0.06); this correlation was not clinically meaningful. Planned sitting contributed 3.2% to the total 31.3 % of explained variance. The number of regression covariates and limited sample size led to a less than ideal ratio of covariates to participants, probably attenuating this relationship. Specific information about the sitting-based activities in which participants engaged may have provided greater insight about the relationship between planned sitting and depressive symptoms, but these data were not captured by the present study. Conclusions The finding that higher levels of physical activity were associated with lower levels of depressive symptoms is consistent with the current body of existing literature in pregnant women, and with a larger body of evidence based in general population samples. Although this result was not considered clinically meaningful, the criterion for a clinically meaningful result was an a priori decision based on quality of life literature in non-pregnant populations and may not truly reflect a difference in symptoms that is meaningful to pregnant women. Further investigation to establish clinically meaningful criteria for continuous depressive symptom data in pregnant women is required. This result may have implications relating to prevention and management options for depression during pregnancy. The observed trend between planned sitting and depressive symptom scores is consistent with literature based on leisure-time sitting behaviour in general population samples, and suggests that further research in this area, with larger samples of pregnant women and more specific sitting data is required to explore potential associations between activities such as television viewing and depressive symptoms, as this may be an area of behaviour that is amenable to modification

DIRECTED DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO PANCREATIC ENDOCRINE PROGENITORS

Diabetes is becoming increasingly prevalent in North America, highlighting the necessity to create a renewable source of pancreatic P-cells for therapeutic purposes. The current study was undertaken to explore the hypothesis that ectopic expression of lineage­ determining transcription factors could generate a source of proliferative, homogenous pancreatic progenitor cells capable of subsequent differentiation to functional endocrine cell types, including P-cells. We validated transgenic systems for transgene delivery in HESC and subsequently generated and analyzed gene expression patterns in transgenic cell populations engineered to constitutively express pancreatic transcription factors PAX4, PDX1, or NGN3. We demonstrate that ectopic expression of either PAX4 or PDX1 is not sufficient to specify pancreatic cells from human embryonic stem cells or definitive endoderm cells. In contrast, ectopic NGN3 expression specifies pancreatic endocrine cells from definitive endoderm. In conclusion, this study provides proof of principle of the ability to generate pancreatic endocrine cells using transcription factor overexpression in human embryonic stem cell

Re-Considering State-Society Dynamics in Turkey’s Kurdish Southeast

State-society relations in Turkey’s Kurdish southeast have long been characterized as pitting a powerful and determined state against a reluctant, resistant, but largely overwhelmed population. Such an image of a binary, state versus society relationship is readily substantiated by a myriad historical and contemporary examples: multiple Kurdish rebellions against the central state, cultural and political dissent articulated from poetry to political parties, emergency rule law in Kurdish-major..

Evaluation of Food Access and Food Security Concerns Among Public Health Housing Residents

Background: In 2012, food insecurity affected 14.5% of the households in the U.S and 20% in the state of Georgia. Individuals who are food-insecure can experience malnutrition, and social and physical problems. The purpose of this study was to assess food access and security concerns in two counties of the North Central Health District 5-2 (NCHD 5-2) in Georgia and to aid in devising interventions to increase food access and reduce food insecurity. Methods: Data collection involved surveying 399 public housing residents within two NCHD 5-2 counties using the Household Food Security Survey developed by the US Department of Agriculture. The survey contained 24 questions focusing on demographics and household food status and on the severity and prevalence of food access and security. Results: Of the 399 participants, 91.9% reported annual household incomes less than $30,000; 61% (n = 244) reported receiving Supplemental Nutrition Assistance Program (SNAP/food stamps) benefits, 11% (n = 46) received Women, Infants, and Children (WIC) benefits, and 3.3% (n = 13) received Temporary Assistance for Needy Families (TANF). Results for food security status (whether or not families have access to food at all times) showed that 7.3% (n = 29) were classified as high food secure, 22.8% (n = 91) as marginally food secure, 30.6% (n = 122) as low food secure, and 39.3% (n = 157) as very low food secure. Conclusions: Most of the residents with some form of food insecurity received government food assistance, yet still identified as being unable to feed themselves or their families for the month. Recommendations to evaluate this problem include additional research and implementation of public health efforts to address food access and insecurity through policy changes and implementation of programs

Development of octopus aquaculture: Rearing, handling and systems design for Octopus tetricus commercial aquaculture FRDC Project No 2009/206

The following document ‘Development of octopus aquaculture, rearing, handling and systems designs for Octopus tetricus commercial aquaculture’ contains protocols developed during the FRDC project 2009/206. These protocols encompass the most up-to-date rearing, handling and systems designs for Octopus tetricus commercial aquaculture. These protocols are the result of extensive research and development work carried out over the past four years by the Department of Fisheries, Western Australia and summarised in the final project report

Development of octopus aquaculture Final Report FRDC Project No 2009/206

During the past four years, the marine aquaculture group at the WA Fisheries and Marine Research Laboratories (WAFMRL) in collaboration with Occoculture Pty Ltd (subsidiary of Fremantle Octopus) and as a part of the FRDC project ‘Octopus Aquaculture Development’, investigated the potential of octopus aquaculture in Western Australia. Hatchery methods were developed in an attempt to close the life cycle. Advanced systems, rearing and feeding protocols were established for ranching Octopus tetricus juveniles

Declarative referential gesturing in a wild chimpanzee (Pan troglodytes)

Humans are argued to be unique in their ability and motivation to share attention with others about external entities—sharing attention for sharing’s sake. Indeed, in humans, using referential gestures declaratively to direct the attention of others toward external objects and events emerges in the first year of life. In contrast, wild great apes seldom use referential gestures, and when they do, it seems to be exclusively for imperative purposes. This apparent species difference has fueled the argument that the motivation and ability to share attention with others is a human-specific trait with important downstream consequences for the evolution of our complex cognition [M. Tomasello, Becoming Human (2019)]. Here, we report evidence of a wild ape showing a conspecific an item of interest. We provide video evidence of an adult female chimpanzee, Fiona, showing a leaf to her mother, Sutherland, in the context of leaf grooming in Kibale Forest, Uganda. We use a dataset of 84 similar leaf-grooming events to explore alternative explanations for the behavior, including food sharing and initiating dyadic grooming or playing. Our observations suggest that in highly specific social conditions, wild chimpanzees, like humans, may use referential showing gestures to direct others’ attention to objects simply for the sake of sharing. The difference between humans and our closest living relatives in this regard may be quantitative rather than qualitative, with ramifications for our understanding of the evolution of human social cognition

Linking Monitoring and Data Analysis to Predictions and Decisions for the Range-wide Eastern Black Rail Status Assessment

The US Fish and Wildlife Service (USFWS) has initiated a re-envisioned approach for providing decision makers with the best available science and synthesis of that information, called the Species Status Assessment (SSA), for endangered species decision making. The SSA report is a descriptive document that provides decision makers with an assessment of the current and pre - dicted future status of a species. These analyses support all manner of decisions under the US Endangered Species Act, such as listing, reclassification, and recovery planning. Novel scientific analysis and predictive modeling in SSAs could be an important part of rooting conservation decisions in current data and cutting edge analytical and modeling techniques. Here, we describe a novel analysis of available data to assess the current condition of eastern black rail Laterallus jamai censis jamai cen sis across its range in a dynamic occupancy analysis. We used the results of the ana lysis to develop a site occupancy projection model where the model parameters (initial occupancy, site persistence, colo nization) were linked to environmental covariates, such as land management and land cover change (sea-level rise, development, etc.). We used the projection model to predict future status under multiple sea-level rise and habitat management scenarios. Occupancy probability and site colonization were low in all analysis units, and site persistence was also low, suggesting low resiliency and redundancy currently. Extinction probability was high for all analysis units in all simulated scenarios except one with significant effort to preserve existing habitat, suggesting low future re siliency and redundancy. With the results of these data analyses and predictive models, the USFWS concluded that protections of the Endangered Species Act were warranted for this subspecies

Impact of gastric per‐oral endoscopic myotomy on static and dynamic pyloric function in gastroparesis patients

BackgroundFunctional Lumen Imaging Probe (EndoFLIP) tests typically measure static pyloric parameters, but the pylorus exhibits phasic variations on manometry. Dynamic changes in pyloric function have not been quantified using EndoFLIP, and the impact of Gastric Per‐Oral Endoscopic Myotomy (G‐POEM) on static and dynamic pyloric activity in gastroparesis is unknown.MethodsEndoFLIP balloon inflation to 30, 40, and 50 mL was performed to measure mean, maximum, and minimum values and variability in pyloric diameter and distensibility before and after G‐POEM in 20 patients with refractory gastroparesis. The impact of phasic contractions on these pyloric measures was compared.Key ResultsG‐POEM increased mean (P < .0001) and maximum (P = .0002) pyloric diameters and mean (P = .02) and maximum (P = .02) pyloric distensibility on 50 mL EndoFLIP inflation but not intraballoon pressures or minimum diameters or distensibility. Temporal variability of pyloric diameter (P = .02) and distensibility (P = .02) also increased after G‐POEM. Phasic coupled contractions propagating from the antrum through the pylorus were observed in 37.5% of recordings; other phasic activity including isolated pyloric contractions were seen in 23.3%. Variability of pyloric diameter and distensibility tended to be higher during recordings with phasic activity. Some pyloric responses to G‐POEM were influenced by age, gastroparesis etiology, gastric emptying, and prior botulinum toxin injection.Conclusions & InferencesPyloric activity exhibits dynamic changes on EndoFLIP testing in gastroparesis. G‐POEM increases maximal but not minimal diameter and distensibility with increased variations, suggesting this therapy enhances pyloric opening but may not impair pyloric closure. Phasic pyloric contractions contribute to variations in pyloric activity.We employed Functional Lumen Imaging Probe (EndoFLIP)tests toshowincreases in pyloric diameter and variability of diameter after gastricperoralendoscopicmyotomy(G‐POEM ingastroparesis patients (left graphs). Variability of pyloric activity was noted before and after G‐POEM which was partly due to propagated antropyloriccontractions (3‐D plot on right) detected by EndoFLIP.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/163489/2/nmo13892_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/163489/1/nmo13892.pd