Multi-collector Isotope Ratio Mass Spectrometer -- Operational Performance Report

This report describes the operational testing of a new magnetic sector mass spectrometer that utilizes seven full-sized discrete dynode electron multipliers operating simultaneously. The instrument includes a newly developed ion dispersion lens that enables the mass dispersed individual isotope beams to be separated sufficiently to allow a full-sized discrete dynode pulse counting multiplier to be used to measure each isotope beam. The performance of the instrument was measured using SRM 996 (244Pu spike) at loadings of 2.4 and 12 fg on resin beads and with SRM 4350B Columbia River Sediment samples. The measured limit of detection (3s) for 240Pu was 3.4 attograms for SRM 996. The limit of quantitation (LOQ), defined as 10 s, was 11.2 attograms. The measured concentration of 239Pu in the CRS standard was 152 ± 6 fg/g

Data for First Responder Use of Photoionization Detectors for Vapor Chemical Constituents

First responders need appropriate measurement technologies for evaluating incident scenes. This report provides information about photoionization detectors (PIDs), obtained from manufacturers and independent laboratory tests, and the use of PIDs by first responders, obtained from incident commanders in the United States and Canada. PIDs are valued for their relatively low cost, light weight, rapid detection response, and ease of use. However, it is clear that further efforts are needed to provide suitable instruments and decision tools to incident commanders and first responders for assessing potential hazardous chemical releases. Information provided in this report indicates that PIDs should always be part of a decision-making context in which other qualitative and more definitive tests and instruments are used to confirm a finding. Possible amelioratory actions ranging from quick and relatively easy fixes to those requiring significant additional effort are outlined in the report

Excavations at Gournia, 2010-2012

This article presents previous research at Gournia, the overall goals of our project, a new plan of the settlement, and our 2010-2012 excavations in eight areas: the Pit House, the Northwest Area, the North Cemetery, North Trench, the Northeast Area, House Aa, several rooms in the palace, and House He. Analytical sections discuss the textual evidence; the painted plasters; and the botanical remains. Our excavations indicate that Gournia was first settled in the Final Neolithic period and grew into an industrial town by the Protopalatial period. Following a Middle Minoan II destruction, the town was reorganized in Middle Minoan IIIA to include the palace, which in Late Minoan IB employed Linear A

Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking

The potential of the diverse chemistries present in natural products (NP) for biotechnology and medicine remains untapped because NP databases are not searchable with raw data and the NP community has no way to share data other than in published papers. Although mass spectrometry techniques are well-suited to high-throughput characterization of natural products, there is a pressing need for an infrastructure to enable sharing and curation of data. We present Global Natural Products Social molecular networking (GNPS, http://gnps.ucsd.edu), an open-access knowledge base for community wide organization and sharing of raw, processed or identified tandem mass (MS/MS) spectrometry data. In GNPS crowdsourced curation of freely available community-wide reference MS libraries will underpin improved annotations. Data-driven social-networking should facilitate identification of spectra and foster collaborations. We also introduce the concept of ‘living data’ through continuous reanalysis of deposited data

Multi-collector Isotope Ratio Mass Spectrometer -- Operational Performance Report

This report describes the operational testing of a new magnetic sector mass spectrometer that utilizes seven full-sized discrete dynode electron multipliers operating simultaneously. The instrument includes a newly developed ion dispersion lens that enables the mass dispersed individual isotope beams to be separated sufficiently to allow a full-sized discrete dynode pulse counting multiplier to be used to measure each isotope beam. The performance of the instrument was measured using SRM 996 (244Pu spike) at loadings of 2.4 and 12 fg on resin beads and with SRM 4350B Columbia River Sediment samples. The measured limit of detection (3s) for 240Pu was 3.4 attograms for SRM 996. The limit of quantitation (LOQ), defined as 10 s, was 11.2 attograms. The measured concentration of 239Pu in the CRS standard was 152 ± 6 fg/g

Solid Dielectric Transmission Lines for Pulsed Power

This paper documents recent work developing solid dielectric transmission lines for sub-microsecond, 100 kV class compact pulsed power systems. Polymer-ceramic nanocomposite materials have demonstrated sub-microsecond discharge capability in parallel plate capacitors and transmission lines [1, 2]. With a dielectric constant of approximately 50, the propagation velocity is 2.5 cm/ns, necessitating lines of several meters length to achieve \u3e 100 ns pulse lengths. By folding the line in a fashion analogous to ceramic multilayer capacitors, the physical length of the line can be significantly shorter than the electrical length. We present the results of an experimental effort to develop a folded transmission line using a polymer-ceramic nanocomposite dielectric. The pulse length was somewhat shorter than expected based on a simple calculation using the geometry and the dielectric constant. Fully 3-D electromagnetic calculations were used to examine the role of the edges in curtailing the pulse length. Dielectric breakdown in this device occurred below the electric field threshold demonstrated in the prior work [1]. Improvements in the large scale fabrication of TiO2 beginning with nanoscale grains have opened the possibility for producing single layer high voltage devices. Given a dielectric constant approaching 140, transmission lines using nano-TiO2 can be considerably shorter than with other materials. Relatively thick, flat sheets of TiO2 have been fabricated for testing up to 50 kV. Several transmission lines, employing a serpentine electrode geometry, have been manufactured and tested. Testing up to several 10\u27s of kV has confirmed the operation of the lines according to the design. As expected, the triple point between the TiO2, electrode, and insulating medium has proven difficult to manage for high voltage operation. Several techniques to mitigate the effects of the triple point, including resistive grading at the edges of the electrodes, are discussed. Fully 3-D electromagnetic modeling is used to examine the effects of electrode geometry and composition on the performance of the lines

Early adaptive radiations of Aplodontoidea (Rodentia, Mammalia) on the Holarctic region: systematics, and phylogenetic and paleobiogeographic implications

International audienceThe Aplodontoidea, now restricted to only oneNorth American species (Aplodontia rufa), have shown awide Holarctic extension since the Upper Eocene. As theirfossil record is poor, their phylogenetic relationships andthe origins of their successive radiations remain unclear.We perform here phylogenetic analyses, primarily based ondental evidence (94 dental of 97 characters), restricted toPaleogene and early Miocene taxa (46 taxa) in order toavoid biases introduced by substantially derived (divergent)taxa. We confirm the inclusion of some problematicgenera such as Cedromus or Douglassciurus withinSciuroidea rather than in Aplodontoidea. Ephemeromysand Lophallomys appear as the most basal members of theAplodontoidea, and Epeiromys is the closest outgroup ofthe Sciuroidea-Aplodontoidea clade. The relationshipsamong the ‘‘prosciurines’’ remain unclear, with paraphyleticgenera such as Prosciurus and Haplomys. Theirdiagnoses are reevaluated and a new genus is described.The Aplodontidae, including the clade of the latter, andHaplomys liolophus display a dichotomy betweenAnsomyinae and Aplodontinae, the two crown groups. Thefirst clade formed by the European species argoviensis anddescendens (referred to a new genus) can be proposed as asister group of the species of Ansomys. The second branchof the dichotomy includes the European Plesispermophilusand Sciurodon as basal groups. The species of Parallomysdo not form a clade, and the genus appears paraphyletic.The last dichotomy separates the Allomys clade from the‘meniscomyine’ clade. Comparisons of the selected speciesallow consideration of their patterns of dental evolution(e.g. enlargement of P4, development of a metaloph—protoloph disto-mesial connection, of crescentic shape inmain cusps and ectoloph, of a buccal protruding compressedmesostyle, of a metastylid crest or an anterior spurof the hypoconid, etc.). The split between sciuroids andaplodontoids occurred in North America, and then aplodontoidsdispersed rapidly throughout the whole Holarcticregion. The first aplodontid adaptive radiation took placeeither in North America or in Asia. Periodic exchangesoccurred between Europe, Asia and North America, andthe last radiations (meniscomyines) were restricted inNorth America

Molecular Networking as a Dereplication Strategy

A major goal in natural product discovery programs is to rapidly dereplicate known entities from complex biological extracts. We demonstrate here that molecular networking, an approach that organizes MS/MS data based on chemical similarity, is a powerful complement to traditional dereplication strategies. Successful dereplication with molecular networks requires MS/MS spectra of the natural product mixture along with MS/MS spectra of known standards, synthetic compounds, or well-characterized organisms, preferably organized into robust databases. This approach can accommodate different ionization platforms, enabling cross correlations of MS/MS data from ambient ionization, direct infusion, and LC-based methods. Molecular networking not only dereplicates known molecules from complex mixtures, it also captures related analogs, a challenge for many other dereplication strategies. To illustrate its utility as a dereplication tool, we apply mass spectrometry-based molecular networking to a diverse array of marine and terrestrial microbial samples, illustrating the dereplication of 58 molecules including analogs