ユーモア ヒョウシュツ ガ ソーシャル サポート オヨビ ヒョウシュツシャ ノ セイシンテキ ケンコウ ニ アタエル エイキョウ : ユーモア ノ ケイタイ ニ チャクモク シテ

The purpose of this research was to examine the effect of humor on mental health and social support, focusing on the type of humor. The result of this study indicated that there was a significant difference between men and women only in offensive humor expression. Moreover, the high social support group indicated higher humor expression of all the forms than the low social support group. This result suggests that humor expression of all forms are useful to obtain social support. The low social support group had higher uneasiness and lower well-being than the high social support group. Therefore, social support is useful to promote the both of positive and negative mental health. It was indicated that three kinds of humor expressions affect social support in path analysis. Furthermore, social support affects happiness and uneasiness. In this research, it was shown that the influence process of humor on mental health was different according to the form of humor expression

Development of hydroxyapatite-coated nonwovens for efficient isolation of somatic stem cells from adipose tissues

Adipose-derived stem cells (ASCs) are an attractive cell source for cell therapy. Despite the increasing number of clinical applications, the methodology for ASC isolation is not optimized for every individual. In this study, we developed an effective material to stabilize explant cultures from small-fragment adipose tissues. Methods: Polypropylene/polyethylene nonwoven sheets were coated with hydroxyapatite (HA) particles. Adipose fragments were then placed on these sheets, and their ability to trap tissue was monitored during explant culture. The yield and properties of the cells were compared to those of cells isolated by conventional collagenase digestion. Results: Hydroxyapatite-coated nonwovens immediately trapped adipose fragments when placed on the sheets. The adhesion was stable even in culture media, leading to cell migration and proliferation from the tissue along with the nonwoven fibers. A higher fiber density further enhanced cell growth. Although cells on nonwoven explants could not be fully collected with cell dissociation enzymes, the cell yield was significantly higher than that of conventional monolayer culture without impacting stem cell properties. Conclusions: Hydroxyapatite-coated nonwovens are useful for the effective primary explant culture of connective tissues without enzymatic cell dissociation

Pluripotent Cell Models of Fanconi Anemia Identify the Early Pathological Defect in Human Hemoangiogenic Progenitors

Fanconi anemia (FA) is a disorder of genomic instability characterized by progressive bone marrow failure (BMF), developmental abnormalities, and an increased susceptibility to cancer. Although various consequences in hematopoietic stem/progenitor cells have been attributed to FA-BMF, the quest to identify the initial pathological event is still ongoing. To address this issue, we established induced pluripotent stem cells (iPSCs) from fibroblasts of six patients with FA and FANCA mutations. An improved reprogramming method yielded iPSC-like colonies from all patients, and iPSC clones were propagated from two patients. Quantitative evaluation of the differentiation ability demonstrated that the differentiation propensity toward the hematopoietic and endothelial lineages is already defective in early hemoangiogenic progenitors. The expression levels of critical transcription factors were significantly downregulated in these progenitors. These data indicate that the hematopoietic consequences in FA patients originate from the early hematopoietic stage and highlight the potential usefulness of iPSC technology for elucidating the pathogenesis of FA-BMF

Installation Effect of Distributed Power and Heat Source System for Housing

This paper presents effects of housing electricity and heat source equipments. We studied about energy saving effects of 4 equipments, CO2 Heat Pump water heater(CO2-HP), Micro Gas Engine Co-Generation System(GE-μCGS), Polymer Electrolyte Fuel Cell Co-Generation System(PEFC-CGS), and Solid Oxide Fuel Cell Co-Generation System(SOFC-CGS). As the result, we have found that energy consumption and CO2 emission reduction rate is the highest in the case of SOFC-CGS. Energy consumption is reduced by 17-20% using SOFC-CGS on Electric Power Load Tracking Operation, and 44-47% on Rated Operation. And CO2 emission is reduced by 6-21% on Electric Power Load Tracking Operation and 23-47% on Rated Operation

Upstream open reading frame-mediated upregulation of ANAC082 expression in response to nucleolar stress in Arabidopsis

Perturbations in ribosome biogenesis cause a type of cellular stress called nucleolar or ribosomal stress, which triggers adaptive responses in both animal and plant cells. The Arabidopsis ANAC082 transcription factor has been identified as a key mediator of the plant nucleolar stress response. The 5 '-untranslated region (5 '-UTR) of ANAC082 mRNA contains an upstream ORF (uORF) encoding an evolutionarily conserved amino acid sequence. Here, we report that this uORF mediates the upregulation of ANAC082 expression in response to nucleolar stress. When transgenic Arabidopsis plants containing a luciferase reporter gene under the control of the ANAC082 promoter and 5 '-UTR were treated with reagents that induced nucleolar stress, expression of the reporter gene was enhanced in a uORF sequence-dependent manner. Additionally, we examined the effect of an endoplasmic reticulum (ER) stress-inducing reagent on reporter gene expression because the closest homolog of ANAC082 in Arabidopsis, ANAC103, is involved in the ER stress response. However, the ANAC082 uORF did not respond to ER stress. Interestingly, although ANAC103 has a uORF with an amino acid sequence similar to that of the ANAC082 uORF, the C-terminal sequence critical for regulation is not well conserved among ANAC103 homologs in Brassicaceae. Transient expression assays revealed that unlike the ANAC082 uORF, the ANAC103 uORF does not exert a sequence-dependent repressive effect. Altogether, our findings suggest that the ANAC082 uORF is important for the nucleolar stress response but not for the ER stress response, and that for this reason, the uORF sequence-dependent regulation was lost in ANAC103 during evolution