Adsorption and Desorption of Bioactive Proteins on Hydroxyapatite for Protein Delivery Systems

Hydroxyapatite (HA) is a precursor of bone and has been studied as a biomaterial. We attempted HA to apply to protein delivery systems. In this study, the association and dissociation properties of two types of bioactive proteins, cytochrom c and insulin, to HA were investigated. Cytochrom c was less associated with HA than insulin, which was easily released from it. However, the release of insulin from HA was slow. Insulin was released from HA at pH 7.4 more rapidly than at pH 3. The association and dissociation properties might be influenced by the size, solubility and net charge of protein. HA is a potential protein carrier with controlled release

Baroque Elements In The Piano Sonata, Opus 9 By Paul Creston

Paul Creston (1906-1985) was one of the most significant American composers from the middle of the twentieth century. Though Creston maintained elements of the nineteenth-century Romantic tradition and was categorized as a “Neo-Romantic” or “20th-century traditionalist,” many of Creston’s compositions contain elements of Baroque music. His Piano Sonata, Opus 9 provides significant examples of Baroque elements, while already foreshadowing his mature style. The purpose of this study is to explore Baroque elements in the compositional language of Paul Creston’s Piano Sonata, Opus 9. All four movements of the Piano Sonata will be examined in regards to its stylistic features associated with Baroque practices. These features mainly consist of rhythm, texture, imitative writing, and repeated phrase structure. Each category of the study will include comparisons of Domenico Scarlatti’s keyboard sonatas with Creston’s sonata. Through an examination of the Piano Sonata and its Baroque elements, this study hopes to inspire renewed interest in the work among musicians and to help the performer give a more stylistically coherent, and accurate, performance

Probable association of T Tauri stars with the L1014 dense core

Using the Wide Field Grism Spectrograph 2 (WFGS2), we have carried out slit-less spectroscopy, g'r'i' photometry, and slit spectroscopy on the L1014 dense core. We detected three Halpha emission line stars. We interpret one as weak-line T Tauri star (WTTS) and the others as classical T Tauri stars (CTTS). Since their g'-i' colors and/or classified spectral types are consistent with those of T Tauri stars and two of them show less extinction than the cloud, these three stars are likely to be T Tauri stars associated with L1014. Adopting an age range for T Tauri stars, 1-10 Myr, the color-magnitude diagram suggests a distance of ~400-900 pc, rather than the previously assumed distance, 200 pc. This could strongly affect on the mass estimate of L1014-IRS, which is thought to be either a very young protostar or proto-brown dwarf.Comment: 5 pages, 5 figures, to be published in Vol.58, No.5, October 25, 200

Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR

<p>Abstract</p> <p>Background</p> <p>Lung cancers are the most common type of human malignancy and are intractable. Lung cancers are generally classified into four histopathological subtypes: adenocarcinoma (AD), squamous cell carcinoma (SQ), large cell carcinoma (LC), and small cell carcinoma (SC). Molecular biological characterization of these subtypes has been performed mainly using DNA microarrays. In this study, we compared the gene expression profiles of these four subtypes using twelve human lung cancer cell lines and the more reliable quantitative real-time PCR (qPCR).</p> <p>Results</p> <p>We selected 100 genes from public DNA microarray data and examined them by DNA microarray analysis in eight test cell lines (A549, ABC-1, EBC-1, LK-2, LU65, LU99, STC 1, RERF-LC-MA) and a normal control lung cell line (MRC-9). From this, we extracted 19 candidate genes. We quantified the expression of the 19 genes and a housekeeping gene, <it>GAPDH</it>, with qPCR, using the same eight cell lines plus four additional validation lung cancer cell lines (RERF-LC-MS, LC-1/sq, 86-2, and MS-1-L). Finally, we characterized the four subtypes of lung cancer cell lines using principal component analysis (PCA) of gene expression profiling for 12 of the 19 genes (<it>AMY2A</it>, <it>CDH1</it>, <it>FOXG1</it>, <it>IGSF3</it>, <it>ISL1</it>, <it>MALL</it>, <it>PLAU</it>, <it>RAB25</it>, <it>S100P</it>, <it>SLCO4A1</it>, <it>STMN1</it>, and <it>TGM2</it>). The combined PCA and gene pathway analyses suggested that these genes were related to cell adhesion, growth, and invasion. <it>S100P </it>in AD cells and <it>CDH1 </it>in AD and SQ cells were identified as candidate markers of these lung cancer subtypes based on their upregulation and the results of PCA analysis. Immunohistochemistry for S100P and RAB25 was closely correlated to gene expression.</p> <p>Conclusions</p> <p>These results show that the four subtypes, represented by 12 lung cancer cell lines, were well characterized using qPCR and PCA for the 12 genes examined. Certain genes, in particular <it>S100P </it>and <it>CDH1</it>, may be especially important for distinguishing the different subtypes. Our results confirm that qPCR and PCA analysis provide a useful tool for characterizing cancer cell subtypes, and we discuss the possible clinical applications of this approach.</p