Amperometric biosensor for cholesterol based on novel nanocomposite array gold nanoparticles/acetone‐extracted propolis/multiwall carbon nanotubes/gold

In this reported work, the electrochemical behaviour of gold electrodes modified with gold nanoparticles, acetone-extracted propolis, multiwall carbon nanotubes and cholesterol oxidase was established for the detection of hydrogen peroxide by using cyclic voltammetry and amperometric techniques. The obtained results confirmed that the current enzymatic biosensor exhibits a fast, highly sensitive, and cost-effective detection of cholesterol. Cholesterol in the concentration range of 0.15-0.55 mmol l(-1) was determined with a detection limit of 4.9 x 10(-5) mol l(-1) by the amperometric method, and the sensitivity of the proposed method was found to be 17.38 mu A/mmol l(-1). Normal electroactive species such as ascorbic acid and glucose in the presence of the constant concentration of cholesterol in the samples do not interfere with the determination

An audit of diabetes control, dietary management and quality of life in adults with type 1 diabetes mellitus, and a comparison with nondiabetic subjects

Objectives:  The study\u27s objective was to audit current diet and disease management in a community-based sample of people with type 1 diabetes. Methods:  The study involved adults with type 1 diabetes and control subjects. Reported amounts of dietary intake were collected. Indices of diabetes control were determined by standard methods. Quality of life of both groups was assessed with appropriate measures. Results were compared between two groups. Results:  Mean HbA1c concentration was 8.5% (SD 2.21%) for women with diabetes and 8.6% (SD 1.91%) for men. There was no significant difference between the diabetic and control subjects in self-reported energy intake and macronutrient intake, with the exception that the contribution of saturated fatty acid to energy intake was higher in male controls than in male diabetics. There was no association between dietary intakes and glycemic control in diabetic subjects. Anthropometric measurements, blood pressure and plasma lipids in patients were within normal range and not significantly different from the controls. Plasma fibrinogen concentration was higher in patients. Diabetic subjects did not have a diminished quality of life. Conclusions:  Dietary management in these patients was generally focused on controlling carbohydrate intake. Most had suboptimal diabetes control