He Repercussions of Childhood Trauma on Posttraumatic Stress: the Mediating Effects of Dissociation and Emotion Dysregulation

The present study explored the mediating effects of dissociation and emotion dysregulation on the relationship between different types of childhood trauma and symptoms of posttraumatic stress. Participants were 181 undergraduate students at Cleveland State University, who competed measures of childhood trauma (emotional abuse, physical abuse, sexual abuse, and general trauma), posttraumatic stress symptoms, dissociation, and emotion dysregulation. Multiple mediation analyses were conducted to examine the model proposed in this study. The results of this study revealed that all trauma types significantly predicted adulthood posttraumatic stress. The relationship between emotional abuse and posttraumatic stress was mediated through both dissociation and emotion dysregulation. Specific indirect effects emerged through dissociation on physical abuse and emotional abuse, and through emotion dysregulation on general trauma, and posttraumatic stress. These findings support prior research that associates posttraumatic stress symptoms to traumatic childhood experiences. Furthermore, the results indicate differential psychopathological outcomes related to type of traumatic experience in early life

Listening to Voices: Understanding Chinese Students' Journey at a Canadian University

This research explored the motivations of Chinese students to study in Canada and analyzed their cultural adaptation process at a Canadian university. This served the Canadian educational administrators and prospective Chinese students who were interested in studying in Canada. The following three research questions provided the direction for this study: 1 What are Chinese students’ motivations to study in Canada? 2 What are Chinese students’ experiences at Canadian universities and how do their motivations shape their journey in Canada? 3 What challenges and opportunities do Chinese students face in their cultural adaptation, and what strategies do Chinese students think can be applied by Canadian educational administrators to assist Chinese students? A qualitative approach was the research method utilized in this study. Using semi-structured life-story interviews, data were collected from a purposeful sample of four Chinese graduate students studying at a Canadian university. Utilizing a narrative research method, from an emic perspective, I listened to Chinese students’ voices and their life stories and to explore deeply their experiences in Canada. Three theoretical frameworks were employed to analyze the data. The synthesis model was used to explore the decision making process of participants to come to Canada; the push-pull model was employed to identify the factors attracting people to move to another country; and Self- Determined Theory was used to analyze the connection between participants’ motivations to study abroad and their subsequent cultural adaptation. Data collected from semi-structured interviews with four participants indicated that Chinese students were easily attracted to study in Canada by its favorable and tolerant environment, positive reputation for diversity, high quality post-secondary institutions and the possibility of securing a visa relatively easily. In their decision making process, Chinese students were influenced by some important factors: student characteristics, parental factors, and other external important factors. Among those factors influencing Chinese students’ decisions to move to Canada, family was recognized a very important factor and participants’ narratives suggested that having family connections in Canada could be a helpful factor for Chinese students to adapt to a new culture, which was reported as a best case scenario in this research. However, family also acted as a barrier for Chinese students to acquire English proficiency and interact with local people. Findings from this study supported earlier research that suggested students who were more self-determined to study abroad were more likely to succeed academically and had fewer traumas in navigating cross-cultural adaptations

Are Cell Death Proteins/Antigens Found on Interdigital Cells Dying During Limb Development Expressed in a Simple Organism Such As Tetrahymena?

Numerous studies have been published that describe the genes and proteins that control cell death in various biological systems including normal embryonic development and in disease such as cancer. We describe attempts to look at a possible conserved cell death antigen in the simple organism Tetrahymena, using a unique monoclonal antibody that recognizes only dying cells in the chick limb. The main impetus for the research is to answer the question; does the cell death process have key proteins that exist in the dying process that can be modulated prior to the completion of the cell death process? Using various stimuli to induce cell death in tetrahymena thermophila including staurosporine, hypoxia and other know cell death modulators, we describe the preliminary methods used to verify that cells across two species may express conserved cell death proteins at certain times during the death process. The goal is to demonstrate that normal interdigit cell death is an ideal system for isolating programmed cell death antigens and provides a way to identify common mediators/markers in other model systems such as tetrahymena thermophila

Engineering the genomes of wild insect populations: Challenges, and opportunities provided by synthetic Medea selfish genetic elements

Advances in insect transgenesis and our knowledge of insect physiology and genomics are making it possible to create transgenic populations of beneficial or pest insects that express novel traits. There are contexts in which we may want the transgenes responsible for these traits to spread so that all individuals within a wild population carry them, a process known as population replacement. Transgenes of interest are unlikely to confer an overall fitness benefit on those who carry them. Therefore, an essential component of any population replacement strategy is the presence of a drive mechanism that will ensure the spread of linked transgenes. We discuss contexts in which population replacement might be desirable and the requirements a drive system must satisfy to be both effective and safe. We then describe the creation of synthetic Medea elements, the first selfish genetic elements synthesized de novo, with the capability of driving population replacement, in this case in Drosophila. The strategy used to create Drosophila Medea is applicable to a number of other insect species and the Medea system satisfies key requirements for scientific and social acceptance. Finally, we highlight several challenges to implementing population replacement in the wild

The Different Artificial Sweeteners and Their Effects on Endothelial Cell/Blood Vessel Health: Possible Implications for Ringing in the Ear

Abstract: Background: Tinnitus, a condition whose remarkable symptom is ringing in the ear (RIE), is a problem plaguing people all around the world in varying degrees of severity, though it is most common and severe in older populations. Literature is lacking on its etiology. Therefore, it is difficult to diagnose and treat. Several possible components could play a role in the development of tinnitus including neurological, physiological, traumatic, dietary and vascular factors. No factor has yet been definitively linked to the development of tinnitus. Vascular health can be significantly impacted by diet- especially in regard to sugars. As artificial sweeteners are used widely in the American diet, they may play a significant role in vascular health. Objective: This project aims to investigate a possible connection between artificial sugars’ impact on vascular health and complaints of RIE among age groups through a patient survey and laboratory experiments. Methods: A survey assessing individuals’ demographic information, subjective severity of RIE and reported artificial sweetener consumption will be distributed to audiologists’ offices in major Ohio cities, via Qualtrics, where patients will complete them. Data collected will be analyzed for interrelationships among sugar intake, age and severity of RIE. In the laboratory, an ELISA assay will evaluate the effects of artificial sweeteners on endothelial cells- the same cells that comprise blood vessels- through quantifying stimulation of the Tie-2 survival and angiogenesis pathway via the cellular messenger pAKT

Mandibulofacial Dysostosis Attributed to a Recessive Mutation of CYP26C1 in Hereford Cattle

In spring 2020, six Hereford calves presented with congenital facial deformities attributed to a condition we termed mandibulofacial dysostosis (MD). Affected calves shared hallmark features of a variably shortened and/or asymmetric lower mandible and bilateral skin tags present 2–10 cm caudal to the commissure of the lips. Pedigree analysis revealed a single common ancestor shared by the sire and dam of each affected calf. Whole-genome sequencing (WGS) of 20 animals led to the discovery of a variant (Chr26 g. 14404993T\u3eC) in Exon 3 of CYP26C1 associated with MD. This missense mutation (p.L188P), is located in an α helix of the protein, which the identified amino acid substitution is predicted to break. The implication of this mutation was further validated through genotyping 2 additional affected calves, 760 other Herefords, and by evaluation of available WGS data from over 2500 other individuals. Only the a_ected individuals were homozygous for the variant and all heterozygotes had at least one pedigree tie to the suspect founder. CYP26C1 plays a vital role in tissue-specific regulation of retinoic acid (RA) during embryonic development. Dysregulation of RA can result in teratogenesis by altering the endothelin-1 signaling pathway affecting the expression of Dlx genes, critical to mandibulofacial development. We postulate that this recessive missense mutation in CYP26C1 impacts the catalytic activity of the encoded enzyme, leading to excess RA resulting in the observed MD phenotype

De Novo Assembly of the Complete Genome of an Enhanced Electricity-Producing Variant of Geobacter sulfurreducens Using Only Short Reads

State-of-the-art DNA sequencing technologies are transforming the life sciences due to their ability to generate nucleotide sequence information with a speed and quantity that is unapproachable with traditional Sanger sequencing. Genome sequencing is a principal application of this technology, where the ultimate goal is the full and complete sequence of the organism of interest. Due to the nature of the raw data produced by these technologies, a full genomic sequence attained without the aid of Sanger sequencing has yet to be demonstrated

Angiotensin-converting enzyme activity and inhibition in dogs with cardiac disease and an angiotensin-converting enzyme polymorphism

OBJECTIVE The objective of this study was to evaluate angiotensin-converting enzyme (ACE) activity in dogs and with and without an ACE polymorphism in the canine ACE gene, before and after treatment with an ACE inhibitor. METHODS Thirty-one dogs (20 wild-type, 11 ACE polymorphism) with heart disease were evaluated with ACE activity measurement and systolic blood pressure before and after administration of an ACE inhibitor (enalapril). RESULTS Median pre-treatment ACE activity was significantly lower for ACE polymorphism dogs than for dogs with the wild-type sequence ( P=0.007). After two weeks of an ACE inhibitor, ACE activity was significantly reduced for both genotypes (wild-type, P<0.0001; ACE polymorphism P=0.03); mean post-therapy ACE activity was no different between the groups. CONCLUSION An ACE polymorphism is associated with lower levels of ACE activity. Dogs with the polymorphism still experience suppression of ACE activity in response to an ACE inhibitor. It is possible that the genetic status and ACE activity of dogs may impact the response of dogs with this variant to an ACE inhibitor