27 research outputs found

    Arytmia z piek艂a rodem. Idiopatyczny p臋czkowy cz臋stoskurcz komorowy u m艂odej kobiety

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    Idiopathic left fascicular ventricular tachycardia (ILFVT) is characterized by right bundle branch block (RBBB) morphology and left axis deviation. We report a case of ILFVT in a young 27-year-old female patient presenting with a narrow complex tachycardia resistant to vagal maneuvers, adenosine, lidocaine, and electrical cardioversion.Idiopathic left fascicular ventricular tachycardia (ILFVT) is characterized by right bundle branch block (RBBB) morphology and left axis deviation. We report a case of ILFVT in a young 27-year-old female patient presenting with a narrow complex tachycardia resistant to vagal maneuvers, adenosine, lidocaine, and electrical cardioversion

    Application of photochemical parameters and several indices based on phenotypical traits to assess intraspecific variation of oat (Avena sativa L.) tolerance to drought

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    Functionality of the photosynthetic system under water stress is of major importance in drought tolerance. Oat (Avena sativa L.) doubled haploid (DH) lines obtained by pollination of F_{1} oat crosses with maize were used to assess the differences in plant genotypic response to soil drought. The investigations were based on the measurements of gas exchange and chlorophyll a fluorescence kinetics. Drought was applied to 17-day-old seedlings by withholding water for 14 days and subsequent plant recovery. Non-stressed optimally watered plants served as controls. Yield components were determined when plants reached full maturity. It was shown differences among the oat lines with respect to drought stress susceptibility (SI) and stress tolerance index mean productivity and drought susceptibility index. Sensitivity to drought of individual DH lines was significantly different, as demonstrated by the correlation between drought susceptibility index and yield components, such as dry weight (GW) or grain number (GN) of the harvested plants. GW and GN were lower in drought-sensitive genotypes exposed to drought stress compared to those resistant to drought. The principal component analysis allow to separate three groups of lines differing in their sensitivity to drought stress and indicated that tolerance to drought in oat has a common genetic background

    Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.)

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    Background. The oat脳maize addition (OMA) lines are used for mapping of the maize genome, the studies of centromere-specific histone (CENH3), gene expression, meiotic chromosome behavior and also for introducing maize C4 photosynthetic system to oat. The aim of our study was the identification and molecular-cytogenetic characterization of oat 脳 maize hybrids. Methods. Oat DH lines and oat 脳 maize hybrids were obtained using the wide crossing of Avena sativa L. with Zea mays L. The plants identified as having a Grande- 1 retrotransposon fragment, which produced seeds, were used for genomic in situ hybridization (GISH). Results. A total of 138 oat lines obtained by crossing of 2,314 oat plants from 80 genotypes with maize cv. Waza were tested for the presence of maize chromosomes. The presence of maize chromatin was indicated in 66 lines by amplification of the PCR product (500 bp) generated using primers specific for the maize retrotransposon Grande-1. Genomic in situ hybridization (GISH) detected whole maize chromosomes in eight lines (40%). All of the analyzed plants possessed full complement of oat chromosomes. The number of maize chromosomes differed between the OMA lines. Four OMA lines possessed two maize chromosomes similar in size, three OMA-one maize chromosome, and one OMA-four maize chromosomes. In most of the lines, the detected chromosomes were labeled uniformly. The presence of six 45S rDNA loci was detected in oat chromosomes, but none of the added maize chromosomes in any of the lines carried 45S rDNA locus. Twenty of the analyzed lines did not possess whole maize chromosomes, but the introgression of maize chromatin in the oat chromosomes. Five of 66 hybrids were shorter in height, grassy type without panicles. Twenty-seven OMA lines were fertile and produced seeds ranging in number from 1-102 (in total 613). Sixty-three fertile DH lines, out of 72 which did not have an addition of maize chromosomes or chromatin, produced seeds in the range of 1-343 (in total 3,758). Obtained DH and OMA lines were fertile and produced seeds. Discussion. In wide hybridization of oat with maize, the complete or incomplete chromosomes elimination of maize occur. Hybrids of oat and maize had a complete set of oat chromosomes without maize chromosomes, and a complete set of oat chromosomes with one to four retained maize chromosomes
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