New visible-light-driven Bi2MoO6/Cs3Sb2Br9 heterostructure for selective photocatalytic oxidation of toluene to benzaldehyde

Herein, new Bi2MoO6/Cs3Sb2Br9 heterostructure (BiMo/CSB) was investigated for the first time as a visible-light-driven photocatalyst for C(sp3)–H bond activation using molecular oxygen as a green oxidant and toluene as a model substrate. The optimized BiMo/CSB photocatalyst exhibited enhanced toluene oxidation activity (2,346 μmol g-1h−1), which was almost two- and five-fold that of pristine CSB (1,165 μmol g-1h−1) and BiMo (482 μmol g-1h−1), respectively. The improved photocatalytic performance was essentially attributed to the formation of staggered band energy lineup in the BiMo/CSB hybrid, which promoted S-scheme charge transfer across the BiMo/CSB heterointerface as supported by ultraviolet photoelectron spectroscopy (UPS), density functional theoretical (DFT), time-resolve photoluminescence (TRPL), and photoelectrochemical studies. Spin–trapping electron paramagnetic resonance (EPR) and radical scavenging studies revealed that photoinduced hole, molecular oxygen, and superoxide radical are key active species in this photocatalytic system. The developed BiMo/CSB catalyst provided good selectivity toward benzaldehyde product (94–98 %), presumably due to the inhibiting effect of benzyl alcohol on benzaldehyde oxidation. No significant change in structure and morphology was observed for the spent catalyst, however small negative shift of Sb 3d and Bi 4f binding energy was found suggesting partial reduction of Sb3+ and Bi3+. This work not only provides a new visible-light-driven photocatalyst for C(sp3)–H bond activation but also opens the doors for exploitation of the conversion and functionalization of this inert bond toward the production of high value-added organic chemicals

Development of a microarray lateral flow strip test using a luminescent organic compound for multiplex detection of five mycotoxins

While lateral flow immunoassay (LFIA) is a simple technique that offers a rapid, robust, user friendly, and point-of-care test, its capacity for multiplex detection is rather limited. This study therefore combined the multiplexity of microarray technique and the simple and rapid characteristics of LFIA to enable simultaneous and quantitative detection of five mycotoxins, namely aflatoxin B1 (AFB1), deoxynivalenol (DON), fumonisin B1 (FUMB1), T-2 toxin (T-2), and zearalenone (ZON). In addition, we have synthesized a novel extra-large Stokes shift and strong fluorescence organic compound to be used as a reporter molecule which can be detected under UV light without light filter requirement. Many parameters including microarray spotting buffer, blocking buffer, and concentrations of mycotoxin antibodies were optimized for the microarray LFIA (μLFIA) construction. With the optimal conditions, the μLFIA could accurately and quantitatively detect multiple mycotoxins at the same time. The limits of detection of AFB1, DON, FUMB1, T-2, and ZON were 1.3, 0.5, 0.4, 0.4, and 0.9 ppb, respectively. The recoveries of these five mycotoxins were 70.7%–119.5% and 80.4%–124.8% for intra-assay and inter-assay, respectively. Combining the advantages of the novel reporter molecule and the multiplex capability of μLFIA test, this system could simultaneously detect multiple mycotoxins in one sample with high specificity and high sensitivity. Moreover, this system presents a promising affordable point-of-care platform to detect other analytes as well.</p

Dual-Analyte Fluorescent Sensor Based on [5]Helicene Derivative with Super Large Stokes Shift for the Selective Determinations of Cu²⁺ or Zn²⁺ in Buffer Solutions and Its Application in a Living Cell

A new fluorescent sensor, <b>M201-DPA</b>, based on [5]­helicene derivative was utilized as dual-analyte sensor for determination of Cu²⁺ or Zn²⁺ in different media and different emission wavelengths. The sensor could provide selective and bifunctional determination of Cu²⁺ in HEPES buffer containing Triton-X100 and Zn²⁺ in Tris buffer/methanol without interference from each other and other ions. In HEPES buffer, <b>M201-DPA</b> demonstrated the selective ON–OFF fluorescence quenching at 524 nm toward Cu²⁺. On the other hand, in Tris buffer/methanol, <b>M201-DPA</b> showed the selective OFF–ON fluorescence enhancement upon the addition of Zn²⁺, which was specified by the hypsochromic shift at 448 nm. Additionally, <b>M201-DPA</b> showed extremely large Stokes shifts up to ∼150 nm. By controlling the concentration of Zn²⁺ and Cu²⁺ in a living cell, the imaging of a HepG2 cellular system was performed, in which the fluorescence of <b>M201-DPA</b> in the blue channel was decreased upon addition of Cu²⁺ and was enhanced in UV channel upon addition of Zn²⁺. The detection limits of <b>M201-DPA</b> for Cu²⁺ and Zn²⁺ in buffer solutions were 5.6 and 3.8 ppb, respectively. Importantly, the Cu²⁺ and Zn²⁺ detection limits of the developed sensors were significantly lower than permitted Cu²⁺ and Zn²⁺ concentrations in drinking water as established by the U.S. EPA and WHO