DRCM: a disentangled representation network based on coordinate and multimodal attention for medical image fusion

Recent studies on medical image fusion based on deep learning have made remarkable progress, but the common and exclusive features of different modalities, especially their subsequent feature enhancement, are ignored. Since medical images of different modalities have unique information, special learning of exclusive features should be designed to express the unique information of different modalities so as to obtain a medical fusion image with more information and details. Therefore, we propose an attention mechanism-based disentangled representation network for medical image fusion, which designs coordinate attention and multimodal attention to extract and strengthen common and exclusive features. First, the common and exclusive features of each modality were obtained by the cross mutual information and adversarial objective methods, respectively. Then, coordinate attention is focused on the enhancement of the common and exclusive features of different modalities, and the exclusive features are weighted by multimodal attention. Finally, these two kinds of features are fused. The effectiveness of the three innovation modules is verified by ablation experiments. Furthermore, eight comparison methods are selected for qualitative analysis, and four metrics are used for quantitative comparison. The values of the four metrics demonstrate the effect of the DRCM. Furthermore, the DRCM achieved better results on SCD, Nabf, and MS-SSIM metrics, which indicates that the DRCM achieved the goal of further improving the visual quality of the fused image with more information from source images and less noise. Through the comprehensive comparison and analysis of the experimental results, it was found that the DRCM outperforms the comparison method

PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in <i>Escherichia coli</i>

Efficiency and fidelity are the key obstacles for genome editing toolboxes. In the present study, a PCR-based tandem repeat assisted genome editing (TRAGE) method with high efficiency and fidelity was developed. The design of TRAGE is based on the mechanism of repair of spontaneous double-strand breakage (DSB) via replication fork reactivation. First, cat-sacB cassette flanked by tandem repeat sequence was integrated into target site in chromosome assisted by Red enzymes. Then, for the excision of the cat-sacB cassette, only subculturing is needed. The developed method was successfully applied for seamlessly deleting, substituting and inserting targeted genes using PCR products. The effects of different manipulations including sucrose addition time, subculture times in LB with sucrose and stages of inoculation on the efficiency were investigated. With our recommended procedure, seamless excision of cat-sacB cassette can be realized in 48 h efficiently. We believe that the developed method has great potential for seamless genome editing in E. coli

Effects of Fe Contents on the Microstructure and Precipitate of Ti–Al–V Alloys Prepared by Direct Energy Deposition

This study investigated the influence of Fe content on the microstructure and mechanical properties of Ti–6Al–4V(TC4) + 25Ti alloys prepared by low-energy-density direct energy deposition (DED) technology. With the incorporation of the Fe elements, the α-Ti phases exhibited significant changes in size and morphology, while the numerous β-Ti phases and some triclinic-Ti precipitates were retained. With the refinement of the α-Ti phase, retainment of the β-Ti phase and the presence of triclinic-Ti precipitates, the mechanical properties of DED samples can be significantly improved compared with DED TC4 alloys. The room-temperature mechanical property tests showed that the ultimate tensile strength (UTS) of 3Fe + TC4 + 25Ti achieved 1298.64 ± 5.26 MPa with an elongation of 4.82% ± 0.20%, and the maximum elongation of 1Fe + TC4 + 25Ti reached 10.82% ± 0.82% with a UTS of 1076.95 ± 11.69 MPa. The strengthening mechanism of DED Ti-Al-V-Fe alloys were further discussed, providing new insights into the microstructure control and the composition design of additive manufacturing of Ti alloys

Empirical Analysis of Population Urbanization and Residents&rsquo; Life Satisfaction&mdash;Based on 2017 CGSS

As the greatest potential of domestic demand, new urbanization shoulders the important mission of improving the living standards of residents. Based on the theory of exploitation, this paper systematically established the theoretical relationships among population urbanization rate, human capital, family capital, and life satisfaction. Through the 2017 China Comprehensive Social Survey of 1940 micro-individuals for empirical analysis, the results show that: (1) the urbanization rate of the core explanatory variable has a significant and robust positive effect on individual life satisfaction and on human capital and family capital; (2) the urbanization rate of the core explanatory variable has a significant positive effect on human capital and family capital; (3) human capital and family capital have significant positive effects on life satisfaction; (4) in the heterogeneity discussion, the male capital accumulation is higher than the female, but life satisfaction is the opposite. With the increase in age, the individuals accumulated the highest human capital and family capital in 26&ndash;34 years old and reached the peak in life satisfaction after retirement in 60&ndash;83 years old. As far as regional differences are concerned, individual human capital, family capital, and life satisfaction are decreasing from the east to the west. The results of the study will help to establish a healthy and perfect regional urbanization and to enhance the mental health of residents by promoting talent development and advocating family-friendly construction

Empirical Analysis of Population Urbanization and Residents’ Life Satisfaction—Based on 2017 CGSS

As the greatest potential of domestic demand, new urbanization shoulders the important mission of improving the living standards of residents. Based on the theory of exploitation, this paper systematically established the theoretical relationships among population urbanization rate, human capital, family capital, and life satisfaction. Through the 2017 China Comprehensive Social Survey of 1940 micro-individuals for empirical analysis, the results show that: (1) the urbanization rate of the core explanatory variable has a significant and robust positive effect on individual life satisfaction and on human capital and family capital; (2) the urbanization rate of the core explanatory variable has a significant positive effect on human capital and family capital; (3) human capital and family capital have significant positive effects on life satisfaction; (4) in the heterogeneity discussion, the male capital accumulation is higher than the female, but life satisfaction is the opposite. With the increase in age, the individuals accumulated the highest human capital and family capital in 26–34 years old and reached the peak in life satisfaction after retirement in 60–83 years old. As far as regional differences are concerned, individual human capital, family capital, and life satisfaction are decreasing from the east to the west. The results of the study will help to establish a healthy and perfect regional urbanization and to enhance the mental health of residents by promoting talent development and advocating family-friendly construction

GPU Acceleration of Hydraulic Transient Simulations of Large-Scale Water Supply Systems

Simulating hydraulic transients in ultra-long water (oil, gas) transmission or large-scale distribution systems are time-consuming, and exploring ways to improve the simulation efficiency is an essential research direction. The parallel implementation of the method of characteristics (MOC) on graphics processing unit (GPU) chips is a promising approach for accelerating the simulations, because GPU has a great parallelization ability for massive but simple computations, and the explicit and local features of MOC meet the features of GPU quite well. In this paper, we propose and verify a GPU implementation of MOC on a single chip for more efficient simulations of hydraulic transients. Details of GPU-MOC parallel strategies are introduced, and the accuracy and efficiency of the proposed method are verified by simulating the benchmark single pipe water hammer problem. The transient processes of a large scale water distribution system and a long-distance water transmission system are simulated to investigate the computing capability of the proposed method. The results show that GPU-MOC method can achieve significant performance gains, and the speedup ratios are up to hundreds compared to the traditional method. This preliminary work demonstrates that GPU-MOC parallel computing has great prospects in practical applications with large computing load

Targeted Delivery of siRNA with pH-Responsive Hybrid Gold Nanostars for Cancer Treatment

In this work, we report the engineering of gold nanostars (GNS) to deliver small interfering RNA (siRNA) into HepG2 cells. The ligand DG-PEG-Lipoic acid (LA)-Lys-9R (hydrazone) was designed to functionalize GNS, and create the nanoparticles named as 9R/DG-GNS (hydrazone). In the ligand, 2-deoxyglucose (DG) is the targeting molecule, polyethylene glycol (PEG) helps to improve the dispersity and biocompatibility, 9-poly-d-arginine (9R) is employed to provide a positive surface charge and adsorb negative siRNA, and hydrazone bonds are pH-responsive and can avoid receptor-mediated endosomal recycling. Compared to GNS alone, 9R/DG-GNS (hydrazone) showed superior transfection efficiency. The expressions of cyclooxygenase-2 (COX-2) in HepG2 and SGC7901 cells were significantly suppressed by siRNA/9R/DG-GNS (hydrazone) complex. Notably, 9R/DG-GNS (hydrazone) possessed low cytotoxicity even at high concentrations in both normal cells and tumor cells. The combination treatment of siRNA/9R/DG-GNS (hydrazone) complex inhibited the cell growth rate by more than 75%. These results verified that the pH-responsive GNS complex is a promising siRNA delivery system for cancer therapy, and it is anticipated that near-infrared absorbing GNS with good photothermal conversion efficiency can be potentially used for photothermal therapy of tumors

Genome-wide characterization and expression profiling of NAC transcription factor genes under abiotic stresses in radish (Raphanus sativus L.)

NAC (NAM, no apical meristem; ATAF, Arabidopsis transcription activation factor and CUC, cup-shaped cotyledon) proteins are among the largest transcription factor (TF) families playing fundamental biological processes, including cell expansion and differentiation, and hormone signaling in response to biotic and abiotic stresses. In this study, 172 RsNACs comprising 17 membrane-bound members were identified from the whole radish genome. In total, 98 RsNAC genes were non-uniformly distributed across the nine radish chromosomes. In silico analysis revealed that expression patterns of several NAC genes were tissue-specific such as a preferential expression in roots and leaves. In addition, 21 representative NAC genes were selected to investigate their responses to heavy metals (HMs), salt, heat, drought and abscisic acid (ABA) stresses using real-time polymerase chain reaction (RT-qPCR). As a result, differential expressions among these genes were identified where RsNAC023 and RsNAC080 genes responded positively to all stresses except ABA, while RsNAC145 responded more actively to salt, heat and drought stresses compared with other genes. The results provides more valuable information and robust candidate genes for future functional analysis for improving abiotic stress tolerances in radish

A Spectral Shift-Based Electrochemiluminescence Sensor for Hydrogen Sulfide

Classic electrochemiluminescence (ECL) assays relying on the change in luminescence intensity face a challenge in the quantitative analysis of complex samples. Here, we report the design and implementation of a new sensing strategy, using the maximum luminescence wavelength (λ_max) shift as the readout to achieve quantitative detection. This approach includes an ECL luminophore (RuSiO₂@GO) and a H₂S-sensitive inner filter absorber (CouMC). The absorbance of CouMC illustrates a dependence on the H₂S concentration, which induces a change in the maximum luminescence wavelength (Δλ_max) of the ECL luminophore. Both experimental and simulated results suggest that the spectral shift of ECL effectively avoids the interference of the total luminescence intensity fluctuations, enabling a highly reliable quantitative analysis. This spectral shift-based ECL assay strategy offers a wide application potential by extending types of ECL luminophores and absorptive chemodosimeters, based on an inner filter effect