HEPATECTOMY IN CHILDREN: 10 YEAR EXPERIENCE IN A TERTIARY CARE HOSPITAL

Background: Hepatectomy can be safely performed in children with primary liver tumors, selected cases of metastatic tumors and traumatic liver injuries. However, liver resections are associated with significant morbidity and mortality even in advanced centers. The aim of this study was to evaluate the outcome of liver resections over a period of 10 years. Material and Methods: This was a prospective study of patients who underwent various types of hepatectomies for different indications from March 2005 to April 2015. Results: 15 patients underwent liver resections during the study period. The age of patients ranged from 8 months to 14 years with a mean age of 3.7 years. The indications in our patients were hepatoblastoma in 9 patients, traumatic liver injury in 3 patients, abscess in 1 patient, the liver extension of right Wilms tumor in 1 patient and extension of left neuroblastoma to the liver in 1 patient. Right, and left hepatectomies were done in 8 (54.4%) and 5 (33.3%) patients respectively. Nonanatomic/ wedge resection was done in 2 (13.3%) patients. Hemorrhage which needed transfusion occurred in 4 (26.7%) patients, bile leaks in 1 (6.7%), wound infection in 1 (6.7%) and death in 1 (6.7%) patients. Conclusion: Liver resection in children can be safely performed with minimal morbidity and mortality. Better results can be achieved with good per operative care, vigilant identification and good expertise in the management of complications. KEYWORDS: Hepatectomy, Children, Outcome

Kashmir-The origin of INDO-PAK Conflicts (War, Peace and Dialogue)

This is an attempt to highlight the INDO-PAK Conflicts. There are many disputes between India and Pakistan such as Water dispute over Baghlihar Dam, siachen  Glacier, Smuggling across borders, Migration etc. Apart from these disputes, there are three important issues these two countries face in their bilateral relations: 1. The differences in world views, 2. The problem over Nuclear confrontation and 3. The dispute over Kashmir The dispute over Kashmir had a major impact on both the countries-INDIA and PAKISTAN. Several times both the countries fought many wars .If this core issue is solved there will be peace and stability in the region. Keywords: Kashmir, Indo-Pak, War, Conflicts, Plebiscite

The Bleeding and Burning Kashmir: Violence, Terrorism and Freedom Struggle

Efforts have been made to discuss various issues which Kashmir Valley had faced in the past six decades –Violence, Terrorism, Aggression and Bloodshed. Political conflict in the valley have marked a high degree of militarization and the presence of herse laws giving special powers to military forces makes Kashmiri people victims of violence. This article puts a view that how India and Mainstream parties of Jammu and Kashmir had and are trying to suppress the slogans and voices Kashmiri people and Separatists that are demanding nothing but Freedom. Keywords: Freedom, Jamat-I –islami, Kashmiri pundits, Ragdo Ragdo, Violence etc

Indo- Iran relations (evolving ties from 1990-2005)

India and Iran -the great civilisations of the world had close historical and cultural ties. With the changing trends in the international politics which somehow leads towards the change in national interest had sometimes effected the relations between the two countries. However both New Delhi and Tehran are striving to maintain good and friendly relation among them to achieve their interests of national importance

Causality in String Field Theory

In this letter, we will investigate causality in string field theory using pp-wave light-cone gauge string field theory. We will generalize the Ramsey scheme to string field theory, and use it to analyze string field theoretical processes. An explicit characteristic function for interactive string field theory will be built using this string field theoretical Ramsey scheme. The average of the difference between the initial and final values of any operator described in string field theory will be obtained using this characteristic function. We will use the quantum information theoretical technique based on quantum fisher information to extract information about such string field theoretical processes.Comment: Accepted in EPL, 13 page

Development of novel functional foods using Himalayan honey having enhanced nutraceutical and nutritional potential

This study was carried out to conduct the geographical discrimination of various types of honey, and their utilization in development of novel functional foods where in honey could be substituted in place of white sugar. Honey based apple spread and marmalade products were developed and evaluated for quality analysis. The results showed higher water activity (aw) and moisture content in Plectranthus rugosus (PR) honey based apple spread and marmalade while, TSS was highest (p < 0.05) in Multifloral (MF) honey based apple spread and marmalade. Hydroxymethylfurfural was found to be in the ranged of 6.77–7.05 (mg/kg) for honey based apple spread products and 3.09–3.46 (mg/kg) for honey based apple marmalade products. The lightness (L*) value was significantly highest in MF honey based apple spread and marmalades (p < 0.05). Redness (a*) and yellowness (b*) values were significantly highest in PR honey based apple spreads and marmalades and lowest in Robinia pseudo acacia (RSA) honey based apple spread and marmalades. In general, all MF honey based apple spreads and marmalades has the highest score for overall acceptability in comparison to RSA and PR honey based apple spreads. The values of gel strength (Fe), rupture force (FR), energy of penetration (E) and adhesiveness (A) in the given spread products were in the range of 1.50–1.54 N, 1.70–1.73 N, 17.00–17.05 Ns and −1.11–−1.08 Ns, respectively. The values of gel strength (Fe), rupture force (FR), energy of penetration (E) and adhesiveness (A) in the investigated marmalade products were in the range of 1.65–1.69 N, 2.08–2.13 N, 16.05–16.10 Ns and −1.14–−1.10 Ns, respectively

Deformation of Nanowires and Nanotubes

In this article, we have investigated the consequences of the next to the leading order correction to the effective field theory of nanostructures. This has been done by analyzing the effects of deformed Heisenberg algebra on nanowires and nanotubes. We first deform the Schrodinger equation with cylindrical topology. Then specific solutions to the deformed Schrodinger equation with different boundary conditions are studied. These deformed solutions are used to investigate the consequences of the deformation on the energy of nanowires and nanotubes.Comment: 9 pages, 6 figure

Advances in Nematode Identification: A Journey from Fundamentals to Evolutionary Aspects

Nematodes are non-segmented roundworms evenly distributed with various habitats ranging to approximately every ecological extremity. These are the least studied organisms despite being the most diversified group. Nematodes are the most critical equilibrium-maintaining factors, having implications on the yield and health of plants as well as well-being of animals. However, taxonomic knowledge about nematodes is scarce. As a result of the lack of precise taxonomic features, nematode taxonomy remains uncertain. Morphology-based identification has proved inefficacious in identifying and exploring the diversity of nematodes, as there are insufficient morphological variations. Different molecular and new evolving methodologies have been employed to augment morphology-based approaches and bypass these difficulties with varying effectiveness. These identification techniques vary from molecular-based targeting DNA or protein-based targeting amino acid sequences to methods for image processing. High-throughput approaches such as next-generation sequencing have also been added to this league. These alternative approaches have helped to classify nematodes and enhanced the base for increased diversity and phylogeny of nematodes, thus helping to formulate increasingly more nematode bases for use as model organisms to study different hot topics about human well-being. Here, we discuss all the methods of nematode identification as an essential shift from classical morphometric studies to the most important modern-day and molecular approaches for their identification. Classification varies from DNA/protein-based methods to the use of new emerging methods. However, the priority of the method relies on the quality, quantity, and availability of nematode resources and down-streaming applications. This paper reviews all currently offered methods for the detection of nematodes and known/unknown and cryptic or sibling species, emphasizing modern-day methods and budding molecular techniques

Dysregulated miRNAome and Proteome of PPRV Infected Goat PBMCs Reveal a Coordinated Immune Response

In this study, the miRNAome and proteome of virulent Peste des petits ruminants virus (PPRV) infected goat peripheral blood mononuclear cells (PBMCs) were analyzed. The identified differentially expressed miRNAs (DEmiRNAs) were found to govern genes that modulate immune response based on the proteome data. The top 10 significantly enriched immune response processes were found to be governed by 98 genes. The top 10 DEmiRNAs governing these 98 genes were identified based on the number of genes governed by them. Out of these 10 DEmiRNAs, 7 were upregulated, and 3 were downregulated. These include miR-664, miR-2311, miR-2897, miR-484, miR-2440, miR-3533, miR-574, miR-210, miR-21-5p, and miR-30. miR-664 and miR-484 with proviral and antiviral activities, respectively, were upregulated in PPRV infected PBMCs. miR-210 that inhibits apoptosis was downregulated. miR-21-5p that decreases the sensitivity of cells to the antiviral activity of IFNs and miR-30b that inhibits antigen processing and presentation by primary macrophages were downregulated, indicative of a strong host response to PPRV infection. miR-21-5p was found to be inhibited on IPA upstream regulatory analysis of RNA-sequencing data. This miRNA that was also highly downregulated and was found to govern 16 immune response genes in the proteome data was selected for functional validation vis-a-vis TGFBR2 (TGF-beta receptor type-2). TGFBR2 that regulates cell differentiation and is involved in several immune response pathways was found to be governed by most of the identified immune modulating DEmiRNAs. The decreased luciferase activity in Dual Luciferase Reporter Assay indicated specific binding of miR-21-5p and miR-484 to their target thus establishing specific binding of the miRNAs to their targets.This is the first report on the miRNAome and proteome of virulent PPRV infected goat PBMCs

Comparative and temporal transcriptome analysis of peste des petits ruminants virus infected goat peripheral blood mononuclear cells

Peste des petits ruminanats virus (PPRV), a morbillivirus causes an acute, highly contagious disease – peste des petits ruminants (PPR), affecting goats and sheep. Sungri/96 vaccine strain is widely used for mass vaccination programs in India against PPR and is considered the most potent vaccine providing long-term immunity. However, occurrence of outbreaks due to emerging PPR viruses may be a challenge. In this study, the temporal dynamics of immune response in goat peripheral blood mononuclear cells (PBMCs) infected with Sungri/96 vaccine virus was investigated by transcriptome analysis. Infected goat PBMCs at 48 h and 120 h post infection revealed 2540 and 2000 differentially expressed genes (DEGs), respectively, on comparison with respective controls. Comparison of the infected samples revealed 1416 DEGs to be altered across time points. Functional analysis of DEGs reflected enrichment of TLR signaling pathways, innate immune response, inflammatory response, positive regulation of signal transduction and cytokine production. The upregulation of innate immune genes during early phase (between 2-5 days) viz. interferon regulatory factors (IRFs), tripartite motifs (TRIM) and several interferon stimulated genes (ISGs) in infected PBMCs and interactome analysis indicated induction of broad-spectrum anti-viral state. Several Transcription factors – IRF3, FOXO3 and SP1 that govern immune regulatory pathways were identified to co-regulate the DEGs. The results from this study, highlighted the involvement of both innate and adaptive immune systems with the enrichment of complement cascade observed at 120 h p.i., suggestive of a link between innate and adaptive immune response. Based on the transcriptome analysis and qRT-PCR validation, an in vitro mechanism for the induction of ISGs by IRFs in an interferon independent manner to trigger a robust immune response was predicted in PPRV infection