70,704 research outputs found
Integrative analyses of transcriptome sequencing identify novel functional lncRNAs in esophageal squamous cell carcinoma.
Long non-coding RNAs (lncRNAs) have a critical role in cancer initiation and progression, and thus may mediate oncogenic or tumor suppressing effects, as well as be a new class of cancer therapeutic targets. We performed high-throughput sequencing of RNA (RNA-seq) to investigate the expression level of lncRNAs and protein-coding genes in 30 esophageal samples, comprised of 15 esophageal squamous cell carcinoma (ESCC) samples and their 15 paired non-tumor tissues. We further developed an integrative bioinformatics method, denoted URW-LPE, to identify key functional lncRNAs that regulate expression of downstream protein-coding genes in ESCC. A number of known onco-lncRNA and many putative novel ones were effectively identified by URW-LPE. Importantly, we identified lncRNA625 as a novel regulator of ESCC cell proliferation, invasion and migration. ESCC patients with high lncRNA625 expression had significantly shorter survival time than those with low expression. LncRNA625 also showed specific prognostic value for patients with metastatic ESCC. Finally, we identified E1A-binding protein p300 (EP300) as a downstream executor of lncRNA625-induced transcriptional responses. These findings establish a catalog of novel cancer-associated functional lncRNAs, which will promote our understanding of lncRNA-mediated regulation in this malignancy
Estimation of statistical power and false discovery rate of QTL mapping methods through computer simulation
Change of rotation measure during eclipse of a black widow PSR J20510827
Black widows are millisecond pulsars ablating their companions. The material
blown from the companion blocks the radio emission, resulting in radio
eclipses. The properties of the eclipse medium are poorly understood. Here, we
present direct evidence of the existence of magnetic fields in the eclipse
medium of the black widow PSR J20510827 using observations made with the
Five-hundred-meter Aperture Spherical radio Telescope (FAST). We detect a
regular decrease in rotation measure (RM) in the egress of eclipse, changing
from to . The RM gradually
changes back to normal when the line-of-sight moves away from the eclipse. The
estimated line-of-sight magnetic field strength in the eclipse medium is G. The RM reversal could be caused by a change of the magnetic field
strength along the line of sight due to binary orbital motion. The RM reversal
phenomenon has also been observed in some repeating fast radio bursts (FRBs),
and the study of spider pulsars may provide additional information about the
origin of FRBs.Comment: 7 pages, 3 figures, accept for publication in Ap
Measurement of the chi_{c2} Polarization in psi(2S) to gamma chi_{c2}
The polarization of the chi_{c2} produced in psi(2S) decays into gamma
chi_{c2} is measured using a sample of 14*10^6 psi(2S) events collected by
BESII at the BEPC. A fit to the chi_{c2} production and decay angular
distributions in psi(2S) to gamma chi_{c2}, chi_{c2} to pi pi and KK yields
values x=A_1/A_0=2.08+/-0.44 and y=A_2/A_0=3.03 +/-0.66, with a correlation
rho=0.92 between them, where A_{0,1,2} are the chi_{c2} helicity amplitudes.
The measurement agrees with a pure E1 transition, and M2 and E3 contributions
do not differ significantly from zero.Comment: 6 pages, 4 figures, 1 tabl
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