Hypertensive intracerebral hemorrhage: Which one should we choose between laser navigation and 3D navigation mold?

BackgroundHypertensive intracerebral hemorrhage (HICH) is a severe life-threatening disease, and its incidence has gradually increased in recent years. Due to the particularity and diversity of its bleeding sites, the early treatment of hematoma needs to be more meticulous and accurate, and minimally invasive surgery is often one of the measures that are commonly adopted now. The lower hematoma debridement and the navigation template created by 3D printing technology were compared in the external drainage of a hypertensive cerebral hemorrhage. Then the effect and feasibility of the two operations were explicitly evaluated.Material and methodsWe performed a retrospective analysis of all eligible patients with HICH who underwent laser-guided hematoma evacuation or hematoma puncture under 3D-navigated molds at the Affiliated Hospital of Binzhou Medical University from January 2019 to January 2021. A total of 43 patients were treated. Twenty-three patients were treated with laser navigation-guided hematoma evacuation (group A); 20 patients were treated with 3D navigation minimally invasive surgery (group B). A comparative study was conducted between the two groups to evaluate the preoperative and postoperative conditions.ResultsThe preoperative preparation time of the laser navigation group was significantly shorter than that of the 3D printing group. The operation time of the 3D printing group was better than that of the laser navigation group (0.73 ± 0.26 h vs. 1.03 ± 0.27 h P = 0.00070). In the improvement in the short-term postoperatively, there was no statistically significant difference between the laser navigation group and the 3D printing group (Median hematoma evacuation rate P = 0.14); And in the three-month follow-up NIHESS score, there was no significant difference between the two (P = 0.82).ConclusionLaser-guided hematoma removal is more suitable for emergency operations, with real-time navigation and shortened preoperative preparation time; hematoma puncture under a 3D navigation mold is more personalized and shortens the intraoperative time course. There was no significant difference in therapeutic effect between the two groups

Photoperiod-responsive changes in chromatin accessibility in phloem companion and epidermis cells of Arabidopsis leaves

Photoperiod plays a key role in controlling the phase transition from vegetative to reproductive growth in flowering plants. Leaves are the major organs perceiving day-length signals, but how specific leaf cell types respond to photoperiod remains unknown. We integrated photoperiod-responsive chromatin accessibility and transcriptome data in leaf epidermis and vascular companion cells of Arabidopsis thaliana by combining isolation of nuclei tagged in specific cell/tissue types with assay for transposase-accessible chromatin using sequencing and RNA-sequencing. Despite a large overlap, vasculature and epidermis cells responded differently. Long-day predominantly induced accessible chromatin regions (ACRs); in the vasculature, more ACRs were induced and these were located at more distal gene regions, compared with the epidermis. Vascular ACRs induced by long days were highly enriched in binding sites for flowering-related transcription factors. Among the highly ranked genes (based on chromatin and expression signatures in the vasculature), we identified TREHALOSE-PHOSPHATASE/SYNTHASE 9 (TPS9) as a flowering activator, as shown by the late flowering phenotypes of T-DNA insertion mutants and transgenic lines with phloem-specific knockdown of TPS9. Our cell-type-specific analysis sheds light on how the long-day photoperiod stimulus impacts chromatin accessibility in a tissue-specific manner to regulate plant development

Three-dimensional laser combined with C-arm computed tomography-assisted puncture of intracerebral hemorrhage

BackgroundIntracerebral hemorrhage (ICH) is the deadliest subtype of stroke, with a 30-day case fatality rate of approximately 40%. Timely and accurate treatment is essential to facilitate recovery. The introduction of stereotactic instruments and navigation systems has greatly improved the accuracy of surgical treatment. In this study, we explored the application and effects of a three-dimensional (3D) laser combined with C-arm computed tomography (CT) on ICH puncture.Materials and methodsAccording to the principle of randomness, 118 patients with ICH were divided into control and experimental groups. The control group was treated with CT-guided puncture, and the experimental group was treated with 3D laser combined with C-arm CT puncture. The hematoma clearance rates at 3, 5, and 7 days after surgery and the prognosis at 1, 3, and 6 months after surgery were compared between the two groups.ResultsThe hematoma clearance rates of the group using 3D laser combined with C-arm CT at 3, 5, and 7 days after surgery were significantly higher than those of the control group, and the difference was statistically significant (p < 0.05). One month postoperatively, the daily living ability (ADL) grading and recovery of the patients in the test group was significantly better than those of the control group (p < 0.05), but there was no statistically significant difference in ADL 3 and 6 months after surgery (p > 0.05).Conclusion3D laser combined with C-arm CT puncture has the advantages of real-time guidance, accurate positioning, and simple operation. It is an effective minimally invasive surgical method that is easy to master

Comparative genomics reveals the hybrid origin of a macaque group

Although species can arise through hybridization, compelling evidence for hybrid speciation has been reported only rarely in animals. Here, we present phylogenomic analyses on genomes from 12 macaque species and show that the fascicularis group originated from an ancient hybridization between the sinica and silenus groups ~3.45 to 3.56 million years ago. The X chromosomes and low-recombination regions exhibited equal contributions from each parental lineage, suggesting that they were less affected by subsequent backcrossing and hence could have played an important role in maintaining hybrid integrity. We identified many reproduction-associated genes that could have contributed to the development of the mixed sexual phenotypes characteristic of the fascicularis group. The phylogeny within the silenus group was also resolved, and functional experimentation confirmed that all extant Western silenus species are susceptible to HIV-1 infection. Our study provides novel insights into macaque evolution and reveals a hybrid speciation event that has occurred only very rarely in primates

The complete chloroplast genome of Torreya parvifolia, a species with extremely small population in China

Torreya parvifolia (Torreya, Taxaceae) is endemic in Sichuan, China. It consisted of an extremely small population with less than 100 wild individuals. In this study, the complete chloroplast genome of T. parvifolia was assembled using the Illumina data. The complete chloroplast genome of T. parvifolia is 137,106 bp in length. The genome consists of 119 genes in total, including 82 protein-coding genes (PCGs), 4 ribosomal RNA (rRNA) genes, and 33 transfer RNA (tRNA) genes. Phylogenetic analysis indicated that T. parvifolia was closely related to T. fargesii, T. nucifera, and T. fargesii var. yunnanensis with strong support

Soil respiration responses to soil physiochemical properties in urban different green-lands: A case study in Hefei, China

Soil respiration (RS) is an important carbon budget in urban ecosystem. In order to better understand the limiting factors affecting urban soil respiration, we measured RS, soil temperature, soil moisture content, soil organic carbon (SOC), nitrogen (N), C/N, dissolved organic carbon (DOC), microbial biomass carbon (MBC), NO3−-N, NH4+-N, P and fine root biomass from twelve sites of four green-land types (campus green-land, park green-land, residential green-land and factory green-land) for two years in built-up areas of Hefei, China. The results showed that average annual RS was significantly lower in the residential green-land (1.35 μmol m−2 s−1) than in the campus (2.64 μmol m−2 s−1) and park (2.51 μmol m−2 s−1) green-lands. RS positively increased with soil temperature at the range of 2.01–31.26 °C, and Q10 values ranged from 1.48 to 1.65 in the four types of green-lands. Soil moisture (18–25%) showed significantly positive correlation with soil respiration (P<0.01). When precipitation occurred frequently in wet summer, soil moisture served as the dominant control on RS variations. RS was positively related with SOC, NO3−-N, P and fine root biomass (diameter <2 mm), while negatively correlated with DOC at 0–10 cm depth. Our results indicate that decreasing RS may be an optional way to increase carbon sequestration potential for urban ecosystem, and this can be achieved by regulating green-land types and applying appropriate soil nutrients maintenance practices

Properties and low-temperature performance of biomass heavy oil used in road applications

Biomass heavy oil, a renewable green energy source, has been extensively explored for its preparation process and material properties. This study examined the applicability of biomass heavy oil integrated into matrix asphalt. Bio-asphalt samples were prepared using polymer-modified asphalt I-C as the matrix asphalt, blended with straw liquid oil, straw solid oil, and castor liquid oil. The low-temperature performances of these samples were assessed. First, the effects of bio-oil on the fundamental performance of asphalt were analyzed through three-component tests, encompassing a short-term aging test and a temperature scanning test based on dynamic shear rheometry. Subsequently, the effects of the chemical composition of bio-asphalt on its fundamental performance were examined through four-component tests, including SARA, gel-permeation chromatography (GPC), and gray entropy correlation. The glass transition temperature (Tg) was considered a parameter for evaluating the low-temperature properties of the bio-asphalt binders. The bending-beam rheometry (BBR) low-temperature creep test and the binder fracture energy (BFE) full-section fracture energy test revealed that adding bio-oil reduces Tg of the matrix asphalt. The integrated creep flexural parameter Jc and the BFE fracture energy density values indicated that the low-temperature fracture-resistant properties of bio-asphalt surpass those of the matrix asphalt. Furthermore, the low-temperature cracking resistance of the bio-asphalt samples was determined through the BFE test. Gray entropy correlation analysis of the low-temperature performance indicators of bio-asphalt revealed that the BBR test is suitable for evaluating low-temperature performance only up to − 12 °C. In summary, the bio-asphalt mixtures demonstrate exceptional fatigue resistance under low-temperature service conditions. Therefore, bio-oil emerges as an effective candidate for enhancing the low-temperature performance characteristics of matrix asphalt

Bi-Directional Cuk Equalizer-Based Li-Ion Battery Pack Equalization Control Strategy Research

For the secure usage of battery charging and discharging within electric vehicles, the study of cell pack equalization technology is essential. Therefore, in this paper, an improved Bidirectional Cuk equalizer (BCEQ) structure based on a variable-domain fuzzy PID (VFPID) control equalization strategy is recommended in stages. With the new equalization topology, only half of the capacitive and inductive components are needed to transfer energy between any two individual cells in the power supply. In addition, the proposed VFPID control strategy further improves the efficiency of the equalization model by dynamically adjusting the magnitude of the equalization current parameters. Through simulation experiments, the improved topology was capable of substantially lessening the equalization time and increasing energy utilization by more than 4%. In comparison with the fuzzy PID (FPID) algorithm, around 27.3% faster equalization times can be achieved with the VFPID algorithm; the VFPID algorithm also performed well under the Dynamic Stress Test (DST) condition, demonstrating that the topology and equalization strategy suggested in this paper can successfully address the inconsistency of the FPID algorithm. It has been revealed that the topology and equalization methodology offered in this paper is effective in solving the battery pack inconsistency