Matrix Tensor Product Approach to the Equivalence of Multipartite States under Local Unitary Transformations

The equivalence of multipartite quantum mixed states under local unitary transformations is studied. A criterion for the equivalence of non-degenerate mixed multipartite quantum states under local unitary transformations is presented.Comment: 7 page

Gender-specific association of MSA2756G with hypertension in patients attending a health facility in Ningxia Province, China

Purpose: To investigate the distribution of methionine synthase A2756G (MSA2756G) in the hypertensive patients in northwest Chinese population.Methods: A total of 378 unrelated hypertensive patients attending Ningxia Peoples Hospital, Ningxia Province, China, were recruited for this study. We analyzed genotype by amplication - created restriction sites (ACRS) and polymerase chain reaction - restrict fragment length polymorphism (PCR - RFLP) in hypertensive patients, and inspected the relation of the genotype with hypertension by χ2 and t test.Results: The frequency of G allele was 10.25 % in the control group and 14.04 % in hypertension group; it was not statistically different (p > 0.05). In the male group, the frequency of allele G was 11.50 % in control group, and 8.79 % in hypertension group. There was no significant difference between control and hypertension groups (p > 0.05). In the female group, the frequency of allele G was 9.00 %, in control and 19.54 % in hypertension group (p < 0.05), while in the hypertension group, allele G was 8.79 % in males which is significantly lower (p < 0.05) than in females (19.54 %) .Conclusion: Allele G of MSA2756G is a risk factor for hypertension in female in this Chinese population of this study.Keywords: Hypertension, Methionine synthase, Polymorphism, Gender, Amplification-created restriction sites, Allele G, MSA2756

慢型克山病与扩张型心肌病患者抗氧化能力对比研究

Objective: To compare the serum levels of selenium, selenoprotein and malondialdehyde about Keshan disease (KSD) and dilated cardiomyopathy (DCM) patients and to investigate the relationship between the antioxidant capacity and myocardial injury. Methods: 26 KSD patients, 18 DCM patients, 24 healthy people in KSD areas and 22 healthy people in non-KSD areas were selected by all-round physical examination and electrocardiography. Blood samples were collected by informing consent and volunteering. The serum selenium was determined by using 2,3 - two amino-naphthalene fluorescence method. The serum selenoprotein and malondialdehyde were measured by using biotin double-antibody sandwich method of enzyme linked immunosorbent assay (ELISA) (according to kit instructions). Results: There were no statistical differences about the distribution of the gender and age in every subjects’ groups (P＞0.05). The levels of serum selenium and selenoprotein in Keshan disease group and dilated cardiomyopathy group were significantly lower than those of healthy group whether in KSD areas or in non- KSD areas , and the serum malondialdehyde were higher than those in healthy controls. There were no statistical significance about the concentrations of serum selenium, selenoprotein and malondialdehyde between Keshan disease group and dilated cardiomyopathy group, KSD areas’ control group and non- KSD areas’ control group. Conclusion: The myocardial injury of KSD patients and dilated cardiomyopathy patients may be associated with the decrease of antioxidant ability because of the lower selenium levels.目的  对比研究慢型克山病与扩张型心肌病患者体内血清硒、硒蛋白和丙二醛浓度，探讨抗氧化能力与心肌损伤的关系。方法  选择慢型克山病患者26例，扩张型心肌病患者18例，同时在克山病病区选取24名经过全面查体和心电图描记无异常的健康人作为病区健康对照组，在非克山病区选择22名健康人作为非病区健康对照组。在知情同意、患者自愿情况下采集受检者血样，检测克山病患者、扩心病患者、病区健康人及非病区健康人的血清硒、硒蛋白水平及丙二醛浓度。血清硒含量测定采用2,3-二氨基萘荧光法；血清硒蛋白和丙二醛浓度测定采用生物素双抗体夹心酶联免疫吸附法（ELISA）（按试剂盒说明操作）。结果  各组调查对象性别及年龄分布均无统计学差异（P＞0.05）；克山病组和扩张型心肌病组血清硒、硒蛋白水平均明显低于病区健康对照组与非病区健康对照组，血清丙二醛浓度均高于病区健康对照组及非病区健康对照组；克山病组与扩张型心肌病组，病区健康对照组与非病区健康对照组血清硒、硒蛋白水平以及丙二醛浓度差异均无显著性。结论  慢型克山病和扩张型心肌病患者心肌损伤都可能与低硒导致的抗氧化能力降低有关

Expressions and clinic significance of miRNA-143, miRNA- 34A, miRNA-944, miRNA-101 and miRNA-218 in cervical cancer tissues

Purpose: To search for novel biomarkers for early diagnosis of cervical cancer, as well as novel therapeutic target for cervical cancer.Methods: A total of 96 cervical tissue specimens were collected from patients in the Second Affiliated Hospital of Zhengzhou University, out of which 10 were normal control. The remaining specimens (86) were cervical cancer specimens and were divided into 4 groups (A - D) based on tumor-biomarker levels of CA125 and SCC. Quantitative real-time polymerase chain reaction technology (qRT-PCR) was used to detect the expressions of miRNA-143, miRNA-34A, miRNA-944, miRNA-101 and miRNA-218 in the cervical cancer tissues.Results: The levels of CA125 (U/mL) and SCC (ug/L) expressed in normal control group and groups A - D were 11.75 and 0.73 (n = 10), 382 and 2.72 (n = 25), 912.9 and 3.93 (n = 21), 1675 and 5.87 (n = 29), and 2120 and 6.66 (n = 11), respectively. Furthermore, qRT-PCR results showed that the expressions of miRNA-944 and miRNA-218 in cervical cancer tissues were markedly up-regulated compared to normal control tissues (p < 0.01). In contrast, the expression level of miRNA-143, miRNA-34A, and miRNA-101 were significantly decreased (p < 0.01).Conclusion: The biomarkers, miRNA-143, miRNA-34A, miRNA-944, miRNA-101 and miRNA-218, can be considered novel for early diagnosis of cervical cancer.Keywords: Cervical cancer, Biomarkers, miRNA-143, miRNA-34A, miRNA-944, miRNA-101, miRNA- 21

Gene expression profile analysis of tobacco leaf trichomes

<p>Abstract</p> <p>Background</p> <p>Leaf trichomes of <it>Nicotiana tabacum </it>are distinguished by their large size, high density, and superior secretion ability. They contribute to plant defense response against biotic and abiotic stress, and also influence leaf aroma and smoke flavor. However, there is limited genomic information about trichomes of this non-model plant species.</p> <p>Results</p> <p>We have characterized <it>Nicotiana tabacum </it>leaf trichome gene expression using two approaches. In the first, a trichome cDNA library was randomly sequenced, and 2831 unique genes were obtained. The most highly abundant transcript was ribulose bisphosphate carboxylase (RuBisCO). Among the related sequences, most encoded enzymes involved in primary metabolism. Secondary metabolism related genes, such as isoprenoid and flavonoid biosynthesis-related, were also identified. In the second approach, a cDNA microarray prepared from these 2831 clones was used to compare gene expression levels in trichome and leaf. There were 438 differentially expressed genes between trichome and leaves-minus-trichomes. Of these, 207 highly expressed genes in tobacco trichomes were enriched in second metabolic processes, defense responses, and the metabolism regulation categories. The expression of selected unigenes was confirmed by semi-quantitative RT-PCR analysis, some of which were specifically expressed in trichomes.</p> <p>Conclusion</p> <p>The expression feature of leaf trichomes in <it>Nicotiana tabacum </it>indicates their metabolic activity and potential importance in stress resistance. Sequences predominantly expressed in trichomes will facilitate gene-mining and metabolism control of plant trichome.</p

Precise and label-free tumour cell recognition based on a black phosphorus nanoquenching platform

Breast cancer is a type of heterogeneous disease, which manifests as different molecular subtypes due to the complex nature of tumour initiation, progression, and metastasis. Accurate identification of a breast cancer subtype plays crucial roles in breast cancer management. Herein, taking advantage of the efficient quenching properties of black phosphorus nanosheets (BPNSs), in combination with the high specificity of ssDNA (or RNA) aptamer, a fluorometric duplexed assay that is capable of the simultaneous detection of two tumour markers within one run is developed. When mixed with BPNSs, the fluorescence of both FAM and Cy3 labelled aptamers was quenched. The presence of different subtypes of breast cancer cells restored the FAM and Cy3 fluorescence in distinct patterns according to their intrinsic features. The proposed assay can precisely recognise label-free breast cancer subtypes, providing an efficient method for cell type identification and guidance for subsequent breast cancer treatment. The significance of the proposed study is two-fold. First, we provide a simple method for sensitive and specific tumour cell detection; secondly, and more importantly, the proposed dual assay allows precise recognition of tumour cells and thus opens a door for rapid characterization and sorting of a wide range of tumours without using expensive instruments

Separability of rank two quantum states on multiple quantum spaces

Explicit sufficient and necessary conditions for separability of $N$-dimensional rank two multiparty quantum mixed states are presented. A nonseparability inequality is also given, for the case where one of the eigenvectors corresponding to nonzero eigenvalues of the density matrix is maximally entangled.Comment: 10 pages, Late