Effects of dietary supplementation of Chinese herb medicine mixture on rumen fermentation, nutrient digestion and blood profile in goats

Twenty adult goats, fitted with ruminal cannulae, were used to investigate the effects of dietary supplementation of Chinese herbal medicine (CHM) mixtures on rumen fermentation, nutrient digestion and blood profiles. The wethers were assigned equally to one of the two dietary treatments according to a randomized controlled trial design. One dietary treatment was the basal diet, while the other was the CHM mixture diet consisting of the basal diet and the CHM mixture. Chinese Mosla, Atractylodes rhizome, Officinal Magnolia bark, white hyacinth bean and Liriope root tuber were mixed with a weight ratio of 8:4:4:4:5 to make up the CHM mixture. This mixture was added to the basal diet at 15 g/kg feed. The dietary supplementation of the mixture increased ruminal fluid concentrations of acetate, butyrate and total volatile fatty acids, and the ruminal activities of avicelase, carboxymethyl cellulase, cellobiase and xylanase. It also improved the ruminal effective degradability of dry matter, organic matter and amylase-treated ash-free neutral detergent fibre from the forage, apparent total tract digestibility of gross energy, dry matter, organic matter, crude protein, amylase-treated ash-free neutral detergent fibre, ash-free acid detergent fibre and calcium. Whole blood pH and concentrations of actual bicarbonate, buffer excess and magnesium ion, plasma concentrations of leptin, total protein, albumin, globulin, C-reactive protein, immunoglobulin A/G/M and complement 3/4 increased. Furthermore, plasma activities of nitric oxide synthase, glutathione peroxidase and total superoxide dismutase and plasma total antioxidative capacity increased. However, plasma concentrations of adrenocorticotropic hormone, aldosterone, cortisol, glucose, urea nitrogen and lactic acid and plasma activities of alanine transaminase, creatine kinase and lactate dehydrogenase decreased. These results indicated that the dietary supplementation of the CHM mixture exerted some positive regulating effects on the nervous system, the endocrine system and the immune system of goats, thus improving the functions of immunity and antioxidation, as well as the digestion and metabolism of nutrients. However, the CHM mixture did not affect their rumen fermentation patterns.Keywords: Chinese herbal medicine, rumen environmental parameter, digestibility, blood indicator, ruminan

Use of Clinpro Cario in diagnosing childhood caries

Abstract no. 0700published_or_final_versio

Pseudomonas sp. strain WJ04 enhances current generation of Synechocystis sp. PCC6803 in photomicrobial fuel cells

Photomicrobial fuel cells (pMFCs) are a new technology for transforming the light energy or bioenergy of algae into bioelectricity. However, the current methods of generating algal pMFCs have poor efficiency due to the complex electron transfer network in algal cells. In this study, pMFCs containing the model electricity-producing cyanobacterium Synechocystis sp. PCC6803 contaminated by additional bacteria exhibited a 12.6-fold greater voltage output compared to the control pMFCs with axenic Synechocystis sp. PCC6803. To explain this phenomenon, the compositions of symbiotic bacterial communities in bacterial-contaminated pMFCs were analyzed, and a pure strain WJ04 belonging to the genus Pseudomonas, which was the second most abundant (30.2%) among the total symbiotic bacteria, was obtained. The strain WJ04 was able to raise the voltage output by 8.3 fold compared with the control treatments, meaning that strain WJ04 contributed to the increased voltage output of pMFCs. Through the coculture experiment, strain WJ04 was found to influence the growth of Synechocystis, improve photosynthesis rates by regulating algal genes and further affect algal metabolite production compared with control treatments. In addition, nicotinamide, c-homocysteic acid and 4-aminobutyric acid (fold change analysis, F.C. &gt;= 10) could regulate algal gene expression and enhance the coculture current density by 6.1-12.3 fold compared with the control treatments. Thus, these results provide a clear explanation for the high current production in bacterial-contaminated pMFCs, clarify the interaction mechanisms between algae and bacteria in the anode and provide accurate evidence for the role of bacteria in improving algal bioelectricity utilization.</p

An Integrated Road Construction and Resource Planning Approach to the Evacuation of Victims From Single Source to Multiple Destinations

This paper presents our study on the emergency resource-planning problem, particularly on the development of a new approach to resource planning through contraflow techniques with consideration of the repair of damaged infrastructures. The contraflow technique is aimed at reversing traffic flows in one or more inbound lanes of a divided highway for the outbound direction. As opposed to the current literature, our approach has the following salient points: (1) simultaneous consideration of contraflow and repair of repair of roads; (2) classification of victims in terms of their problems and urgency in sending them to a safe place or place to be treated; and (3) consideration of multiple destinations for victims. A simulated experiment is also described by comparing our approach with some variations of our approach. The experimental results show that our approach can lead to a reduction in evacuation time by more than 50%, as opposed to the original resource operation on the damaged transportation network, and by about 20%, as opposed to the approach with resource replanning (only) on the damaged network. In addition, the multiobjective optimization algorithm to solve our model can be generalized to other network resource-planning problems under infrastructure damage

Evacuation Planning Based on the Contraflow Technique With Consideration of Evacuation Priorities and Traffic Setup Time

Evacuation planning with the contraflow technique is a complex planning problem. The problem is further complicated when more realistic situations such as evacuation priorities and the setup time for the contraflow operation are considered. Such a complex problem has yet to be discussed in the present literature. In this paper, we present a multipleobjective optimization model for this problem and a two-layer algorithm to solve this model. Experiments on three transportation networks with different network scales are presented to show the excellent performance of the proposed model and algorithm.published_or_final_versio

Thermal evolution history after collision of North China plate with Yangtze plate

对采自苏北一胶南一大别高压变质构造混杂岩带的片麻岩、糜棱岩和郑庐断裂带上的片麻岩中9个钾长石进行了40Ar-39Ar 年龄测定和多重扩散域(MDD)模式处理, 9个样品的热演化史表明上述地区存在5个不同的快速冷却时段, 并就其可能的构造含义, 提出了华北与扬子板块碰撞后的折返历史过程。40Ar-39Ar analyses and MDD（multiple diffusion domain）model treatements were performed for 9 K-feldspar samples. They were collected from gneiss and mylonite of North Jiangsu-Jiaonan-Dabie tectonic melange belt and Tancheng-Lujiang fault zone. The thermal evolution history exhibits five fast ccoling stages found in these samples．In relation with their possible tectonic implications a recovery process after the collision of the North China plate with the Yangtze plate is suggested here.published_or_final_versio

Analytical technology aided optimization and scale-up of impinging jet mixer for reactive crystallization process

Reactive crystallization is widely used in the manufacture of active pharmaceutical ingredients (APIs). Since APIs often have low solubility, traditional stirred tank reactors and the route of process operation and control using metastable zone width are not effective. The current work investigated the integration of an impinging jet mixer and a stirred tank crystallizer that can take advantage of both the reaction and crystallization characteristics, the focus being on design optimization and scale-up using process analytical techniques based on the Fourier transform Infrared spectroscopy and Focused Beam Reflectance Measurement, as well as X-ray diffraction and particle imaging Morphologi G3. The parameters for process operation and design of the impinging jet mixer were optimized. The research was carried out with reference to the manufacture of an antibiotic, sodium cefuroxime, firstly in a 1L reactor, then a 10L reactor. The crystals produced showed higher crystallinity, narrower size distribution, higher stability and purity

Promoter hypermethylation and histone hypoacetylation contribute to pancreatic-duodenal homeobox 1 silencing in gastric cancer

Background and Aims: The expression of pancreatic-duodenal homeobox 1 (PDX1) in gastric cancer is aberrantly reduced. The aim of this study was to elucidate the regulation of DNA methylation and histone acetylation at the promoter for PDX1 silencing in gastric cancer. Methods: PDX1 expression in response to demethylation and acetylation was detected in human gastric cancer cell lines by reverse transcription-polymerase chain reaction (PCR) and western blot. Four CpG islands within the 5#-flanking region of PDX1 gene were analyzed with their transcription activities being detected by dual luciferase assay. Promoter hypermethylation was identified in gastric cancer cell lines and cancer tissues by methylation-specific PCR or bisulfite DNA sequencing PCR analysis. Histone acetylation was determined by chromatin immunoprecipitation (ChIP) assay. Results: Demethylation by 5′-aza-2′-deoxycytidine (5′-aza-dC) and/or acetylation by trichostatin A (TSA) restored PDX1 expression in gastric cancer cells. Hypermethylation was found in four CpG islands in six of seven cancer cell lines. However, only the distal CpG island located in the promoter fragment of PDX1, F383 (c.22063 to 21681 nt upstream of the ATG start codon) displayed significant transcriptional activity that could be suppressed by SssI methylase and increased by 5′-aza-dC and TSA. More than 70% of the single CpG sites in F383 were methylated with hypermethylation of F383 fragment more common in gastric cancerous tissues compared with the paired normal tissues (P < 0.05). ChIP assay showed F383 was also associated with low hypoacetylation level of the histones. Conclusion: Promoter hypermethylation and histone hypoacetylation contribute to PDX1 silencing in gastric cancer. © The Author 2010. Published by Oxford University Press. All rights reserved.postprin

Modeling the stress dependence of magnetic hysteresis based on Stoner-Wohlfarth theory

© 2015 IEEE. This paper presents an improved approach for simulating magnetic hysteresis, which takes into account the effect of applied stress, based on an extended Stoner-Wohlfarth (S-W) model. Meanwhile, the S-W asteroid rotates and shrinks, and the stable direction of magnetization of the particle can be calculated from the new energy minimum conditions. This developed model is applied to analyze the magnetic hysteresis phenomenon of a soft magnetic composite (SMC) material under different compaction process, and the results of simulations are in good quantitative agreement with experimental data