Mating evolution in Gambusia (Poeciliidae) : an integration of behavior, molecules and morphology

textFemale mate choice and male courtship display are critical behaviors for the understanding of character evolution driven by sexual selection. This thesis is designated to understand the evolutionary mechanism of these two behaviors with mosquito fish (Gambusia). In the first chapter, collaborated with Dr. Mark Kirkpatrick, we demonstrated positive coevolution of courtship display and morphological signatures of male coercion and male advantage in sexually antagonistic adaptation across 10 Gambusia species. This finding suggested that male display may have caused the evolution of morphologies involved in SAC, or conversely it may have evolved as a palliative byproduct of the morphologies. This unexpected observation raised new interpretation about evolutionary cause and consequence of displays across different mating systems. The second chapter examined whether neuromolecular underpinning of G. affinis female mate choice is canalized or plastic in mating systems that show variable extant of mate choice. With Dr. Mary Ramsey, we should positive correlations between gene expression and female preference strength during exposure to courting heterospecific males, but a reversed pattern following exposure to coercive heterospecific males. This suggested that the neuromolecular entities associated with female preference are plastic and responsive to different male phenotypes (courting or coercive) rather than a canalized response linked to mating system. Further, I proposed that female behavioral plasticity may involve learning because female association patterns shifted with experience/age. Compared to younger females, I find that more experienced females spend less time near coercive males but associate more with males in the presence of courters. We thus suggested a conserved learning-based neuromolecular process underlying the diversity of female mate preference across the mate choice and coercion-driven mating systems.Ecology, Evolution and Behavio

Frugal Optimization for Cost-related Hyperparameters

The increasing demand for democratizing machine learning algorithms calls for hyperparameter optimization (HPO) solutions at low cost. Many machine learning algorithms have hyperparameters which can cause a large variation in the training cost. But this effect is largely ignored in existing HPO methods, which are incapable to properly control cost during the optimization process. To address this problem, we develop a new cost-frugal HPO solution. The core of our solution is a simple but new randomized direct-search method, for which we prove a convergence rate of $O(\frac{\sqrt{d}}{\sqrt{K}})$ and an $O(d\epsilon^{-2})$-approximation guarantee on the total cost. We provide strong empirical results in comparison with state-of-the-art HPO methods on large AutoML benchmarks.Comment: 29 pages (including supplementary appendix

A New Method of PV Array Faults Diagnosis in Smart Grid

A new fault diagnosis method is proposed for PV arrays with SP connection in this study, the advantages of which are that it would minimize the number of sensors needed and that the accuracy and anti-interference ability are improved with the introduction of fuzzy group decision-making theory. We considered five “decision makers” contributing to the diagnosis of PV array faults, including voltage, current, environmental temperature, panel temperature, and solar illumination. The accuracy and reliability of the proposed method were verified experimentally, and the possible factors contributing to diagnosis deviation were analyzed, based on which solutions were suggested to reduce or eliminate errors in aspects of hardware and software

The Detection and Phylogenetic Analysis of Bovine Hepacivirus in China

Hepacivirus has been identified in cattle in Africa, Europe, and South America. In this survey of bovine hepacivirus (BovHepV) in 131 serum samples from Chinese cattle herds using RT-PCR, five of 131 sera were BovHepV positive, with the infection rate of 3.82%. Phylogenetic analysis based on the partial NS3 coding sequence showed that the BovHepV of the five positive samples clustered with other BovHepV but formed a separate branch. The results indicated that these new BovHepV represent emerging and novel strains. Further investigations are necessary to determine the epidemiology and viral pathogenesis of these BovHepV strains, as well as the potential threat to ruminant and livestock workers in China

Ginsenoside Rh1 Improves the Effect of Dexamethasone on Autoantibodies Production and Lymphoproliferation in MRL/lpr Mice

Ginsenoside Rh1 is able to upregulate glucocorticoid receptor (GR) level, suggesting Rh1 may improve glucocorticoid efficacy in hormone-dependent diseases. Therefore, we investigated whether Rh1 could enhance the effect of dexamethasone (Dex) in the treatment of MRL/lpr mice. MRL/lpr mice were treated with vehicle, Dex, Rh1, or Dex + Rh1 for 4 weeks. Dex significantly reduced the proteinuria and anti-dsDNA and anti-ANA autoantibodies. The levels of proteinuria and anti-dsDNA and anti-ANA autoantibodies were further decreased in Dex + Rh1 group. Dex, Rh1, or Dex + Rh1 did not alter the proportion of CD4+ splenic lymphocytes, whereas the proportion of CD8+ splenic lymphocytes was significantly increased in Dex and Dex + Rh1 groups. Dex + Rh1 significantly decreased the ratio of CD4+/CD8+ splenic lymphocytes compared with control. Con A-induced CD4+ splenic lymphocytes proliferation was increased in Dex-treated mice and was inhibited in Dex + Rh1-treated mice. Th1 cytokine IFN-γ mRNA was suppressed and Th2 cytokine IL-4 mRNA was increased by Dex. The effect of Dex on IFN-γ and IL-4 mRNA was enhanced by Rh1. In conclusion, our data suggest that Rh1 may enhance the effect of Dex in the treatment of MRL/lpr mice through regulating CD4+ T cells activation and Th1/Th2 balance

Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients

Cancer driven by somatic mutations may express neoantigens that can trigger T-cell immune responses. Since T-cell receptor (TCR) repertoires play critical roles in anti-tumor immune responses for oncology, next-generation sequencing (NGS) was used to profile the hypervariable complementarity-determining region 3 (CDR3) of the TCR-beta chain in peripheral blood samples from 68 gastric cancer patients and 49 healthy controls. We found that most hyper-expanded CDR3 are individual-specific, and the gene usage of TRBV3-1 is more frequent in the tumor group regardless of tumor stage than in the healthy control group. We identified 374 hyper-expanded tumor-specific CDR3, which may play a vital role in anti-tumor immune responses. The patients with stage IV gastric cancer have higher EBV-specific CDR3 abundance than the control. In conclusion, analysis of the peripheral blood TCR repertoires may provide the biomarker for gastric cancer prognosis and guide future immunotherapy

Liquid–Liquid Equilibria of Ionic Liquids–Water–Acetic Acid Mixtures

International audienceThe liquid–liquid equilibria of ionic liquid-based systems with water and/or acetic acid have been studied at 293.15 K and atmospheric pressure. One hydrophilic ionic liquid and a series of hydrophobic ionic liquids were investigated in order to examine their effect on the separation of water and acetic acid mixtures. The ionic liquids studied were [P666,14]Cl, [P666,14][NTf2], [C4mmim][NTf2], [Cnmim][NTf2] (n = 2, 4, 6, 8, or 10), [C4mpyrr][NTf2], [N1114][NTf2], and [C2mim][EtSO4]. [C2mim][EtSO4] is totally miscible with water and acetic acid in all compositions. Comparing [P666,14]Cl with [P666,14][NTf2], the former showed higher extraction selectivities; however, due to the larger viscosity of [P666,14]Cl, the [NTf2]− based ionic liquids offer a better solvent choice for the liquid extraction processes. As expected, as the solubility of water decreases with increasing the chain length of ionic liquids, this in turn leads to [C10mim][NTf2] showing greater acetic efficiency than [C2mim][NTf2] for the separation of water and acetic acid. The experimental data obtained for ternary systems containing the [C4mmim][NTf2] demonstrated that the modification of the C(2) position on the imidazolium ring does not significantly affect the selectivity compared with [C4mim][NTf2]. Tetraalkyl ammonium and N-alkyl pyrrolidinium based ionic liquids were also studied with the [NTf2]− anion with the results for the system containing the [C4mpyrr][NTf2] demonstrating a higher selectivity for the separation of water and acetic acid than the other [NTf2]− based systems studied. All experimental data were then correlated using the UNIQUAC model within an accuracy close to 1.6%. Finally, the ionic liquids were also compared with standard molecular extraction solvent, for example, methyl tert-butyl ether and methyl isobutyl ketone. The organic solvents showed an advantage over the [Cnmim][NTf2]-based ionic liquids but only over a narrow composition range. In all ionic liquid systems, the selectivity remains high at low acetic acid concentration compared with that found in the organic solvents, which is important for practical operation and demonstrates the advantages of using an ionic liquid for the extraction