Correction of image radial distortion based on division model

This paper presents an approach for estimating and then removing image radial distortion. It works on a single image and does not require a special calibration. The approach is extremely useful in many applications, particularly those where human-made environments contain abundant lines. A division model is applied, in which a straight line in the distorted image is treated as a circular arc. Levenberg–Marquardt (LM) iterative nonlinear least squares method is adopted to calculate the arc’s parameters. Then “Taubin fit” is applied to obtain the initial guess of the arc’s parameters which works as the initial input to the LM iteration. This dramatically improves the convergence rate in the LM process to obtain the required parameters for correcting image radial distortion. Hough entropy, as a measure, has achieved the quantitative evaluation of the estimated distortion based on the probability distribution in one-dimensional θ Hough space. The experimental results on both synthetic and real images have demonstrated that the proposed method can robustly estimate and then remove image radial distortion with high accurac

Panoramic mosaics from Chang’E-3 PCAM images at Point A

This paper presents a unique approach for panoramic mosaics based on Moon surface images from the Chang’E-3 (CE-3) mission, with consideration of the exposure time and external illumination changes in CE-3 Panoramic Camera (PCAM) imaging. The engineering implementation involves algorithms of image feature points extraction by using Speed-Up Robust Features (SURF), and a newly defined measure is used to obtain the corresponding points in feature matching. Then, the transformation matrix is calculated and optimized between adjacent images by the Levenberg–Marquardt algorithm. Finally, an image is reconstructed by using a fade-in-fade-out method based on linear interpolation to achieve a seamless mosaic. The developed algorithm has been tested with CE-3 PCAM images at Point A (one of the rover sites where the rover is separated from the lander). This approach has produced accurate mosaics from CE-3 PCAM images, as is indicated by the value of the Peak Signal to Noise Ratio (PSNR), which is greater than 31 dB between the overlapped region of the images before and after fusion

Test Study on Airtight Capability of Filter Cakes for Slurry Shield and Its Application in a Case

To learn the airproof capacity of filter cakes as opening chambers under air pressure, a series of tests were carried out. The variations of discharged water with air pressure and time were observed, and the relationship between airproof capacity of filter cakes and surrounding air pressure was analysed. The test results indicated that there were three stages as compressed air acting on filter cakes: completely not infiltration, a very small amount of infiltration, and penetration leakage. The certain air pressure between the first and second stages was called the airproofing value of filter cake. And a capillary bundle model was used to explain the mechanism of air tightness of filter cakes. In Nanjing Yangtze River Tunnel, a 5 cm thickness filter cake was formed in gravel sand, and its airproofing value was a little lower than 0.12 MPa. The air pressure used as opening chamber should be equal to the summation of water pressure in sand and airproofing value of filter cake. While the air pressure is larger than the summation, the filter cake would be gas permeable. The slurry formulation and airproofing value of filter cakes obtained in the tests were applied successfully in Nanjing Yangtze River Tunnel

Fucoidan Does Not Exert Anti-Tumorigenic Effects on Uveal Melanoma Cell Lines

Background. The polysaccharide fucoidan is widely investigated as an anti-cancer agent. Here, we tested the effect of fucoidan on uveal melanoma cell lines. Methods. The effect of 100 µM fucoidan was investigated on five cell lines (92.1, Mel270 OMM1, OMM2.3, OMM2.5) and of 1 µg/mL–1 mg/mL fucoidan in two cell lines (OMM1, OMM2.3). Cell proliferation and viability were investigated with a WST-1 assay, migration in a wound healing (scratch) assay. Vascular Endothelial Growth Factor (VEGF) was measured in ELISA. Angiogenesis was evaluated in co-cultures with endothelial cells. Cell toxicity was induced by hydrogen-peroxide. Protein expression (Akt, ERK1/2, Bcl-2, Bax) was investigated in Western blot. Results. Fucoidan increased proliferation in two and reduced it in one cell line. Migration was reduced in three cell lines. The effect of fucoidan on VEGF was cell type and concentration dependent. In endothelial co-culture with 92.1, fucoidan significantly increased tubular structures. Moreover, fucoidan significantly protected all tested uveal melanoma cell lines from hydrogen-peroxide induced cell death. Under oxidative stress, fucoidan did not alter the expression of Bcl-2, Bax or ERK1/2, while inducing Akt expression in 92.1 cells but not in any other cell line. Conclusion. Fucoidan did not show anti-tumorigenic effects but displayed protective and pro-angiogenic properties, rendering fucoidan unsuitable as a potential new drug for the treatment of uveal melanoma

Influence of Fucoidan Extracts from Different Fucus Species on Adult Stem Cells and Molecular Mediators in In Vitro Models for Bone Formation and Vascularization

Fucoidans, sulfated polysaccharides extracted from brown algae, are marine products with the potential to modulate bone formation and vascularization processes. The bioactivity and safety of fucoidans are highly associated with their chemical structure, which may vary with algae species and extraction method. Thus, in depth evaluation of fucoidan extracts in terms of endotoxin content, cytotoxicity, and their detailed molecular biological impact on the individual cell types in bone is needed. In this study, we characterized fucoidan extracts from three different Fucus species including Fucus vesiculosus (Fv), Fucus serratus (Fs), and Fucus distichus subsp. evanescens (Fe) for their chemical features, endotoxin content, cytotoxicity, and bioactive effects on human outgrowth endothelial cells (OEC) and human mesenchymal stem cells (MSC) as in vitro models for bone function and vascularization. Extracts contained mainly high molecular weight (HMW) fucoidans and were free of endotoxins that may cause inflammation or influence vascularization. OEC tolerated fucoidan concentrations up to 200 µg/mL, and no indication of cytotoxicity was observed. The inflammatory response, however, investigated by real-time PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) and endothelial barrier assessed by impedance measurement differed for the individual extracts. MSC in comparison with endothelial cells were more sensitive to fucoidans and showed partly reduced metabolic activity and proliferation at higher doses of fucoidans. Further results for MSC indicated impaired osteogenic functions in alkaline phosphatase and calcification assays. All tested extracts consistently lowered important molecular mediators involved in angiogenesis, such a VEGF (vascular endothelial growth factor), ANG-1 (angiopoietin 1), and ANG-2 (angiopoietin 2), as indicated by RT-PCR and ELISA. This was associated with antiangiogenic effects at the functional level using selected extracts in co-culture models to mimic bone vascularization processes during bone regeneration or osteosarcoma

Facet-dependent growth of InAsP quantum wells in InP nanowire and nanomembrane arrays

Selective area epitaxy is a powerful growth technique that has been used to produce III-V semiconductor nanowire and nanomembrane arrays for photonic and electronic applications. The incorporation of a heterostructure such as quantum wells (QWs) brings new functionality and further broadens their applications. Using InP nanowires and nanomembranes as templates, we investigate the growth of InAsP QWs on these pure wurtzite nanostructures. InAsP QWs grow both axially and laterally on the nanowires and nanomembranes, forming a zinc blende phase axially and wurtzite phase on the sidewalls. On the non-polar {1100} sidewalls, the radial QW selectively grows on one sidewall which is located at the semi-polar 〈112〉 A side of the axial QW, causing the shape evolution of the nanowires from hexagonal to triangular cross section. For nanomembranes with {1100} sidewalls, the radial QW grows asymmetrically on the {1100} facet, destroying their symmetry. In comparison, nanomembranes with {1120} sidewalls are shown to be an ideal template for the growth of InAsP QWs, thanks to the uniform QW formation. These QWs emit strongly in the near IR region at room temperature and their emission can be tuned by changing their thickness or composition. These findings enrich our understanding of the QW growth, which provides new insights for heterostructure design in other III-V nanostructures.National Natural Science Foundation of China (No. 61974166, 51702368 and 61874141); Hunan Provincial Natural Science Foundation of China (2018JJ3684); Open Project of the State Key Laboratory of Luminescence and Applications (SKLA-2018-07); and The Australian Research Council (ARC) are acknowledged for financial support

2023 roadmap for potassium-ion batteries

The heavy reliance of lithium-ion batteries (LIBs) has caused rising concerns on the sustainability of lithium and transition metal and the ethic issue around mining practice. Developing alternative energy storage technologies beyond lithium has become a prominent slice of global energy research portfolio. The alternative technologies play a vital role in shaping the future landscape of energy storage, from electrified mobility to the efficient utilization of renewable energies and further to large-scale stationary energy storage. Potassium-ion batteries (PIBs) are a promising alternative given its chemical and economic benefits, making a strong competitor to LIBs and sodium-ion batteries for different applications. However, many are unknown regarding potassium storage processes in materials and how it differs from lithium and sodium and understanding of solid–liquid interfacial chemistry is massively insufficient in PIBs. Therefore, there remain outstanding issues to advance the commercial prospects of the PIB technology. This Roadmap highlights the up-to-date scientific and technological advances and the insights into solving challenging issues to accelerate the development of PIBs. We hope this Roadmap aids the wider PIB research community and provides a cross-referencing to other beyond lithium energy storage technologies in the fast-pacing research landscape

Vascularization and osteogenesis in bone physiological processes influenced by bioactive molecules and scaffolds

In bone tissue engineering, bioactive molecules which modulate bone growth and biocompatible biomaterials, which often provide biomimetic properties, are combined with progenitor or stem cells involved in bone physiological processes. This tissue replacement approach provides a new therapeutic strategy for bone repair and regeneration especially for larger bone defects which often occur after trauma or bone tumor resection for instance. In this context, the aim of this thesis is to investigate the detailed biological functions of new bioactive molecules. Further the thesis is focused on hydrogels used for instance as delivery systems of such bioactive factors or cells, aiming to develop the basis for new clinical applications in bone pre-vascularization and regeneration. First, this thesis assesses the impact of fucoidan extracts, a group of marine origin bioactive molecules, on the formation of vascular structures in co-culture models relevant for bone repair and in osteosarcoma. Human mesenchymal stem cells (MSCs) which can differentiate to the bone forming osteoblastic cells, respectively the osteosarcoma cell line MG63 and outgrowth endothelial cells (OECs) which possess a high potential of angiogenesis, were used to create co-culture models relevant for bone regeneration or osteosarcoma. Here the aim was to study the angiogenic and osteogenic regulatory effects of fucoidan in the co-cultures as functional units. In both types of co-culture models, crude fucoidan extracts significantly reduced angiogenesis which was associated with a decrease of angiogenic factors on the protein level, thus suggesting an impairing effect of this type of fucoidan on angiogenesis in co-cultures via binding and reducing the levels of free angiogenic factors VEGF and SDF-1 in the system. In the next part of this thesis, the physiological molecule vitamin D3 (calcitriol) was studied in terms of its potential to modulate the inflammatory or immunological response in a bone regeneration respectively infection co-culture model stimulated with LPS. Bone infections after the application of implant materials for instance are associated with severe clinical consequences. Vitamin D3 revealed a beneficial effect on angiogenic activity in co-cultures, as well as a positive effect on osteogenic activity. Here, especially the mineralization in the late osteogenic process was enhanced. However, although no significant impact on the inflammatory response was observed, Vitamin D3 induced an increase in the gene expression of the antimicrobial peptide LL-37 in MSC, thus indicating a potential beneficial influence on the immune defense in the bone in case of infection to be further refined. Last, the thesis examines bio-functionalized hydrogels for bone regeneration and tissue engineering. In this part, hydrogels consisting of mineralized silk fibroin fibers and UV-inducible gel-components induced excellent MSCs adherence along the bio-functionalized silk fiber component and a significant upregulation of VEGF expression level, thereby indicating a high potential of this injectable composite biomaterial for bone regeneration and vascularization. Overall, bioactive compounds and bio-functional scaffolds investigated in this thesis showed the ability to modulate angiogenesis on the cellular and molecular level, to trigger the immune defense or to serve as injectable bone materials guiding the performance of osteogenic cells via their biomimetic properties. Nevertheless, further research will be necessary to employ these approaches in a clinical setting for bone repair and regeneration

Cell-age and cell type-dependent behavior of human vascular cells on micro-structured or soft polymer substrates

Increasing number of studies are focused on how adherent cells respond, in vitro, to different properties of a material. Typical properties are the surface chemistry, topographical cues (at the nano- and micro-scale) of the surface, and the substrate stiffness. Cell Response studies are of importance for designing new biomaterials with applications in cell culture technologies, regenerative medicine, or for medical implants. However, only very few studies take the cell age factor, respectively the donor age, into account. In this work, we tested two types of human vascular cells (smooth muscle and endothelial cells) from old and young donors on (a) micro-structured surfaces made of pol (dimethylsiloxane) or on (b) flat polyacrylamide hydrogels with varying stiffnesses. These experiments reveal age-dependent and cell typedependent differences in the cell response to the topography and stiffness, and may establish the Basis for further studies focusing on cell age-dependent responses

Pathological Mechanistic Studies of Osimertinib Resistance in Non-Small-Cell Lung Cancer Cells Using an Integrative Metabolomics-Proteomics Analysis

Background. Osimertinib is the first-line therapeutic option for the T790M-mutant non-small-cell lung cancer and the acquired resistance obstructs its application. It is an urgent challenge to identify the potential mechanisms of osimertinib resistance for uncovering some novel therapeutic approaches. Methods. In the current study, the cell metabolomics based on ultra-high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry and the qualitative and tandem mass tags quantitative proteomics were performed. Results. 54 differential metabolites and 195 differentially expressed proteins were, respectively, identified. The amino acids metabolisms were significantly altered. HIF-1 signaling pathway modulating P-glycoproteins expression, PI3K-Akt pathway regulating survivin expression, and oxidative phosphorylation were upregulated, while arginine and proline metabolism regulating NO production and glycolysis/gluconeogenesis were downregulated during osimertinib resistance. Conclusion. The regulation of HIF-1 and PI3K-Akt signaling pathway, energy supply process, and amino acids metabolism are the promising therapeutic tactics for osimertinib resistance