Design on the Winter Jujubes Harvesting and Sorting Device

According to the existing problems of winter jujube harvesting, such as the intensive labor of manual picking, damage to the surface of winter jujubes, a winter jujube harvesting and sorting device was developed. This device consisted of vibration mechanism, collection mechanism, and sorting mechanism. The eccentric vibration mechanism made the winter jujubes fall, and the umbrella collecting mechanism can collect winter jujube and avoid the impact of winter jujube on the ground, and the sorting mechanism removed jujube leaves and divided the jujube into two types, and the automatic leveling mechanism made the device run smoothly in the field. Through finite element analysis and BP (Back Propagation) neural network analysis, the results show that: The vibration displacement of jujube tree is related to the trunk diameter and vibration position; the impact force of winter jujubes falling is related to the elastic modulus of umbrella material; the collecting area can be increased four times for each additional step of the collection mechanism; jujube leaves can be effectively removed when blower wind speed reaches 45.64 m/s. According to the evaluation standard grades of the jujubes harvesting and sorting, the device has good effects and the excellent rate up to 90%, which has good practicability and economy

Chemoselective Fluorogenic Bioconjugation of Vicinal Dithiol-Containing Proteins for Live Cellular Imaging via Small Molecular Conjugate Acceptors

To develop small molecular fluorogenic tools for the chemoselective labeling of vicinal dithiol-containing proteins (VDPs) in live cells is important for studying intracellular redox homeostasis. With this research, we developed small molecule-based fluorescent probes, achieving selective labeling of VDPs through thiol-thiol substitutions on bisvinylogous thioester conjugated acceptors (IDAs). Initially, IDAs demonstrated its ability to bridge vicinal cysteine-sulfhydryls on a peptide as a mimic. Then, the peptide complex could be decoupled to recover the original peptide-SH in the presence of dithiothreitol. Furthermore, fluorometric signal amplification of the fluorescent probes occurred with high sensitivity, low limit of detection, and selectivity toward vicinal dithiols on reduced bovine serum albumin, as an example of real world VDPs. More importantly, the probes were utilized successfully for labeling of endogenous VDPs at different redox states in live cells. Thus, the bisvinylogous thioester-based receptor as a functional probe represents a new platform for uncovering the function of VDPs in live cells.</p

Chemoselective Fluorogenic Bioconjugation of Vicinal Dithiol-Containing Proteins for Live Cellular Imaging via Small Molecular Conjugate Acceptors

To develop small molecular fluorogenic tools for the chemoselective labeling of vicinal dithiol-containing proteins (VDPs) in live cells is important for studying intracellular redox homeostasis. With this research, we developed small molecule-based fluorescent probes, achieving selective labeling of VDPs through thiol-thiol substitutions on bisvinylogous thioester conjugated acceptors (IDAs). Initially, IDAs demonstrated its ability to bridge vicinal cysteine-sulfhydryls on a peptide as a mimic. Then, the peptide complex could be decoupled to recover the original peptide-SH in the presence of dithiothreitol. Furthermore, fluorometric signal amplification of the fluorescent probes occurred with high sensitivity, low limit of detection, and selectivity toward vicinal dithiols on reduced bovine serum albumin, as an example of real world VDPs. More importantly, the probes were utilized successfully for labeling of endogenous VDPs at different redox states in live cells. Thus, the bisvinylogous thioester-based receptor as a functional probe represents a new platform for uncovering the function of VDPs in live cells.</p

Versatile Ratiometric Fluorescent Probe Based on the Two-Isophorone Fluorophore for Sensing Nitroxyl

Nitroxyl (HNO) is closely linked with numerous biological processes. Fluorescent probes provide a visual tool for determining HNO. Due to fluorescence quenching by HNO-responsive recognition groups, most of the current fluorescent probes exhibit an "off-on"fluorescence response. As such, the single fluorescence signal of these probes is easily affected by external factors such as the microenvironment, sensor concentration, and photobleaching. Herein, we have developed a ratiometric fluorescent probe (CHT-P) based on our previously developed two-isophorone fluorophore. CHT-P could be used to determine HNO through ratiometric signal readouts with high selectivity and sensitivity, ensuring the accurate quantitative detection of HNO. Additionally, the probe exhibited low cytotoxicity, was cell permeable, and could be used for ratiometric imaging of HNO in cells. Finally, CHT-P-coated portable test strips were used to determine HNO using the solid-state fluorescence signal readout. </p

Radar absorbing combinatorial metamaterial based on silicon carbide/carbon foam material embedded with split square ring metal

A broadband radar absorbing combinatorial foam metamaterial (CFMM) was developed and evaluated via simulation and experimentation. CFMM was constructed using silicon carbide/carbon (SiC/C) foam material, a FR4 dielectric material, and a metal pattern. The SiC/C foam material was prepared using a template. The metal pattern consists of a square ring with four split gaps in the middle of the ring. As a result of this new design, the influence of metal pattern design geometrical dimensions on the absorption performance of CFMM were discussed, as absorption performance can be adjusted by changing the geometric parameters of the metal patterns. The physical absorption mechanism was clarified through analysis of the field distribution and impedance. Finally, a lightweight broadband CFMM was designed. The designed CFMM exhibit a −10 dB absorption bandwidth from 4 GHz to 18 GHz with a total bulk density of 0.56 g/cm3. Additionally, the absorption performance of the designed CFMM in the case of oblique incidence was studied. Keywords: Foam materials, Metamaterial, Radar absorption performanc

Annual performance and dynamic characteristics of a hybrid wind-wave floating energy system at a localized site in the North Sea

Gao et al. (2021. Investigation on the effects of Bragg reflection on harbor oscillations. Coastal Engineering, 170: 103977) attempted to utilize Bragg reflection to alleviate harbor resonance, and discovered its significant effectiveness for the first time. The Bragg reflection occurred over periodic topographies deployed outside and parallel to the harbor entrance. However, the inherent mitigating mechanisms were not revealed. In this paper, the Bragg reflection over a patch of sinusoidal bars and its coupling effects with the harbor are studied using a Boussinesq-type model. Different from the previous study, the sinusoidal bars with various numbers and amplitudes are arranged in the manner of an arc layout around rather than parallel to the entrance. A physical process decomposition method is proposed to reveal the inherent mitigation mechanism. Moreover, the condition under which harbor resonance is not excited by incident waves (hereinafter referred to as "harbor non-resonance condition") is also investigated, and the similarities/differences of mitigation mechanisms between the harbor resonance and non-resonance conditions are discussed. Finally, for the harbor resonance condition, the effects of the number and the amplitude of sinusoidal bars on the best mitigation effect and optimal bar wavelength, which can attain the best mitigation effect, are systematically analyzed.Published version is available for viewing only. (See "Related URI")「関連URI」より出版社版の閲覧専用ページへリン

Dual-Function Fluorescent Probe for the Detection of Peroxynitrite and Adenosine Triphosphate

A novel dual-function fluorescent probe (ATP-LW) was developed for the detection of ONOO- and ATP. </p

Combining Magnetic Resonance Imaging with Photothermal Therapy of CuS@BSA Nanoparticles for Cancer Theranostics

The treatment of tumors has been a wide concern by a large number of scientific researchers. Combining magnetic resonance imaging (MRI) with photothermal therapy (PTT) enables cancer theranostics to be more efficient and accurate. Herein, we synthesized CuS@BSA nanoparticles with an average grain diameter of about 16.5 nm through a facile one-pot eco-friendly and user-friendly strategy and it was found to have strong near-infrared absorption property and magnetic resonance imaging ability so that it can be utilized as a multifunctional agent for cancer theranostic. The in vitro toxicity study showed that CuS@BSA nanoparticles with low toxicity could kill cancer cells with the 980 nm NIR laser effectively. Furthermore, they exhibit a certain value of relaxivity (<i>r</i>₁ = 0.26 mM^–1·s^–1) compared to that of clinically widely used reagent Magnevist (<i>r</i>₁ = 3.13 mM^–1·s^–1). The resulting product of CuS@BSA nanoparticles with their magnetic resonance imaging (MRI) and photothermal therapeutic capabilities could represent a kind of potential candidate for cancer theranostics

Dual-channel fluorescent probe for the simultaneous monitoring of peroxynitrite and adenosine-5’-triphosphate in cellular applications

The concentrations of ATP and ONOO− have been correlated with the progression a number of diseases including ischemia-reperfusion injury and drug-induced liver injury. Here, we report the development of fluorescent probe, ATP-LW, which enables the simultaneous detection of ONOO− and ATP. ONOO− selectively oxidises the boronate pinacol ester of ATP-LW, to afford the fluorescent 4-hydroxy-1,8-naphthalimide product NA-OH (λex = 450 nm, λem = 562 nm or λex = 488 nm, λem = 568 nm). While, the binding of ATP to ATP-LW induces the spirolactam ring opening of rhodamine to afford a highly emissive product (λex = 520 nm, λem = 587 nm). Due to the differences in emission between the ONOO− and ATP products, ATP-LW exhibits the unique ability to image ONOO− levels in the green channel (λex = 488 nm, λem = 500-575 nm) and ATP concentrations using the red channel (λex = 514 nm, λem = 575-650 nm). This was demonstrated using hepatocytes (HL-7702 cells) in cellular imaging experiments. The treatment of HL-7702 cell line with oligomycin A (an inhibitor of ATP synthase) resulted in a reduction of ATP in the red channel and increase in ONOO− green channel. While, the presence of SIN-1 (an exogenous ONOO− donor) results in an increase of ONOO−, and decrease in ATP. Significantly, when HL-7702 cells were treated with acetaminophen as a biological model for drug-induced liver injury, an increase in ONOO− green and decrease in ATP red channel fluorescence was observed. These results illustrate the utility of ATP-LW as a chemical tool to simultaneously monitor ATP and ONOO− concentrations in cellular-based applications