956 research outputs found
Derivation of generalized Young’s equation for cylindrical droplets between the outer surfaces of two tangent cylinders
The wetting properties of cylindrical droplets between the outer surfaces of two tangent cylinders are investigated by means of thermodynamics. For the three-phase system containing solid, liquid and vapor phases, a generalized Young equation for contact angles of cylindrical drops between the outer surfaces of two tangent cylinders has been thermodynamically derived. In fact, the theoretical foundation of the derived generalized Young’s equation is based on Gibbs’s capillary phenomena and the method of Rusanov’s dividing line
Research of the active reflector antenna using laser angle metrology system
Active reflector is one of the key technologies for constructing large
telescopes, especially for the millimeter/sub-millimeter radio telescopes. This
article introduces a new efficient laser angle metrology system for the active
reflector antenna of the large radio telescopes, with a plenty of active
reflector experiments mainly about the detecting precisions and the maintaining
of the surface shape in real time, on the 65-meter radio telescope prototype
constructed by Nanjing Institute of Astronomical Optics and Technology (NIAOT).
The test results indicate that the accuracy of the surface shape segmenting and
maintaining is up to micron dimension, and the time-response can be of the
order of minutes. Therefore, it is proved to be workable for the sub-millimeter
radio telescopes.Comment: 10 pages, 15 figure
Single-step bioconversion of lignocellulose to hydrogen using novel moderately thermophilic bacteria
BACKGROUND: Consolidated bioprocessing (CBP) of lignocellulosic biomass to hydrogen offers great potential for lower cost and higher efficiency compared to processes featuring dedicated cellulase production. Current studies on CBP-based hydrogen production mainly focus on using the thermophilic cellulolytic bacterium Clostridium thermocellum and the extremely thermophilic cellulolytic bacterium Caldicellulosiruptor saccharolyticus. However, no studies have demonstrated that the strains in the genus Thermoanaerobacterium could be used as the sole microorganism to accomplish both cellulose degradation and H(2) generation. RESULTS: We have specifically screened for moderately thermophilic cellulolytic bacteria enabling to produce hydrogen directly from conversion of lignocellulosic materials. Three new strains of thermophilic cellulolytic bacteria in the genus Thermoanaerobacterium growing at a temperature of 60°C were isolated. All of them grew well on various plant polymers including microcrystalline cellulose, filter paper, xylan, glucose, and xylose. In particular, the isolated bacterium, designated as Thermoanaerobacterium thermosaccharolyticum M18, showed high cellulolytic activity and a high yield of H(2). When it was grown in 0.5% microcrystalline cellulose, approximately 82% cellulose was consumed, and the H(2) yield and maximum production rate reached 10.86 mmol/g Avicel and 2.05 mmol/L/h, respectively. Natural lignocellulosic materials without any physicochemical or biological pretreatment also supported appreciable growth of strain M18, which resulted in 56.07% to 62.71% of insoluble cellulose and hemicellulose polymer degradation in corn cob, corn stalk, and wheat straw with a yield of 3.23 to 3.48 mmol H(2)/g substrate and an average production rate of 0.10 to 0.13 mmol H(2)/L/h. CONCLUSIONS: The newly isolated strain T. thermosaccharolyticum M18 displayed effective degradation of lignocellulose and produced large amounts of hydrogen. This is the first report of a Thermoanaerobacterium species presenting cellulolytic characteristics, and this species thus represents a novel cellulolytic bacterium distinguished from all other known cellulolytic bacteria. In comparison, the extraordinary yield and specific rate of hydrogen for strain M18 obtained from lignocellulose make it more attractive in monoculture fermentation. T. thermosaccharolyticum M18 is thus a potential candidate for rapid conversion of lignocellulose to biohydrogen in a single step
Comprehensive Identification and Expression Analyses of the SnRK Gene Family in Casuarina equisetifolia in Response to Salt Stress
Background Sucrose nonfermenting-1 (SNF1)-related protein kinases (SnRKs) play crucial roles in plant signaling pathways and stress adaptive responses by activating protein phosphorylation pathways. However, there have been no comprehensive studies of the SnRK gene family in the widely planted salt-tolerant tree species Casuarina equisetifolia. Here, we comprehensively analyze this gene family in C. equisetifolia using genome-wide identification, characterization, and profiling of expression changes in response to salt stress. Results A total of 26 CeqSnRK genes were identified, which were divided into three subfamilies (SnRK1, SnRK2, and SnRK3). The intron–exon structures and protein‑motif compositions were similar within each subgroup but differed among groups. Ka/Ks ratio analysis indicated that the CeqSnRK family has undergone purifying selection, and cis-regulatory element analysis suggested that these genes may be involved in plant development and responses to various environmental stresses. A heat map was generated using quantitative real‑time PCR (RT-qPCR) data from 26 CeqSnRK genes, suggesting that they were expressed in different tissues. We also examined the expression of all CeqSnRK genes under exposure to different salt concentrations using RT-qPCR, finding that most CeqSnRK genes were regulated by different salt treatments. Moreover, co-expression network analysis revealed synergistic effects among CeqSnRK genes. Conclusions Several CeqSnRK genes (CeqSnRK3.7, CeqSnRK3.16, CeqSnRK3.17) were up-regulated following salt treatment. Among them, CeqSnRK3.16 expression was significantly up-regulated under various salt treatments, identifying this as a candidate gene salt stress tolerance gene. In addition, CeqSnRK3.16 showed significant expression change correlations with multiple genes under salt stress, indicating that it might exhibit synergistic effects with other genes in response to salt stress. This comprehensive analysis will provide a theoretical reference for CeqSnRK gene functional verification and the role of these genes in salt tolerance
N-(2-Hydroxyethyl)-1,8-naphthalimide
In the molecule of the title compound, C14H11NO3, the naphthalimide ring system is nearly planar (r.m.s. deviation 0.0139 Å). In the crystal structure, intermolecular O—H⋯O hydrogen bonds link the molecules into centrosymmetric dimers forming R
2
2(14) ring motifs. π–π contacts between the naphthalimide rings [centroid–centroid distances = 3.648 (3), 3.783 (3), 3.635 (3), 3.722 (3) and 3.755 (3) Å] may further stabilize the structure
Determination of tetramethylpyrazine in rat plasma by liquid chromatography/electrospray mass spectrometry
A sensitive and selective liquid chromatography/electrospray mass spectrometry (LC-ESIMS)
method for determination of tetramethylpyrazine in rat plasma was developed. After addition of
phenacetin as internal standard, protein precipitation by acetonitrile was used as sample preparation.
Chromatographic separation was achieved on a Zorbax SB-C18 (2.1 mm×150 mm, 5 µm) column with
(40:60, v/v) acetonitrile-water containing 0.1 % formic acid as mobile phase. Electrospray ionization (ESI)
source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to
quantify tetramethylpyrazine using target fragment ions m/z 136.9 for tetramethylpyrazine and m/z 179.8
for the IS. Calibration plots were linear over the range of 20-4000 ng/mL for tetramethylpyrazine in plasma.
Lower limit of quantitation (LLOQ) for tetramethylpyrazine was 20 ng/mL. Mean recovery of tetramethylpyrazine
from plasma was in the range 95.4-97.2 %. RSD of intra-day and inter-day precision were
less than 9 %, respectively. This method is simple, sensitive and fast enough to be used in pharmacokinetic
research for determination of tetramethylpyrazine in rat plasma.Colegio de Farmacéuticos de la Provincia de Buenos Aire
Determination of tetramethylpyrazine in rat plasma by liquid chromatography/electrospray mass spectrometry
A sensitive and selective liquid chromatography/electrospray mass spectrometry (LC-ESIMS)
method for determination of tetramethylpyrazine in rat plasma was developed. After addition of
phenacetin as internal standard, protein precipitation by acetonitrile was used as sample preparation.
Chromatographic separation was achieved on a Zorbax SB-C18 (2.1 mm×150 mm, 5 µm) column with
(40:60, v/v) acetonitrile-water containing 0.1 % formic acid as mobile phase. Electrospray ionization (ESI)
source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to
quantify tetramethylpyrazine using target fragment ions m/z 136.9 for tetramethylpyrazine and m/z 179.8
for the IS. Calibration plots were linear over the range of 20-4000 ng/mL for tetramethylpyrazine in plasma.
Lower limit of quantitation (LLOQ) for tetramethylpyrazine was 20 ng/mL. Mean recovery of tetramethylpyrazine
from plasma was in the range 95.4-97.2 %. RSD of intra-day and inter-day precision were
less than 9 %, respectively. This method is simple, sensitive and fast enough to be used in pharmacokinetic
research for determination of tetramethylpyrazine in rat plasma.Colegio de Farmacéuticos de la Provincia de Buenos Aire
(R)-7-Bromo-2,3,4,4a-tetrahydro-1H-xanthen-1-one
The title compound, C13H11BrO2, contains a tricyclic ring system with one chiral center which exhibits an R configuration. The crystal structure is devoid of any classical hydrogen bonding
4-Bromo-2,6-dimethylanilinium bromide monohydrate
In the title compound, C8H11BrN+·Br−·H2O, a network of N—H⋯O, N—H⋯Br and O—H⋯Br hydrogen bonds helps to consolidate the crystal packing
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