62 research outputs found
Sca-1+ cardiac fibroblasts promote development of heart failure
The causative effect of GM-CSF produced by cardiac fibroblasts to development of heart failure has not been shown. We identified the pathological GM-CSF-producing cardiac fibroblast subset and the specific deletion of IL-17A signaling to these cells attenuated cardiac inflammation and heart failure. We describe here the CD45−CD31−CD29+mEFSK4+PDGFRα+Sca-1+periostin+ (Sca-1+) cardiac fibroblast subset as the main GM-CSF producer in both experimental autoimmune myocarditis and myocardial infarction mouse models. Specific ablation of IL-17A signaling to Sca-1+periostin+ cardiac fibroblasts (PostnCreIl17rafl/fl) protected mice from post-infarct heart failure and death. Moreover, PostnCreIl17rafl/fl mice had significantly fewer GM-CSF-producing Sca-1+ cardiac fibrob-lasts and inflammatory Ly6Chi monocytes in the heart. Sca-1+ cardiac fibroblasts were not only potent GM-CSF producers, but also exhibited plasticity and switched their cytokine production profiles depending on local microenvironments. Moreover, we also found GMCSF-positive cardiac fibroblasts in cardiac biopsy samples from heart failure patients of myocarditis or ischemic origin. Thus, this is the first identification of a pathological GMCSF-producing cardiac fibroblast subset in human and mice hearts with myocarditis and ischemic cardiomyopathy. Sca-1+ cardiac fibroblasts direct the type of immune cells infiltrating the heart during cardiac inflammation and drive the development of heart failure
NF-κB activity marks cells engaged in receptor editing
Because of the extreme diversity in immunoglobulin genes, tolerance mechanisms are necessary to ensure that B cells do not respond to self-antigens. One such tolerance mechanism is called receptor editing. If the B cell receptor (BCR) on an immature B cell recognizes self-antigen, it is down-regulated from the cell surface, and light chain gene rearrangement continues in an attempt to edit the autoreactive specificity. Analysis of a heterozygous mutant mouse in which the NF-κB–dependent IκBα gene was replaced with a lacZ (β-gal) reporter complementary DNA (cDNA; IκBα+/lacZ) suggests a potential role for NF-κB in receptor editing. Sorted β-gal+ pre–B cells showed increased levels of various markers of receptor editing. In IκBα+/lacZ reporter mice expressing either innocuous or self-specific knocked in BCRs, β-gal was preferentially expressed in pre–B cells from the mice with self-specific BCRs. Retroviral-mediated expression of a cDNA encoding an IκBα superrepressor in primary bone marrow cultures resulted in diminished germline κ and rearranged λ transcripts but similar levels of RAG expression as compared with controls. We found that IRF4 transcripts were up-regulated in β-gal+ pre–B cells. Because IRF4 is a target of NF-κB and is required for receptor editing, we suggest that NF-κB could be acting through IRF4 to regulate receptor editing
Bacterial Effector Binding to Ribosomal Protein S3 Subverts NF-κB Function
Enteric bacterial pathogens cause food borne disease, which constitutes an enormous economic and health burden. Enterohemorrhagic Escherichia coli (EHEC) causes a severe bloody diarrhea following transmission to humans through various means, including contaminated beef and vegetable products, water, or through contact with animals. EHEC also causes a potentially fatal kidney disease (hemolytic uremic syndrome) for which there is no effective treatment or prophylaxis. EHEC and other enteric pathogens (e.g., enteropathogenic E. coli (EPEC), Salmonella, Shigella, Yersinia) utilize a type III secretion system (T3SS) to inject virulence proteins (effectors) into host cells. While it is known that T3SS effectors subvert host cell function to promote diarrheal disease and bacterial transmission, in many cases, the mechanisms by which these effectors bind to host proteins and disrupt the normal function of intestinal epithelial cells have not been completely characterized. In this study, we present evidence that the E. coli O157:H7 nleH1 and nleH2 genes encode T3SS effectors that bind to the human ribosomal protein S3 (RPS3), a subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) transcriptional complexes. NleH1 and NleH2 co-localized with RPS3 in the cytoplasm, but not in cell nuclei. The N-terminal region of both NleH1 and NleH2 was required for binding to the N-terminus of RPS3. NleH1 and NleH2 are autophosphorylated Ser/Thr protein kinases, but their binding to RPS3 is independent of kinase activity. NleH1, but not NleH2, reduced the nuclear abundance of RPS3 without altering the p50 or p65 NF-κB subunits or affecting the phosphorylation state or abundance of the inhibitory NF-κB chaperone IκBα NleH1 repressed the transcription of a RPS3/NF-κB-dependent reporter plasmid, but did not inhibit the transcription of RPS3-independent reporters. In contrast, NleH2 stimulated RPS3-dependent transcription, as well as an AP-1-dependent reporter. We identified a region of NleH1 (N40-K45) that is at least partially responsible for the inhibitory activity of NleH1 toward RPS3. Deleting nleH1 from E. coli O157:H7 produced a hypervirulent phenotype in a gnotobiotic piglet model of Shiga toxin-producing E. coli infection. We suggest that NleH may disrupt host innate immune responses by binding to a cofactor of host transcriptional complexes
Specification of DNA Binding Activity of NF-κB Proteins
Nuclear factor-κB (NF-κB) is a pleiotropic mediator of inducible and specific gene regulation involving diverse biological activities including immune response, inflammation, cell proliferation, and death. The fine-tuning of the NF-κB DNA binding activity is essential for its fundamental function as a transcription factor. An increasing body of literature illustrates that this process can be elegantly and specifically controlled at multiple levels by different protein subsets. In particular, the recent identification of a non-Rel subunit of NF-κB itself provides a new way to understand the selective high-affinity DNA binding specificity of NF-κB conferred by a synergistic interaction within the whole complex. Here, we review the mechanism of the specification of DNA binding activity of NF-κB complexes, one of the most important aspects of NF-κB transcriptional control
The PARP1/ARTD1-Mediated Poly-ADP-Ribosylation and DNA Damage Repair in B Cell Diversification
ADP-ribosylation is an essential post-translational modification, mediated by a family of proteins named poly-ADP-ribose polymerases/Diphtheria toxin-like ADP-ribosyltransferases (PARPs/ARTDs), that functions to assist in cellular homeostasis through an array of mechanisms. Although the function of PARP1/ARTD1-mediated poly-ADP-ribosylation (PARylation) in response to environmental genotoxic stressors has been extensively studied, its role in the regulation and maintenance of cellular events under times of programmed DNA damage and repair remains to be elucidated. In the case of B cell maturation and differentiation, processes such as V(D)J recombination, somatic hypermutation, and class switch recombination, require the induction of DNA strand breaks for the generation of a varied immunoglobulin repertoire and, thus, serve as a model system to explore the function of PARylation in immunological processes. In this review, we summarize the current understanding of ADP-ribosylation and the PARPs/ARTDs family proteins, in particular PARP1/ARTD1-conferred PARylation, in B cells. Following an overview of PARylation in cellular responses to environmental and spontaneous DNA damage, we discuss the emerging function of PARP1/ARTD1 and PARylation in DNA damage-induced nuclear factor kappaB (NF-κB) signaling and B cell maturation and differentiation. Finally, we conclude by underlining further efforts that are needed to understand how the PARPs/ARTDs family proteins and ADP-ribosylation control the development and function of B cells
The PARP1/ARTD1-Mediated Poly-ADP-Ribosylation and DNA Damage Repair in B Cell Diversification
ADP-ribosylation is an essential post-translational modification, mediated by a family of proteins named poly-ADP-ribose polymerases/Diphtheria toxin-like ADP-ribosyltransferases (PARPs/ARTDs), that functions to assist in cellular homeostasis through an array of mechanisms. Although the function of PARP1/ARTD1-mediated poly-ADP-ribosylation (PARylation) in response to environmental genotoxic stressors has been extensively studied, its role in the regulation and maintenance of cellular events under times of programmed DNA damage and repair remains to be elucidated. In the case of B cell maturation and differentiation, processes such as V(D)J recombination, somatic hypermutation, and class switch recombination, require the induction of DNA strand breaks for the generation of a varied immunoglobulin repertoire and, thus, serve as a model system to explore the function of PARylation in immunological processes. In this review, we summarize the current understanding of ADP-ribosylation and the PARPs/ARTDs family proteins, in particular PARP1/ARTD1-conferred PARylation, in B cells. Following an overview of PARylation in cellular responses to environmental and spontaneous DNA damage, we discuss the emerging function of PARP1/ARTD1 and PARylation in DNA damage-induced nuclear factor kappaB (NF-κB) signaling and B cell maturation and differentiation. Finally, we conclude by underlining further efforts that are needed to understand how the PARPs/ARTDs family proteins and ADP-ribosylation control the development and function of B cells
Improved U-Net for Growth Stage Recognition of In-Field Maize
Precise recognition of maize growth stages in the field is one of the critical steps in conducting precision irrigation and crop growth evaluation. However, due to the ever-changing environmental factors and maize growth characteristics, traditional recognition methods usually suffer from limitations in recognizing different growth stages. For the purpose of tackling these issues, this study proposed an improved U-net by first using a cascade convolution-based network as the encoder with a strategy for backbone network replacement to optimize feature extraction and reuse. Secondly, three attention mechanism modules have been introduced to upgrade the decoder part of the original U-net, which highlighted critical regions and extracted more discriminative features of maize. Subsequently, a dilation path of the improved U-net was constructed by integrating dilated convolution layers using a multi-scale feature fusion approach to preserve the detailed spatial information of in-field maize. Finally, the improved U-net has been applied to recognize different growth stages of maize in the field. The results clearly demonstrated the superior ability of the improved U-net to precisely segment and recognize maize growth stage from in-field images. Specifically, the semantic segmentation network achieved a mean intersection over union (mIoU) of 94.51% and a mean pixel accuracy (mPA) of 96.93% in recognizing the maize growth stage with only 39.08 MB of parameters. In conclusion, the good trade-offs made in terms of accuracy and parameter number demonstrated that this study could lay a good foundation for implementing accurate maize growth stage recognition and long-term automatic growth monitoring
Protein Kinase A Phosphorylation Activates Vpr-Induced Cell Cycle Arrest during Human Immunodeficiency Virus Type 1 Infection▿
Infection with human immunodeficiency virus type 1 (HIV-1) causes an inexorable depletion of CD4+ T cells. The loss of these cells is particularly pronounced in the mucosal immune system during acute infection, and the data suggest that direct viral cytopathicity is a major factor. Cell cycle arrest caused by the HIV-1 accessory protein Vpr is strongly correlated with virus-induced cell death, and phosphorylation of Vpr serine 79 (S79) is required to activate G2/M cell cycle blockade. However, the kinase responsible for phosphorylating Vpr remains unknown. Our bioinformatic analyses revealed that S79 is part of a putative phosphorylation site recognized by protein kinase A (PKA). We show here that PKA interacts with Vpr and directly phosphorylates S79. Inhibition of PKA activity during HIV-1 infection abrogates Vpr cell cycle arrest. These findings provide new insight into the signaling event that activates Vpr cell cycle arrest, ultimately leading to the death of infected T cells
Crack Width Recognition of Tunnel Tube Sheet Based on YOLOv8 Algorithm and 3D Imaging
Based on the tunnel crack width identification, there are operating time constraints, limited operating space, high equipment testing costs, and other issues. In this paper, a large subway tunnel is a research object, and the tunnel rail inspection car is an operating platform equipped with industrial cameras in order to meet the requirements of the tunnel tube sheet crack width recognition of more than 0.2 mm, with the measuring instrument to verify that the tunnel rail inspection car in the state of uniform motion camera imaging quality has the reliability through the addition of laser rangefinders, the accurate measurement of the object distance and the calculation of the imaging plane and the angle of the plane to be measured, to amend the three-dimensional cracks. The pixel resolution of the image is corrected, the images imaged by the industrial camera are preprocessed, the YOLOv8 algorithm is used for the intelligent extraction of crack morphology, and finally, the actual width is calculated from the spacing between two points of the crack. The crack detection width obtained by image processing using the YOLOv8 algorithm is basically the same as the value of crack width obtained by manual detection, and the error rate of crack width detection ranges from 0% to 11%, with the average error rate remaining below 4%. Compared with the crack detection error rate of the Support Vector Machine (SVM), the crack extraction model is reduced by 1%, so using the tunnel inspection vehicle as a platform equipped with an industrial camera, YOLOv8 is used to realize the recognition of the shape and width of the cracks on the surface of the tunnel tube sheet to meet the requirements of a higher degree of accuracy. The number of pixels and the detection error rate are inversely proportional to each other. The angle between the imaging plane and the plane under test is directly proportional to the detection error rate. The angle between the vertical axis where the lens midpoint is located and the line connecting the shooting target and the lens center point is αi and the angle θi between the measured plane and the imaging plane is reciprocal, i.e., αi + θi = 90°. Therefore, using the inspection vehicle as a mobile platform equipped with an industrial camera and based on the YOLOv8 algorithm, the crack recognition of the tunnel tube sheet has the feasibility and the prospect of wide application, which provides a reference method for the detection of cracks in the tunnel tube sheet
Evaluation Method of Fatigue Life for Asphalt Pavement on the Steel Bridge Deck Based on the Inhomogeneous Poisson Stochastic Process
The paving layer on the steel box girder bridge deck is widely used when constructing pavements for steel bridges. Owing to the orthotropic feature of steel decks, a transverse clapboard and rib can lead to a concentration of stress. Consequently, fatigue cracks are often identified in asphalt concrete pavement layers due to re-compaction caused by heavy vehicles. This study aims to derive an evaluation method of fatigue life for asphalt pavement based on the inhomogeneous Poisson stochastic process in view of the highly random and uncertain working conditions of layered composite structures. According to the inhomogeneous Poisson stochastic process, along with Miner’s fatigue damage accumulation theory and the linear elastic fracture mechanics theory, the fatigue life formula could be deduced. Meanwhile, fatigue experiments for asphalt concrete are designed to investigate the correlation between the theoretical formula and the actual fatigue damage life of the material. Compared with the test, the accuracy error is within 10%, which is better than other traditional methods. Therefore, the fatigue life prediction model could better reflect the loading order effect and the interaction between loads, providing a new path for the fatigue reliability design of steel bridge deck asphalt pavement
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