Can coronary artery bypass surgery provide equivalent outcomes after percutaneous coronary interventions?

UNIFESP-EPMUNIFESP, EPMSciEL

O conceito do heart team nas doenças cardíacas: o paciente novamente como prioridade nas decisões médicas

Universidade Federal de São Paulo (UNIFESP) Escola Paulista de MedicinaFaculdade de Medicina de São José do Rio PretoUniversidade Estadual de CampinasUNIFESP, EPMSciEL

Evolución de la cirugía cardiovascular: la saga brasileña. Una historia de labor, vanguardia y éxito

Faculdade Estadual de Medicina de S.J. Rio PretoUnicamp Faculdade de Ciências MédicasUniversidade Federal de São Paulo (UNIFESP), Escola Paulista de Medicina (EPM)UNIFESP, EPMSciEL

The troubled heparin issue in the Brazilian market and the search for solutions

Universidade Federal de São Paulo (UNIFESP) Escola Paulista de MedicinaSão Paulo Medical JournalFAMERPUNICAMPUNIFESP, EPMSciEL

On-pump versus off-pump coronary artery bypass surgery: the impact on costs of health care systems

Universidade Federal de São Paulo (UNIFESP) Escola Paulista de MedicinaFaculdade de Medicina de São José do Rio PretoUniversidade de CampinasUNIFESP, EPMSciEL

SYNTAX trial: analysis and clinical implications

Universidade Federal de São Paulo (UNIFESP) Escola Paulista de MedicinaFAMERPUNICAMPUNIFESP, EPMSciEL

Quality of valve prostheses. Are we treating our patients well in the Brazilian National Health System?

Univ Fed Sao Paulo, Escola Paulista Med, Disciplina Cirurgia Cardiovasc, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Disciplina Cirurgia Cardiovasc, Sao Paulo, BrazilWeb of Scienc

The future of saphenous vein graft for coronary artery

Hospital da Universidade de OrebroEscola Paulista de MedicinaUNIFESP, EPMSciEL

Identification, Selection, and Enrichment of Cardiomyocyte Precursors

The large-scale production of cardiomyocytes is a key step in the development of cell therapy and tissue engineering to treat cardiovascular diseases, particularly those caused by ischemia. the main objective of this study was to establish a procedure for the efficient production of cardiomyocytes by reprogramming mesenchymal stem cells from adipose tissue. First, lentiviral vectors expressing neoR and GFP under the control of promoters expressed specifically during cardiomyogenesis were constructed to monitor cell reprogramming into precardiomyocytes and to select cells for amplification and characterization. Cellular reprogramming was performed using 5'-azacytidine followed by electroporation with plasmid pOKS2a, which expressed Oct4, Sox2, and Klf4. Under these conditions, GFP expression began only after transfection with pOKS2a, and less than 0.015% of cells were GFP(+). These GFP(+) cells were selected for G418 resistance to find molecular markers of cardiomyocytes by RT-PCR and immunocytochemistry. Both genetic and protein markers of cardiomyocytes were present in the selected cells, with some variations among them. Cell doubling time did not change after selection. Together, these results indicate that enrichment with vectors expressing GFP and neoR under cardiomyocyte-specific promoters can produce large numbers of cardiomyocyte precursors (CMPs), which can then be differentiated terminally for cell therapy and tissue engineering.Universidade Federal de São Paulo, Gene Therapy Invest Ctr, Dept Biophys, BR-04044010 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Surg, BR-04044010 São Paulo, BrazilUniversidade Federal de São Paulo, Gene Therapy Invest Ctr, Dept Biophys, BR-04044010 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Surg, BR-04044010 São Paulo, BrazilWeb of Scienc

The rescue surgeon

Universidade Federal de São Paulo (UNIFESP) Escola Paulista de MedicinaUniversidade Estadual do Oeste do ParanáFaculdade de Medicina de São José do Rio PretoUnicampUNIFESP, EPMSciEL