Water and wastewater strategic township plan Warragul, Victoria

In the past, water authorities have been undertaking capital works expenditure on a forward planning window of 18 months. Now under a more regulated State Government regime, water authorities have to prepare more detailed plans showing future capital expenditure over a five year period. These plans are then used to set price tariffs for the business to cover those costs over that period. With this in mind, the main objective of this dissertation was to prepare water and wastewater strategic plans for Warragul, Victoria. These plans would then be adopted by the organisation. Any recommendations from these strategic plans are then forwarded onto Gippsland Water’s asset improvement department to undergo more detailed planning and costing. These plans and costs are then submitted to the regulatory for approval in Gippsland Water’s submission of the water plan. Water Plan No.2 is due for submission by March 2007

Space Station Operations Capabilities in a Shoebox: Marshall Space Flight Center’s Telescience Resource Kit

The International Space Station (ISS) has provided the world an unprecedented capability, establishing a continuous human foothold in outer space for more than 22 years now. But maintaining that capability and supporting the ambitious portfolio of scientific research it hosts has required another unprecedented capability – providing ground-based operations support for the crew and a diverse manifest of research payloads, all simultaneously. To help meet this challenge, NASA’s Marshall Space Flight Center (MSFC) developed TReK, the Telescience Resource Kit, providing a robust solution for data, command, metadata, and file transfer capabilities. In response to an increasingly wide and diverse need for operations support resources, the TReK team has worked to find ways to make the software more flexible in order to support a wide range of missions. Today it has supported not only hundreds of ISS payloads, but also free-flying spacecraft and even aircraft-based research. This presentation will discuss how the team has modified capabilities needed to enable 24/7/365 research aboard ISS to provide the flexibility needed to support Small Sat missions, going back to one of MSFC’s first “minisatellite” missions in 2010 and beyond

Using Elo Rating as a Metric for Comparative Judgement in Educational Assessment

Marking and feedback are essential features of teaching and learning, across the overwhelming majority of educational settings and contexts. However, it can take a great deal of time and effort for teachers to mark assessments, and to provide useful feedback to the students. Furthermore, it also creates a significant cognitive load on the assessors, especially in ensuring fairness and equity. Therefore, an alternative approach to marking called comparative judgement (CJ) has been proposed in the educational space. Inspired by the law of comparative judgment (LCJ). This pairwise comparison for as many pairs as possible can then be used to rank all submissions. Studies suggest that CJ is highly reliable and accurate while making it quick for the teachers. Alternative studies have questioned this claim suggesting that the process can increase bias in the results as the same submission is shown many times to an assessor for increasing reliability. Additionally, studies have also found that CJ can result in the overall marking process taking longer than a more traditional method of marking as information about many pairs must be collected. In this paper, we investigate Elo, which has been extensively used in rating players in zero-sum games such as chess. We experimented on a large-scale Twitter dataset on the topic of a recent major UK political event ("Brexit", the UK's political exit from the European Union) to ask users which tweet they found funnier between a pair selected from ten tweets. Our analysis of the data reveals that the Elo rating is statistically significantly similar to the CJ ranking with a Kendall's tau score of 0.96 and a p-value of 1.5x10^(-5). We finish with an informed discussion regarding the potential wider application of this approach to a range of educational contexts.Comment: 12 pages, 4 figures, one table, pre-review versio

Mission Operations, Cubed: NASA Marshall Operations Support for SmallSats

SmallSats have come a long way since the Huntsville Operations Support Center (HOSC) at NASA’s Marshall Space Flight Center supported its first “minisatellite” mission in 2010. And just as SmallSats themselves have evolved in those 12 years, so too has the HOSC’s mission support for SmallSats. Marshall Space Flight Center has a long history with payload and mission operations, including support for the Apollo missions to the moon, the Space Shuttle program, and 21 years of continuous around-the-clock science operations support for research aboard the International Space Station. Today, the HOSC is a multi-tenant facility, supporting not only ISS, but also NASA’s Commercial Crew program, the Space Launch System, the Hubble and Chandra observatories and others – including multiple SmallSat missions. Two SmallSat solar sail missions will be among those taking advantage of the HOSC’s resources for planning, training for and executing mission operations – the Near Earth Asteroid (NEA) Scout and Solar Cruiser missions. One of 10 6U CubeSats manifest on the Artemis I launch of NASA’s Space Launch System rocket this year, NEA Scout’s three-year mission will be supported through a more traditional operations concept, with a dedicated Flight Controller staff operating within the HOSC. Scheduled to launch as part of the Interstellar Mapping and Acceleration Probe (IMAP) in February 2025, Solar Cruiser’s 11-month mission will take a next[1]generation approach to operations by utilizing a multi-mission flight controller concept, as well as Marshall’s Telescience Resource Kit (TreK). TreK provides a suite of software applications and libraries that allow the Mission Operations Center to serve as an in-house ground system which incorporates remote and automation capability options for engineers and scientists. This presentation will compare the approaches the HOSC will use to support these two missions as a way of demonstrating the array of options NASA MSFC offers for operations support for CubeSat and SmallSat missions

Cortical Inactivation by Cooling in Small Animals

Reversible inactivation of the cortex by surface cooling is a powerful method for studying the function of a particular area. Implanted cooling cryoloops have been used to study the role of individual cortical areas in auditory processing of awake-behaving cats. Cryoloops have also been used in rodents for reversible inactivation of the cortex, but recently there has been a concern that the cryoloop may also cool non-cortical structures either directly or via the perfusion of blood, cooled as it passed close to the cooling loop. In this study we have confirmed that the loop can inactivate most of the auditory cortex without causing a significant reduction in temperature of the auditory thalamus or other subcortical structures. We placed a cryoloop on the surface of the guinea pig cortex, cooled it to 2°C and measured thermal gradients across the neocortical surface. We found that the temperature dropped to 20–24°C among cells within a radius of about 2.5 mm away from the loop. This temperature drop was sufficient to reduce activity of most cortical cells and led to the inactivation of almost the entire auditory region. When the temperature of thalamus, midbrain, and middle ear were measured directly during cortical cooling, there was a small drop in temperature (about 4°C) but this was not sufficient to directly reduce neural activity. In an effort to visualize the extent of neural inactivation we measured the uptake of thallium ions following an intravenous injection. This confirmed that there was a large reduction of activity across much of the ipsilateral cortex and only a small reduction in subcortical structures

Cyclic AMP activates B-Raf and ERK in cyst epithelial cells from autosomal-dominant polycystic kidneys

Cyclic AMP activates B-Raf and ERK in cyst epithelial cells from autosomal-dominant polycystic kidneys.BackgroundThe proliferation of mural epithelial cells is a major cause of progressive cyst enlargement in autosomal-dominant polycystic kidney disease (ADPKD). Adenosine 3′, 5′ cyclic monophosphate (cAMP) stimulates the proliferation of cells from ADPKD cysts, but not cells from normal human kidney cortex (HKC), through the activation of protein kinase A (PKA), mitogen-activated protein kinase kinase (MEK), and extracellular signal-regulated kinase (ERK/MAPK). In the current study, we examined the signaling pathway between PKA and MEK in ADPKD and HKC cells.MethodsPrimary cultures of human ADPKD and HKC cells were prepared from nephrectomy specimens. We determined the effects of cAMP and epidermal growth factor (EGF) on the activation of ERK, B-Raf and Raf-1 in ADPKD and HKC cells by immune kinase assay and Western blot.Results8-Br-cAMP increased phosphorylated ERK (2.7- ± 0.6-fold, N = 7), and B-Raf kinase activity (3.6- ± 1.1-fold, N = 5) in cells from ADPKD kidneys; levels of phosphorylated Raf-1 were not changed. Inhibition of PKA by H89 strikingly decreased cAMP-stimulated phosphorylation of ERK and B-Raf, and MAPK inhibition by PD98059 blocked the effect of the nucleotide to activate ERK. By contrast, in HKC cells 8-Br-cAMP did not activate B-Raf and ERK. EGF stimulated the phosphorylation of ERK and Raf-1 in both ADPKD and HKC cells, but had no effect on B-Raf. 8-Br-cAMP and EGF conjointly increased ERK activation above that of either agonist alone in ADPKD cells, and this combined effect was abolished by PD98059, indicating that ERK was activated by EGF- and cAMP-responsive cascades that converge at MAPK.ConclusioncAMP activates ERK and increases proliferation of ADPKD epithelial cells, but not cells from normal human kidney cortex, through the sequential phosphorylation of PKA, B-Raf and MAPK in a pathway separate from, but complementary to, the classical receptor tyrosine kinase cascade. Consequently, cAMP and EGF have great potential to accelerate the progressive enlargement of renal cysts

The NASA Solar Cruiser Mission - Solar Sail Propulsion Enabling Heliophysics Missions

Solar Cruiser is a Small Satellite Technology Demonstration Mission (TDM) to mature solar sail propulsion technology to enable near-term, high-priority breakthrough science missions as defined in the Solar and Space Physics Decadal Survey. Solar sails have the potential to provide high ΔV for many types of missions. Solar sails are large, mirror-like structures made of a lightweight material that reflects sunlight to propel the spacecraft. The continuous solar photon pressure provides thrust with no need for the heavy, expendable propellants used by conventional chemical and electric propulsion systems. Solar Cruiser will demonstrate a “sailcraft” platform with pointing control and attitude stability comparable to traditional platforms, upon which a new class of Heliophysics missions may fly. It will show sailcraft operation (acceleration, navigation, station keeping, heliocentric plane change) scalability of sail technologies such as the boom, membrane, and deployer to enable more demanding missions, such as high inclination solar imaging. Solar Cruiser will launch as a secondary payload with NASA’s Interstellar Mapping and Acceleration Probe (IMAP) in early 2025. The sailcraft will separate from the launch vehicle on a near-L1 trajectory (Sun-Earth Lagrangian Point 1; sunward of L1 along the Sun-Earth Line) and complete its primary mission in 11 months or less

Near Earth Asteroid Scout - Mission Update

After its deployment from NASA’s Space Launch System (SLS), the Near-Earth Asteroid (NEA) Scout mission will travel to and image an asteroid during a close flyby using an 86m2 solar sail as its primary propulsion. Solar sails are large, mirror-like structures made of a lightweight material that reflects sunlight to propel the spacecraft. The continuous solar photon pressure provides thrust with no need for the heavy, expendable propellants used by conventional chemical and electric propulsion systems. Developed by NASA’s Marshall Space Flight Center (MSFC) and Jet Propulsion Laboratory (JPL), the NEA Scout is based on the industry-standard CubeSat form factor. The spacecraft measures 11 cm x 24 cm x 36 cm and weighs less than 14 kilograms. Following deployment from the Space Launch System (SLS), the solar sail will deploy, and the spacecraft will begin its 2.0 – 2.5-year journey. About one month before the asteroid flyby, NEA Scout will search for the target and start its Approach Phase using a combination of radio tracking and optical navigation and perform a relatively slow flyby (10-20 m/s) of the target. A summary of the mission, sailcraft, mission design, and its first several months of deep space operation will be described

Global Gene Expression Profiling in PPAR-γ Agonist-Treated Kidneys in an Orthologous Rat Model of Human Autosomal Recessive Polycystic Kidney Disease

Kidneys are enlarged by aberrant proliferation of tubule epithelial cells leading to the formation of numerous cysts, nephron loss, and interstitial fibrosis in polycystic kidney disease (PKD). Pioglitazone (PIO), a PPAR-γ agonist, decreased cell proliferation, interstitial fibrosis, and inflammation, and ameliorated PKD progression in PCK rats (Am. J. Physiol.-Renal, 2011). To explore genetic mechanisms involved, changes in global gene expression were analyzed. By Gene Set Enrichment Analysis of 30655 genes, 13 of the top 20 downregulated gene ontology biological process gene sets and six of the top 20 curated gene set canonical pathways identified to be downregulated by PIOtreatment were related to cell cycle and proliferation, including EGF, PDGF and JNK pathways. Their relevant pathways were identified using the Kyoto Encyclopedia of Gene and Genomes database. Stearoyl-coenzyme A desaturase 1 is a key enzyme in fatty acid metabolism found in the top 5 genes downregulated by PIO treatment. Immunohistochemical analysis revealed that the gene product of this enzyme was highly expressed in PCK kidneys and decreased by PIO. These data show that PIO alters the expression of genes involved in cell cycle progression, cell proliferation, and fatty acid metabolism

The Influence of Literacy on Patient-Reported Experiences of Diabetes Self-Management Support

Variability in disease-related outcomes may relate to how patients experience self-management support in clinical settings