Reduction of radiation dose using 80 kV tube voltage: a feasible strategy?

Cardiolog

Iliolumbar membrane, a newly recognised structure in the back

Despite intensive research in the anatomical sciences for the last two centuries, some structures of the human body still remain controversial or incompletely described. We describe a new membranous fascial anatomical entity, which we refer to as the iliolumbar membrane (ILM). During the 2004-2005 academic semesters at the American University of the Caribbean School of Medicine we dissected 40 human cadavers fixed in formalin-alcohol-phenol solution. Iliolumbar membrane is a thick connective tissue structure, deep to the skin, originating from the fibres of the thoracolumbar fascia at the lateral border of the erector spinae. It runs inferior to the superior border of the iliac crest, lateral to the posterior superior iliac spine, overlying the iliac crest at the level of the 4th lumbar vertebra. Iliolumbar membrane terminates within subcutaneous fat, where it divides into multiple layers. All cadavers showed considerable variation in the blending of the membrane’s multiple layers with the subcutaneous fat. However, all specimens consistently showed a uniform appearance of ILM at the point of origin. Iliolumbar membrane could be demonstrated objectively by ultrasound examination with a frequency of 7.5 MHz and also with a Stryker endoscope. A hypothesis is put forth, conjecturing that this new structure may have relevance in creating a natural barrier between the musculature of the back and the muscles of the gluteal region, similar to Scarpa’s fascia of the anterior abdominal wall

Increased accuracy in computed tomography coronary angiography; a new body surface area adapted protocol

Vascular Biology and Interventio

IVUS detects more coronary calcifications than MSCT; matter of both resolution and cross-sectional assessment?

Vascular Biology and Interventio

Positron emission tomography; viable tool in patients pre-CABG?

Vascular Biology and Interventio

The changing microRNA landscape by color and cloudiness:a cautionary tale for nipple aspirate fluid biomarker analysis

Purpose: Investigation of nipple aspirate fluid (NAF)-based microRNAs (miRNAs) as a potential screening tool for women at increased risk of developing breast cancer is the scope of our research. While aiming to identify discriminating NAF-miRNAs between women with different mammographic densities, we were confronted with an unexpected confounder: NAF sample appearance. Here we report and alert for the impact of NAF color and cloudiness on miRNA assessment. Methods: Seven classes of NAF colors coupled with cloudiness appearance were established. Using 173 NAF samples from 154 healthy women (19 samples were bilaterally collected), the expression of 14 target and 2 candidate endogenous control (EC) miRNAs was investigated using Taqman Advanced miRNA assays to identify significant differential expression patterns between color-cloudiness classes. Inter- and intra-individual variation of miRNA expression was analyzed using the coefficient of variation (CV). Results: We found that between the seven NAF classes, fold change miRNA expression differences ranged between 2.4 and 19.6 depending on the interrogated miRNA. Clear NAF samples exhibited higher miRNA expression levels compared to cloudy NAF samples with fold change differences ranging between 1.1 and 6.2. Inter-individual and intra-individual miRNA expression was fairly stable (CV &lt; 15 %), but nevertheless impacted by NAF sample appearance. Within NAF classes, inter-individual variation was largest for green samples (CV 6-15 %) and smallest for bloody samples (CV 2-6 %). Conclusions: Our data indicate that NAF color and cloudiness influence miRNA expression and should, therefore, be systematically registered using an objective color classification system. Given that sample appearance is an inherent feature of NAF, these variables should be statistically controlled for in multivariate data analyses. This cautionary note and recommendations could be of value beyond the field of NAF-miRNAs, given that variability in sample color and cloudiness is likewise observed in liquid biopsies such as urine, cerebrospinal fluid and sputum, and could thereby influence the levels of miRNAs and other biomarkers.</p