The HPLC/DAD Fingerprints and Chemometric Analysis of Flavonoid Extracts from the Selected Sage (Salvia) Species

The results of spectrophotometric and HPLC/DAD analysis are discussed, and a comparison is made of selectively extracted flavonoid fractions derived from twenty six sage species belonging to the Salvia genus. The sage samples were harvested in the vegetation seasons 2007, 2008, and 2009. It was a goal of this study to find out which species contain the highest yields of flavonoids (recognized for their free-radical-scavenging activity), as those with the highest yields could be applied in official medicine. It was spectrophotometrically established that the four sage species can be recognized for their highest flavonoid levels, while the HPLC/DAD analysis pointed out to the four other species. The source of the discrepancy between the two evaluation approaches was discussed.Moreover, the HPLC/DAD fingerprints of the flavonoid fraction underwent a chemometric pre-treatment, and then the purified fingerprints were analyzed by means of Principal Component Analysis (PCA) for the differences in the harvesting period. A difference was revealed between the herbs harvested in the 2007 season, and those harvested in 2008 and 2009. The main source of this difference could be the seasonal weather variation and the relatively longest storage period with the plants harvested in 2007

The 42nd Symposium Chromatographic Methods of Investigating Organic Compounds : Book of abstracts

The 42nd Symposium Chromatographic Methods of Investigating Organic Compounds : Book of abstracts. June 4-7, 2019, Szczyrk, Polan

Liquid chromatography fingerprint analysis and antioxidant activity of selected lavender species with chemometric calculations.

The extracts of seven Lavandulae species (Lavandula stoechas, Lavandula lanata, Lavandula viridis, Lavandula angustifolia "Rosea", Lavandula angustifolia "Afropurpurea", Lavandula angustifolia and one unknown) were analyzed using the reversed-phase-high performance liquid chromatography-diode array detection (RP-HPLC-DAD) with gradient elution technique to obtain the chromatographic fingerprint profiles. The HPLC analysis was performed using the Kinetex RP18 chromatographic column and eluent consisting of methanol-water-0.1% formic acid (5-100% (v/v)) at 30 °C with the run time of 60 min. and the detection wavelength 280 nm. The chromatograms were preliminary processed with the smoothing, noise reduction, background subtraction and alignment using the SpecAlign program (version 2.4.1). The presence of selected standards (apigenin, myricetin, luteolin, luteolin 7-glucoside, chlorogenic acid, caffeic acid, ferulic acid) in the extracts was confirmed. The chemical similarity between studied plants was evaluated using the Cluster Analysis (Pearson correlation coefficient, r, and Euclidean) and PCA. The preliminary antioxidant activity of studied extracts was evaluated based on the total phenolic content (Folin-Ciocalteu method), ferric ion reducing antioxidant parameter (FRAP) and α,α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method using the spectrophotometric technique

Thin layer chromatography in drug analysis

xxvii, 1039 p.; 26 c

Selected polyphenol encapsulation strategies to improve their stability

Polyphenols are one of the most numerous and ubiquitous groups of secondary plant metabolites, and constitute an integral part of both human and animal diets. These compounds possess a high spectrum of biological activities, including antioxidant, antibacterial, antiviral, anti-inflammatory, neuroprotective and cardioprotective. A lot of preclinical research and epidemiological data suggests that plant polyphenols reduce the risks of neurodegenerative diseases, cardiovascular disease, osteoporosis or diabetes and can slow the progression of cancers. These facts sugest that plant polyphenols might act as potential chemopreventive and anti-cancer agents. However, the levels of polyphenols that appear effective in vitro are often of an order of magnitude higher than the concentrations determined in vivo. This is a serious problem, as only a small part of the substance remain available following oral administration, due to insufficient gastric residence time, low permeability and solubility within the gut. An important element is polyphenols instability under conditions encountered in food processing and storage (oxygen, temperature, light), or in the gastrointestinal tract (enzymes, pH, other nutrients), all of which limit the activity of polyphenolic compounds. Another unfortunate trait of polypheonls is their potential unpleasant taste. In order to overcome these drawbacks, various formulation methods have been developed. Among them, encapsulation seems to be a promising technique to improve the effectiveness and the bioactivity of polyphenols. Moreover, it protects the core material from environmental factors. Microcapsules are small particulates that may range from submicron to several millimeters in size. Encapsulation methods can be classified in three groups: physical, physico-chemical, and chemical. The research studies reported in this paper revealed useful strategies to provide remarkable protection against harmful factors of polyphenolic compounds, avoiding the loss in activity and improving their bioavailability

TLC-MS Versus TLC-LC-MS Fingerprints of Herbal Extracts. Part III. Application of the Reversed-Phase Liquid Chromatography Systems With C18 Stationary Phase

Free full text: [https://doi.org/10.1093/chrsci/49.7.560

TLC-MS Versus TLC-LC-MS Fingerprints of Herbal Extracts. Part III. Application of the Reversed-Phase liquid Chromatography Systems With C-18 Stationary Phase

Free full text: [https://doi.org/10.1093/chrsci/49.7.560