150 research outputs found

    RhoH Regulates Subcellular Localization of ZAP-70 and Lck in T Cell Receptor Signaling

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    RhoH is an hematopoietic-specific, GTPase-deficient Rho GTPase that plays a role in T development. We investigated the mechanisms of RhoH function in TCR signaling. We found that the association between Lck and CD3ζ was impaired in RhoH-deficient T cells, due to defective translocation of both Lck and ZAP-70 to the immunological synapse. RhoH with Lck and ZAP-70 localizes in the detergent-soluble membrane fraction where the complex is associated with CD3ζ phosphorylation. To determine if impaired translocation of ZAP-70 was a major determinant of defective T cell development, Rhoh-/- bone marrow cells were transduced with a chimeric myristoylation-tagged ZAP-70. Myr-ZAP-70 transduced cells partially reversed the in vivo defects of RhoH-associated thymic development and TCR signaling. Together, our results suggest that RhoH regulates TCR signaling via recruitment of ZAP-70 and Lck to CD3ζ in the immunological synapse. Thus, we define a new function for a RhoH GTPase as an adaptor molecule in TCR signaling pathway

    Stanford type B aortic dissections. Current situation in 2016 and retrospection of 1990

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    The treatment of type B dissection has evolved in the last few decades due to continuous development, including new interventional techniques that have been implemented into clinical treatment. Today, patients with this disease entity present less often to cardiac surgeons due to various factors, particularly due to sufficient treatment options using new interventional techniques with less trauma by vascular surgeons. The aim of this article is to present our assessment from 1990 and compare it with current knowledge and experience in the treatment of type B dissection, and to critically analyze the current situation
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