Different Approaches For Protein Engineering In Industrial Biotechnology

Protein engineering is the novel field which has wide applications from pharmaceutics, industry, commercial, laundry and research. It may apply rational design or non rational design or both. Site directed mutagenesis is a classical approach involving the protein folding principles and as such different techniques involving multidisciplinary research and broad knowledge is required involving biocomputing of complex data obtained from various sequencing projects and prediction of the future protein structure either chemically or genetically modified. Non rational mutagenesis or directed evolution involves random mutations in the gene encoding protein or shuffling the genes encoding different domains producing a random set of numerous large libraries of mutant proteins, using advanced technology the desired protein can be selected but the exact structure or changes may remain unnoticed

Molecular Bio-imprinting of Biocatalysts

Energy conservation is the cry of the day. Attempts are made all over the world to occupy and use energy reserves. Increased industrialization and mechanization has led to the depletion of natural energy reserves. Its unavoidable to search for renewable sources of energy, which may be not used now but can be used by future generations. We are using the expertise of our ancestors. Thus exploiting the nature and newer techniques in this area would yield the best results. Bio-imprinting is one of those techniques whereby chemical modification is done in order to achieve highly expressed protein which can be stored in its highly active form in the specific solvent

Factor that effect on consumer behavior: The case of boarding students

The objective of this paper is to investigate the buying behavior of boarding students in Pakistan. This is basically an empirical study and a scale was developed to find out the impact of nine variables namely Basic needs, Former experience, friend and family, Recommended by celebrities, product design , Product quality, Product price , Brand and Discount on price. The questionnaire was distributed among the respondents on the basis of convenience sampling.The results showed that the buying behavior of boarding students are affected all of these factors which are proposed by literature and theoretical framework. Findings from this study provide a useful framework for marketers to link their services; Basic needs, Former experience, friend and family, Recommended by celebrities, product design ,Product quality, Product price , Brand and Discount on price thus enhancing their productivity and profitability in the Pakistani Telenor sector.In order to increase the external validity of results future research can be done using longitudinal designs

Stroke rehabilitation in pakistan: what we know and what we need to do?

Stroke is one of the leading causes of adult neurological disability all around the globe. There have been some remarkable improvements in the acute management of stroke. These include the use of thrombolysis, development of radiological investigations including computerized tomographic scan and magnetic resonance imaging and establishment of acute stroke units. There have been some promising advances the field of stroke rehabilitation as well in the last three decades

Abstracts from the 7th annual neurology research day, shifa international hospital, islamabad

:It\u27s a cross sectional study conducted at CP clinic of Armed Forces institute of Rehabilitation medicine Rawalpindi from Jan 2012 to Jan 2013. All cerebral palsy children above 2 years of age and both genders were included in study. SPSS version 17 was used for data analysis

Understanding Madrassah Education and Its Impacts. A Case Study of Chach (Attock) region in Pakistan

In recent years, madrassahs and their education systems in many developing countries - and specifically in Pakistan - have attracted much attention from researchers and policymakers at the local and international level. The main focus has been on the reform of madrassahs, their political activism and, more specifically, studies which attempted to investigate their alleged links with militancy. Moreover, madrassah education has been questioned for its relevance to the contemporary needs of individuals and societies. However, despite focusing on many dimensions of madrassahs, few studies have tried to understand madrassah education within the economic, socio-religious and cultural context of Pakistan. A number of publications have reached generalised conclusions about the madrassah education system in Pakistan. Inspired by this, and by adopting qualitative research methods, this study focused on two main research questions:(a) Why do people prefer a madrassah education and what type of factors shape their preference? (b) What are the socio-economic impacts of a madrassah education on individuals and at community level? Researcher conducted a field study of more than six months in the Chach (Attock) region of Pakistan. Different students, parents, madrassah teachers and key informants were interviewed to collect required informations. The findings of the study revealed that different economic backgrounds, parental religious interests, individuals¿ personal religious interests, and social norms and cultural values shape preferences for a madrassah education. Moreover, this study also revealed that there exist various socio-economic impacts of a madrassah education on individuals and at community level. However, a madrassah education has often caused conflict in communities. The study shows that while a madrassah education creates barriers to achieving modern skills and incomes, its social benefits are valuable for those living within socio-cultural constraints in rural areas. Specifically, it enhances the social status and agency of women. The study also shows that madrassah education is an opportunity for those who otherwise would have no other option to study. This study concludes that there is a need to re-think madrassah education within the economic, social, cultural and religious context of Pakistan. This study has practical implications for practitioners, madrassahs and researchers, and it also suggests further research related to madrassah education

Expression and sequence characterization of growth hormone binding protein of Nili-Ravi buffaloes (Bubalus bubalis)

The growth hormone binding protein (GHBP) was isolated from the liver of Nili-Ravi buffaloes (Bubalus bubalis), reverse transcriptase-polymerase chain reaction (RT-PCR) amplified and sequence characterized. RT-PCR analysis demonstrated high degree sequence identities (97.3 to 99.6%) of BbGHBP cDNA with Bos taurus, Ovis aries and Capra hircus. An expression plasmid was constructed for the production of BbGHBP in Escherichia coli BL21 (RIPL) CodonPlus under the control of T7lac promoter. On induction with isopropyl β-D thiogalactopyranoside, the BbGHBP was expressed at levels >30% of the total E. coli proteins. The target protein expressed as inclusion bodies was solubilized in denaturing solution and refolded by step/pulsatile dilution method using cysteine and cystine redox potential. Purification to near homogeniety (>98%) was achieved by ion-exchange chromatography with a recovery yield of 64%. Mass spectrometric analysis of the purified BbGHBP showed a single peak of 30,756 Da. A radioprotein assay evaluated the binding affinity of recombinant BbGHBP with iodinated bovine growth hormone (bGH) which demonstrated active conformation of BbGHBP. These results demonstrate high expression and sequence characterization of BbGHBP in Nili-Ravi buffaloes and provide the basis for the assessment of BbGHBP in other breeds of buffalo.Keywords: Liver, Nili-Ravi buffalo, GHBP, MALDI-TOF mass spectrometry, radioprotein binding assay, refoldin

Firm Size as Moderator to Leverage-Performance Relation: An Emerging Market Review

Present study explored leverage-performance relation while the moderating firm size in developing countries like Pakistan. Data is collected for 304 Pakistani non-financial firms for the period of 2005-2013. It is found that overall leverage-performance relation is negative for all types of firms. However, such losses are more prominent for small size firms. Results also showed that the leverage-performance relation is nonlinear for medium and large size firms.However, in practice these firms are not targeting optimal level and over-leveraging that ultimately decrease their profits.So, financial managers of small size firms should avoid debt financing while for large and medium size firms,managers need to adjust their debt ratio toits optimal level. Keywords: Leverage, Performance, Capital Structure, Moderation, Firm Size JEL code: G32

Mass transfer efficiency of a tall and low plate free area liquid pulsed sieve-plate extraction column

Acknowledgements The authors would like to acknowledge Chakwal group of industries for funding the project. Ms. Madiha, Ms. Zona, Mr. Sohaib, Mr. Abdullah, Mr. Mudassar, and Mr. Salahuddin also deserve our acknowledgements for their assistance in different ways.Peer reviewedPublisher PD

Surgical Outcome of Endoscopic Third Ventriculostomy in Patients Having High ETV Success Score: One-Year Experience at a Tertiary Care Hospital

Background & Objective:  Endoscopic third Ventriculostomy (ETV) is an accepted alternative to VP shunt in patients with obstructive hydrocephalus. We will share our experience and outcome. Materials & Methods:  Thirty consecutive ETV cases performed by a single surgeon during 1 year in patients with an ETV success score of 60 or higher were included in this study. Patients’ demographics, outcomes, and complications are reported. Results:  (60%) were male and 12 (40%) were female. The mean age in our study was 6.1 years ± 9 (mean ± SD). Posterior fossa tumor was the most common etiology in our series (46.6%) followed by aqueductal stenosis (23.3%). Eighty percent of our patients did not experience an ETV failure. The complication rate was 20%. Inadequate ventriculostomy in 6.6% of the patients was the commonest complication. Conclusion:  ETV is safe and effective in patients with high ETV success scores