Spatial Variation in Distribution and Growth Patterns of Old Growth Strip-Bark Pines

Postindustrial rises in CO2 have the potential to confound the interpretation of climatically sensitive tree-ring chronologies. Increased growth rates observed during the 20th century in strip-bark trees have been attributed to CO2 fertilization. Absent in the debate of CO2 effects on tree growth are spatially explicit analyses that examine the proximate mechanisms that lead to changes in rates of tree growth. Twenty-seven pairs of strip-bark and companion entire-bark trees were analyzed in a spatially explicit framework for abiotic environmental correlates. The strip-bark tree locations were not random but correlated to an abiotic proxy for soil moisture. The strip-bark trees showed a characteristic increase in growth rates after about 1875. Furthermore, the difference in growth rates between the strip-bark trees and entire-bark companions increased with increasing soil moisture. A possible mechanism for these findings is that CO2 is affecting water-use efficiency, which in turn affects tree-ring growth. These results point to the importance of accounting for microsite variability in analyzing the potential role of CO2 in governing growth responses

\u3ci\u3eDrosophila\u3c/i\u3e Muller F Elements Maintain a Distinct Set of Genomic Properties Over 40 Million Years of Evolution

The Muller F element (4.2 Mb, ~80 protein-coding genes) is an unusual autosome of Drosophila melanogaster; it is mostly heterochromatic with a low recombination rate. To investigate how these properties impact the evolution of repeats and genes, we manually improved the sequence and annotated the genes on the D. erecta, D. mojavensis, and D. grimshawi F elements and euchromatic domains from the Muller D element. We find that F elements have greater transposon density (25–50%) than euchromatic reference regions (3–11%). Among the F elements, D. grimshawi has the lowest transposon density (particularly DINE-1: 2% vs. 11–27%). F element genes have larger coding spans, more coding exons, larger introns, and lower codon bias. Comparison of the Effective Number of Codons with the Codon Adaptation Index shows that, in contrast to the other species, codon bias in D. grimshawi F element genes can be attributed primarily to selection instead of mutational biases, suggesting that density and types of transposons affect the degree of local heterochromatin formation. F element genes have lower estimated DNA melting temperatures than D element genes, potentially facilitating transcription through heterochromatin. Most F element genes (~90%) have remained on that element, but the F element has smaller syntenic blocks than genome averages (3.4–3.6 vs. 8.4–8.8 genes per block), indicating greater rates of inversion despite lower rates of recombination. Overall, the F element has maintained characteristics that are distinct from other autosomes in the Drosophila lineage, illuminating the constraints imposed by a heterochromatic milieu