Diarrhea and related personal characteristics among Japanese university students studying abroad in intermediate- and low-risk countries

Despite an increasing number of students studying abroad worldwide, evidence about health risks while they are abroad is limited. Diarrhea is considered the most common travelers' illness, which would also apply to students studying abroad. We examined diarrhea and related personal characteristics among Japanese students studying abroad. Japanese university students who participated in short-term study abroad programs between summer 2016 and spring 2018 were targeted (n = 825, 6-38 travel days). Based on a 2-week-risk of diarrhea (passing three or more loose or liquid stools per day) among travelers by country, the destination was separated into intermediate- and low-risk countries. After this stratification, the associations between personal characteristics and diarrhea during the first two weeks of their stay were evaluated using logistic regression models. Among participants in intermediate-risk countries, teenagers, males and those with overseas travel experience were associated with an elevated risk of diarrhea; the odds ratios (95% confidence intervals) were 2.42 (1.08-5.43) for teenagers (vs. twenties), 1.93 (1.08-3.45) for males (vs. females) and 2.37 (1.29-4.33) for those with overseas experience (vs. none). Even restricting an outcome to diarrhea during the first week did not change the results substantially. The same tendency was not observed for those in the low-risk countries. Teenage students, males and those with overseas travel experience should be cautious about diarrhea while studying abroad, specifically in intermediate-risk countries

The 9 and 18 Micron Luminosity Function of Various Types of Galaxies with AKARI: Implication for the Dust Torus Structure of AGN

We present the 9 and 18 micron luminosity functions (LFs) of galaxies at 0.006 < z < 0.8 (with an average redshift of ~ 0.04) using the AKARI mid-infrared all-sky survey catalog. We selected 243 galaxies at 9 micron and 255 galaxies at 18 micron from the Sloan Digital Sky Survey (SDSS) spectroscopy region. These galaxies were then classified by their optical emission lines, such as the line width of H_alpha or by their emission line ratios of [OIII]/H_beta and [NII]/H_alpha into five types: Type 1 active galactic nuclei (AGN) (Type 1); Type 2 AGN (Type 2); low-ionization narrow emission line galaxies (LINER); galaxies with both star formation and narrow-line AGN activity (composite galaxies); and star-forming galaxies (SF). We found that (i) the number density ratio of Type 2 to Type 1 AGNs is 1.73 +/- 0.36, which is larger than a result obtained from the optical LF and (ii) this ratio decreases with increasing 18 micron luminosity.Comment: 38 pages, 19 figures, and 7 tables, accepted for publication in PAS

Relationship between serum calcium or phosphate levels and mortality stratified by parathyroid hormone level: an analysis from the MBD-5D study

Introduction: There is limited evidence about the association between calcium and phosphate levels and mortality stratified by intact parathyroid hormone (iPTH) level. Methods: We investigated whether differences in iPTH level affect the relationship between calcium and phosphate levels and all-cause mortality in hemodialysis patients with secondary hyperparathyroidism (SHPT). Calcium and phosphate levels were categorized as low (< 8.5 mg/dL,  < 4.0 mg/dL), medium (≥ 8.5–< 9.5 mg/dL,  ≥ 4.0–< 7.0 mg/dL), and high (≥ 9.5 mg/dL,  ≥ 7.0 mg/dL), respectively. iPTH levels were grouped into < 300 or ≥ 300 pg/mL. Adjusted incidence rate ratios (aIRRs) were analyzed by weighted Poisson regression. Results: For calcium, patients with higher iPTH (≥ 300 pg/mL) had significantly higher all-cause mortality rates in the high than in the medium category (aIRR 1.99, 95% confidence interval [CI] 1.16–3.42), and tended to have a higher mortality rate in the low category (aIRR 2.04, 95% CI 0.94–4.42). Patients with lower iPTH (< 300 pg/mL) had higher mortality rates in the high than in the medium category (aIRR 1.65, 95% CI 1.39–1.96). For phosphate, the mortality rate was significantly higher in the high than in the medium category in patients with higher and lower iPTH (aIRR 3.23, 95% CI 1.63–6.39 for iPTH ≥ 300 pg/mL; aIRR 1.58, 95% CI 1.06–2.36 for iPTH < 300 pg/mL). Conclusion: High calcium and phosphate levels were associated with increased risk of mortality irrespective of iPTH level

SGLT5 Reabsorbs Fructose in the Kidney but Its Deficiency Paradoxically Exacerbates Hepatic Steatosis Induced by Fructose

Although excessive fructose intake is epidemiologically linked with dyslipidemia, obesity, and diabetes, the mechanisms regulating plasma fructose are not well known. Cells transfected with sodium/glucose cotransporter 5 (SGLT5), which is expressed exclusively in the kidney, transport fructose in vitro; however, the physiological role of this transporter in fructose metabolism remains unclear. To determine whether SGLT5 functions as a fructose transporter in vivo, we established a line of mice lacking the gene encoding SGLT5. Sodium-dependent fructose uptake disappeared in renal brush border membrane vesicles from SGLT5-deficient mice, and the increased urinary fructose in SGLT5-deficient mice indicated that SGLT5 was the major fructose reabsorption transporter in the kidney. From this, we hypothesized that urinary fructose excretion induced by SGLT5 deficiency would ameliorate fructose-induced hepatic steatosis. To test this hypothesis we compared SGLT5-deficient mice with wild-type mice under conditions of long-term fructose consumption. Paradoxically, however, fructose-induced hepatic steatosis was exacerbated in the SGLT5-deficient mice, and the massive urinary fructose excretion was accompanied by reduced levels of plasma triglycerides and epididymal fat but fasting hyperinsulinemia compared with fructose-fed wild-type mice. There was no difference in food consumption, water intake, or plasma fructose between the two types of mice. No compensatory effect by other transporters reportedly involved in fructose uptake in the liver and kidney were indicated at the mRNA level. These surprising findings indicated a previously unrecognized link through SGLT5 between renal fructose reabsorption and hepatic lipid metabolism

Highly Potent GalNAc-Conjugated Tiny LNA Anti-miRNA-122 Antisense Oligonucleotides

The development of clinically relevant anti-microRNA antisense oligonucleotides (anti-miRNA ASOs) remains a major challenge. One promising configuration of anti-miRNA ASOs called “tiny LNA (tiny Locked Nucleic Acid)” is an unusually small (~8-mer), highly chemically modified anti-miRNA ASO with high activity and specificity. Within this platform, we achieved a great enhancement of the in vivo activity of miRNA-122-targeting tiny LNA by developing a series of N-acetylgalactosamine (GalNAc)-conjugated tiny LNAs. Specifically, the median effective dose (ED50) of the most potent construct, tL-5G3, was estimated to be ~12 nmol/kg, which is ~300–500 times more potent than the original unconjugated tiny LNA. Through in vivo/ex vivo imaging studies, we have confirmed that the major advantage of GalNAc over tiny LNAs can be ascribed to the improvement of their originally poor pharmacokinetics. We also showed that the GalNAc ligand should be introduced into its 5′ terminus rather than its 3′ end via a biolabile phosphodiester bond. This result suggests that tiny LNA can unexpectedly be recognized by endogenous nucleases and is required to be digested to liberate the parent tiny LNA at an appropriate time in the body. We believe that our strategy will pave the way for the clinical application of miRNA-targeting small ASO therapy

エゾシカ CERVUS NIPPON YESOENSIS ノ ミトコンドリア DNA エンキ ハイレツ ノ カイセキ : 12SRRNAイデンシ COX II イデンシ オヨビ D-LOOP リョウイキ

エゾシカ(Cervus nippon yesoensis)を有効に活用・保存するためには,基礎的な遺伝的情報を蓄積する必要がある。我々は北海道産エゾシカのミトコンドリアDNA(mtDNA)の12SrRNA遺伝子(957bp),COX II遺伝子(687bp)およびD-loop領域(1,110bp)のDNA配列を解析し,他のシカ科(サンバー,Cervus unicolor ; アカシカ,Cervus elaphus ; タイワンキョン,Muntiacus reevesi ; オジロジカ,Odocoi-leus virginianus ; オグロジカ,Odocoileus hemionus他)動物との比較を行った。12SrRNA遺伝子について解析したところ,調べたCervus属3種はクラスターを形成し,エゾシカはサンバーおよびアカシカに対してそれぞれ2.9および3.5%の塩基置換度を示した。系統樹では,エゾシカとサンバーが最も近く,次いでアカシカが結合した。そのCervus属にMuntiacus属が結合し,Odocoileus属よりも近い関係にあった。COX II遺伝子の解析から,エゾシカとサンバーとの塩基置換度は4.2%であり,またエゾシカとオジロジカとは11.8%であった。系統樹では,12SrRNA遺伝子と同様にエゾシカとサンバーが最も近く,次いでオジロジカが結合した。D-loop領域の解析から,エゾシカと岩手県産のホンシュウジカ(C.n. centralis)との塩基置換度は5.2%と低い値を示した。しかし,山口県産ホンシュウジカおよびツシマジカ(C.n. pulchellus対馬列島産)における塩基置換度は岩手県産のホンシュウジカに比べて高かった(16.6～16.8%)。系統樹では,本邦におけるニホンジカが南北の両グループに分かれ,アジアのニホンジカ,アカシカおよびサンバーは,南グループのニホンジカに近い関係にあった。Accumulation of genetic information is important in order to efficiently utilize and preserve the Yeso Sika deer (Cervus nippon yesoensis). We analyzed DNA sequences of the 12SrRNA gene, Cytochrome c Oxidase II (COX II) gene and the D-loop region of the mitochondrial DNA in the Yeso Sika deer. The sequence divergence of mtDNA was compared in pairs between other Cervidae (Cervus unicolor, Cervus elaphus, Muntiacus reevesi, Odocoileus virginianus, Odocoileus hemionus, etc ). Moreover, phylogenetic analysis was also done between them. The complete sequence of the 12SrRNA gene of the Yeso Sika deer was 957bp, and the sequence divergence between the Yeso Sika deer and Cervus unicolor (Samber) or Cervus elaphus (Red deer) was low in value at 2.9 and 3.5%, respectively. In the phylogenetic tree, the Yeso Sika deer and the Samber were joined first, and following the Red deer was connected to them. Finally, Muntiacus reevesi (Reeves\u27muntjac) was connected to the genus Cervus. As a result of the phylogenetic analysis, we see that the Reeves\u27 muntjac had a closer relationship to the genus Cervus than the genus Odocoileus. The complete sequence of the COX II gene in the Yeso Sika deer was 687bp, and the sequence divergence between the Yeso Sika deer and the Samber or Odocoileus virginianus (White-tailed deer) was 4.2 and 11.8%, respectively. In the phylogenetic tree of the COX II gene, the Yeso Sika deer and the Samber were most closely related as well as the 12SrRNA gene. The next closest relationship was of the White-tailed deer and the genus Cervus. The D-loop region of the Yeso Sika deer was 1110bp, and the sequence divergence between the Yeso Sika deer and the Cervus nippon centralis/Gyo1 (from Iwate Prefecture-Honsyu Sika deer) showed the lowest value (5.2%). Furthermore, the sequence divergence of the Honsyu Sika deer/Yma1 (from Yamaguchi Prefecture) and the C.n. pulchellus (Tushima Sika deer ) was high in value (16.6-16.8%). In the phylogenetic tree, the Sika deer in Japan was classified into two groups of North and South. In addition, the Sika deer of Asia, the Red deer and the Samber were close in relationship to the South group of the Japanease Sika deer

Infrared luminosity functions of AKARI Sloan Digital Sky Survey galaxies

By cross-correlating the AKARI all-sky survey in six infrared (IR) bands (9, 18, 65, 90, 140 and 160 μm) with the Sloan Digital Sky Survey (SDSS) galaxies, we identified 2357 IR galaxies with a spectroscopic redshift. This is not just one of the largest samples of local IR galaxies, but AKARI provides crucial far-IR (FIR) bands for accurately measuring the galaxy spectral energy distribution (SED) across the peak of the dust emission at > 100 μ m. By fitting modern IR SED models to the AKARI photometry, we measured the total infrared luminosity (L_(IR)) of individual galaxies. Using this L_(IR), we constructed the luminosity functions (LF) of IR galaxies at a median redshift of z= 0.031. The LF agrees well with that at z= 0.0082 (the Revised Bright Galaxy Sample), showing smooth and continuous evolution towards higher redshift LFs measured in the AKARI North Ecliptic Pole (NEP) deep field. By integrating the IR LF weighted by L_(IR), we measured the local cosmic IR luminosity density of Ω_(IR_= (3.8^(+5.8)_(−1.2)) × 10^8 L_⊙ Mpc^(−3). We separate galaxies into active galactic nuclei (AGN), star-forming galaxies (SFG) and composite by using the [N ii]/Hα versus [O iii]/Hβ line ratios. The fraction of AGN shows a continuous increase with increasing L_(IR) from 25 to 90 per cent at 9 10^(11) L_⊙, coinciding with the break of both the SFG and AGN IR LFs. At L_(IR)≤ 10^(11) L_⊙, SFG dominates IR LFs. Only 1.1 ± 0.1 per cent of Ω_(IR) is produced by luminous infrared galaxies (L_(IR) > 10^(11) L_⊙), and only 0.03 ± 0.01 per cent by ultraluminous infrared galaxies (L_(IR) > 10^(12) L_⊙) in the local Universe. Compared with high-redshift results from the AKARI NEP deep survey, we observed a strong evolution of Ω^(SFG)IR^∝ (1 +z)^(4.1±0.4) and Ω^(AGN)IR^∝ (1+z)^(4.1±0.5). Our results show that all of our measured quantities (IR LFs, L^*, Ω^(AGN)IR, Ω^(SFG)IR) show smooth and steady increase from lower redshift (the Revised Bright Galaxy Sample) to higher redshift (the AKARI NEP deep survey)

Long-term variability of surface nutrient concentrations in the North Pacific

We present the spatial distributions and temporal changes of the long-term variability of surface nutrient concentrations in the North Pacific by using nutrient samples collected by volunteer ships and research vessels from 1961 to 2012. Nutrient samples are optimally interpolated onto 1° × 1° monthly grid boxes. When the Pacific Decadal Oscillation is in its positive phase, nutrient concentrations in the western North Pacific are significantly higher than the climatological means, and those in the eastern North Pacific are significantly lower. When the North Pacific Gyre Oscillation is in its positive phase, nutrient concentrations in the subarctic are significantly higher than the climatological means. The trends of phosphate and silicate averaged over the North Pacific are ?0.012 ± 0.005 μmol l-1 decade-1 and ?0.38 ± 0.13 μmol l-;1 decade-1, whereas the nitrate trend is not significant (0.01 ± 0.13 μmol l-1 decade-1)

Association of dietary fiber intake with subsequent fasting glucose levels and indicators of adiposity in school-age Japanese children

Abstract Objective: To evaluate the relationships of fiber intake with subsequent body mass index SD-score, waist-to-height ratio, and serum fasting glucose levels among school-age Japanese children. Design: A prospective study of school-age Japanese children. Participants were followed from 6–7 to 9–10 years of age (follow-up rate: 92.0%). Fiber intake was assessed using a validated food frequency questionnaire. Serum fasting glucose was measured by a hexokinase enzymatic method. Using a general linear model, the associations between dietary fiber intake at baseline and body mass index SD-score, waist-to-height ratio, and serum levels of fasting glucose at follow-up were evaluated after considering potential confounding factors. Setting: Public elementary schools in a city in Japan Participants: A total of 2,784 students. Results: The estimated means for fasting glucose at 9–10 years of age were 86.45, 85.68, 85.88, and 85.58 mg/dl in the lowest, second, third, and highest quartile of fiber intake at 6–7 years of age, respectively (p= 0.033, trend p= 0.018). Higher fiber intake at 6–7 years of age was associated with lower waist-to-height ratio at 9–10 years of age (trend p= 0.023). The change in fiber intake was inversely associated with concurrent change of body mass index SD-score (trend p= 0.044). Conclusion: These results suggest that dietary fiber intake may be potentially effective to limit excess weight gain and lower glucose levels during childhood

Seaweed intake and blood pressure levels in healthy pre-school Japanese children

<p>Abstract</p> <p>Background</p> <p>Few studies have examined whether dietary factors might affect blood pressure in children. We purposed to investigate whether seaweed intake is associated with blood pressure level among Japanese preschool children.</p> <p>Methods</p> <p>The design of the study was cross-sectional and it was conducted in autumn 2006. Subjects were healthy preschoolers aged 3-6 years in Aichi, Japan. Blood pressure and pulse were measured once by an automated sphygmomanometer, which uses oscillometric methods. Dietary data, including seaweed intake, were assessed using 3-day dietary records covering 2 consecutive weekdays and 1 weekend day. Of a total of 533 children, 459 (86.1 percent) agreed to be enrolled in our study. Finally, blood pressure measurement, complete dietary records and parent-reported height and weight were obtained for 223 boys and 194 girls.</p> <p>Results</p> <p>When we examined Spearman's correlation coefficients, seaweed intake was significantly negatively related to systolic blood pressure in girls (<it>P </it>= 0.008). In the one-way analysis of covariance for blood pressure and pulse after adjustments for age and BMI, the boys with the lowest, middle and highest tertiles of seaweed intake had diastolic blood pressure readings of 62.8, 59.3 and 59.6 mmHg, respectively (<it>P </it>= 0.11, trend <it>P </it>= 0.038). Girls with higher seaweed intake had significantly lower systolic blood pressure readings (102.4, 99.2 and 96.9 mmHg for girls with the lowest, middle and highest tertiles of seaweed intake, respectively; <it>P </it>= 0.037, trend <it>P </it>= 0.030).</p> <p>Conclusion</p> <p>Our study showed that seaweed intake was negatively related to diastolic blood pressure in boys and to systolic blood pressure in girls. This suggests that seaweed might have beneficial effects on blood pressure among children.</p