Ormosils-PVA BOD sensing film based on oxygen luminescence quenching

通过有机改性溶胶凝胶PVA包埋方法固定微生物菌株 ,以氧光化学传感器作为二次传感 ,制备了响应良好的光化学BOD微生物传感器 .在此基础上 ,考察了微生物传感膜的活化浓度、温度、pH、无机盐及部分金属离子对传感器响应的影响 ,并对传感器的重现性、储存稳定性和线性范围进行了考察 ,实验结果表明BOD含量在 0— 10 0mg L浓度范围内与传感器的荧光值成线性关系 ,传感膜的响应活性保持较好 ,具有较长的使用寿命和良好的稳定性An organically modified silicate (ormosil)-PVA as an immobilization material for Hansennula anomala was investigated to develop an optochemical BOD sensor for the determination of BOD. After preconditioning, the BOD sensing film can be steadily used for two months. The linear fluctuant coefficient(R 2) in the ranges of 0—100 mg/L and 0—40 mg/L was 0.9950 and 0.9604, respectively. The effects of activated concentration, temperature, pH, sodium chloride concentration and coexisting metal ions to BOD determination were investigated.国家 8 6 3计划环境与资源领域 2 0 0 1AA6 35 10 0课题;; 国家自然科学基金委员会科研基金资助项目;; 香港研究资助局科研基金资助项

Effect of Oxygen on the Fluorescence of Sol-Gel with Ruthenium Complexes

利用蓝光LED(λmax =4 6 0nm )构建的氧荧光猝灭检测系统 ,考察了不同Ru配合物以甲基硅氧烷(TMOS)和二甲基二甲氧基硅烷 (DiMe DMOS)为共先驱体 ,制备的有机改性溶胶 凝胶 (sol gel)膜内荧光猝灭行为。DiMe DMOS的加入量会影响敏感膜的极性 ,从而影响敏感膜对氧浓度的响应。利用自行构建的装置对水体中氧进行了检测 ,传感膜对氧饱和水溶液测定的相对标准偏差为 1 12 % (n =6 ) ,响应时间为 30s,对氮饱和水溶液测定相对标准偏差为 0 39% (n =6 ) ,响应时间为 90s,敏感膜使用寿命大于 6个月。Different Ru complexes were used in an organically modified sol-gel (ormosil) sensing film to character their quenching behaviors to oxygen. The sensing film with tris-(4,7-diphenyl-1,10-phenanthroline) ruthenium(Ⅱ) [Ru(dpp) 2+ 3] as an indicator was selected because of its characteristics with high quenching efficiency, fast response time, high luminescence intensity and long-term stability. The quenching behavior of the sensing film greatly depended on the content of dimethyldimethoxysilane (DiMe-DMOS) in ormosil because DiMe-DMOS changed the polarity of sensing film. A home-made fluorescence quenching system was set up for oxygen determination based on a blue LED (λ max=460 nm) as an exciting light source. The response time was 30 s from saturated nitrogen to oxygen in water, and 90 s from saturated oxygen to nitrogen.国家自然科学基金委员会与香港研究资助局联合科研基金;; 国家“863”计划海洋领域 (2 0 0 1AA6351 0 0 )课题资助项

Oxygen Sensing Film Based on Organically Modified Sol-gel with Ruthenium Complexes and Their Fluorescent Spectra Studies

本文考察了利用甲基硅氧烷 (TMOS)和二甲基二甲氧基硅烷 (DiMe DMOS)为共先驱体制备的有机改性溶胶 凝胶 (sol gel)氧荧光敏感传感膜 ,比较了 5种不同配体的Ru配合物在不同极性溶剂和sol gel膜内的荧光行为 ,考察了膜极性的改变对Ru化合物荧光光谱的影响 ,以及氧对不同极性有机改性sol gel包埋Ru化合物荧光猝灭的程度。研究结果表明 ,在sol gel膜内 ,Ru化合物的荧光寿命都有不同程度的增加 ,同时对氧的猝灭程度增加。另外膜的极性对氧猝灭的程度也有很大的影响 ,增加膜的疏水性 ,将提高传感膜对氧浓度的响应A new kind of organically modified sol-gel (ormosil) as a matrix for oxygen-sensitive sensor, in which dimethyldimethoxysilane was selected as an organic modifier in the precursor is described. The fluorescent behaviors of different ruthenium complexes in solution and ormosil film were studied. The sensing film with different kind ruthenium complex as an indicator developed in this paper was characterized in terms of efficiently quenched by oxygen. A new ormosil provides a more desirable environment for oxygen response through increasing film hydrophobicity by the content of the nonbridging Si-CH 3 groups has been discussed. The sensing film with tris-(4,7-diphenyl-1,10-phenanthroline) ruthenium(Ⅱ) as an indicator is selected because of its characteristic with high quenching efficiency, fast response time, high luminescence intensity and long-term stability.国家自然科学基金委员会;; 香港研究资助局联合科研基金 (40 0 1 1 61 946);; 国家 863计划海洋领域课题资助 (2 0 0 1AA6351 0 0

Oxygen sensing film based on organically modified sol-gel with ruthenium complexes and their fluorescent spectra studies

A new kind of organically modified sol-gel (ormosil) as a matrix for oxygen-sensitive sensor, in which dimethyldimethoxysilane was selected as an organic modifier. in the precursor is described. The fluorescent behaviors of different ruthenium complexes in solution and ormosil film were studied. The sensing film with different. kind ruthenium complex as an indicator developed in this paper was characterized in terms of efficiently quenched by oxygen. A new ormosil provides a more desirable environment for oxygen response through increasing film hydrophobicity by the content of the nonbridging Si-CH3 groups has been discussed. The sensing film with, tris-(4,7-diphenyl-1,10-phenanthroline) ruthenium(II) as an indicator is selected because of its characteristic with high quenching efficiency, fast response time, high luminescence intensity and long-term stability