Polyphasic analysis of Acidovorax citrulli strains from northeastern Brazil

ABSTRACT Bacterial fruit blotch (BFB) of cucurbit plants is caused by Acidovorax citrulli and represents a serious concern to melon (Cucumis melo L.) growers worldwide, including those in Brazil. Thirty-four A. citrulli strains from different melon production areas of northeastern Brazil were characterized for their virulence on melon fruits and their substrate utilization and molecular profiles. Based on the analysis of BFB severity on melon fruits, the A. citrulli strains were divided into three groups, classified as mildly, moderately or highly virulent. Although host-related groups were not observed, the watermelon and ‘melão-pepino’ strains exhibited only low or moderate virulence on melon fruit. Substrate utilization profiles revealed that 94 % of the 95 tested compounds were used by A. citrulli strains as a carbon source. Overall, based on substrate utilization, low variability was observed with no relationship to host of origin. The formation of one group of A. citrulli strains based on Repetitive Sequence-based PCR (rep-PCR) analysis confirmed the low variability observed in the substrate utilization analyses. Bayesian inference based on the analysis of 23S rDNA partial sequence data resulted in one well-supported clade and clustered the strains with the A. citrulli-type species with high posterior probability support. Based on the markers used, the Brazilian A. citrulli strains belong to a single group, which corresponds to the previously described Group I for this bacterium in the United States

AI in relationship counselling: Evaluating ChatGPT's therapeutic capabilities in providing relationship advice

Recent advancements in AI have led to chatbots, such as ChatGPT, capable of providing therapeutic responses. Early research evaluating chatbots' ability to provide relationship advice and single-session relationship interventions has showed that both laypeople and relationship therapists rate them high on attributed such as empathy and helpfulness. In the present study, 20 participants engaged in single-session relationship intervention with ChatGPT and were interviewed about their experiences. We evaluated the performance of ChatGPT comprising of technical outcomes such as error rate and linguistic accuracy and therapeutic quality such as empathy and therapeutic questioning. The interviews were analysed using reflexive thematic analysis which generated four themes: light at the end of the tunnel; clearing the fog; clinical skills; and therapeutic setting. The analyses of technical and feasibility outcomes, as coded by researchers and perceived by users, show ChatGPT provides realistic single-session intervention with it consistently rated highly on attributes such as therapeutic skills, human-likeness, exploration, and useability, and providing clarity and next steps for users’ relationship problem. Limitations include a poor assessment of risk and reaching collaborative solutions with the participant. This study extends on AI acceptance theories and highlights the potential capabilities of ChatGPT in providing relationship advice and support

Insecure attachment and support-seeking during COVID-19: a sequential mixed methods investigation

The COVID-19 pandemic led many couples to stay at home together with minimal contact with others. As social distancing measures reduced contact with outside households, many partners could seek support only from one another. In two studies using a sequential mixed methods design, we investigated support-seeking behaviors in romantic relationships during COVID-19. In the qualitative study, semi-structured interviews (n = 48) showed differences in how and why partners seek support with an initial consideration of the role of attachment. We identified the following themes: direct support-seeking, indirect support-seeking, (in)dependence, and gender dynamics. In the quantitative study (n = 588), high COVID-19 worry, high attachment anxiety, and low attachment avoidance were associated with more support-seeking. Interestingly, we also found that when COVID-19 worry was high, individuals high in attachment avoidance were more likely to report seeking support. The paper provides a unique insight into the impact of COVID-19 on support-seeking behaviors in romantic relationships