Concerning the molecular weight, shape, and size of polyglucose isolated from HeLa cells

Light-scattering measurements were made on a preparation of polyglucose isolated from HeLa cells. The molecular weight was determined, from a Zimm plot of the data, to be 4.6[middle dot]106. The character of the angular dependence of the particle-scattering factor suggests the shape of the particle in solution with 779 A gyration radius approximates that of a rigid rod, 2700 A in length with a thickness of 50 A. By quantitative electron microscopy, using reference latex spheres, the molecular weight was estimated to be 1.35[middle dot]106. Electron micrographs show the preparation when air-dried on a collodion membrane to be composed of relatively uniform spheroid particles, approx. 300 A in diameter.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/33488/1/0000893.pd

A high-efficiency HeLa cell nuclear transcription extract.

A HeLa cell nuclear transcription extract that is approximately 20 times more efficient than standard HeLa cell transcription extracts was developed. Transcription of the strong adenovirus II major late promoter by this extract results in the synthesis of 1.5-4 molecules of product RNA per molecule of template, indicating that the extract is capable of multiple rounds of initiation. Standard HeLa cell nuclear extracts transcribe closed circular and linear adenovirus major late promoter templates with equal efficiency. In contrast, the new extract exhibits an increase of approximately twofold on transcription of a closed circular, as opposed to a linear, major late promoter template