Helicobacter pylori serology and the diagnosis of H. pylori infection in children

Serological screening accuracy rate may be dependent on clinical and pathological determinants. The aim of this study was to evaluate the accuracy of Hp serology test (Roche Biomedical Lab., Labcorp), In the diagnosis of Hp infection in 121 children who were seen in the Pediatric Gastoenterology Clinic at the Marshall University Joan C. Edwards School of Medicine In Huntington. Positive serology detected children with Hpassociated gastritis with a sensitivity of 51.6%. Positive serology significantly correlated with the degree of gastric inflammation and density of Hp organisms in the gastric mucosa (ANOVA p \u3c 0.001). The Labcorp. Hp-ELISA test had a poor accuracy rate for the detection of Hp-gastritis in children. Gastric biopsies should always be performed to establish the diagnosis of Hp infection in children

A Radio Transient 0.1 pc from Sagittarius A*

We report the discovery of a transient radio source 2.7 arcsec (0.1 pc projected distance) South of the Galactic Center massive black hole, Sagittarius A*. The source flared with a peak of at least 80 mJy in March 2004. The source was resolved by the Very Large Array into two components with a separation of ~0.7 arcsec and characteristic sizes of ~0.2 arcsec. The two components of the source faded with a power-law index of 1.1 +/- 0.1. We detect an upper limit to the proper motion of the Eastern component of ~3 x 10^3 km s^-1 relative to Sgr A*. We detect a proper motion of ~10^4 km s^-1 for the Western component relative to Sgr A*. The transient was also detected at X-ray wavelengths with the Chandra X-ray Observatory and the XMM-Newton telescope and given the designation CXOGC J174540.0-290031. The X-ray source falls in between the two radio components. The maximum luminosity of the X-ray source is ~10^36 erg s^-1, significantly sub-Eddington. The radio jet flux density predicted by the X-ray/radio correlation for X-ray binaries is orders of magnitude less than the measured flux density. We conclude that the radio transient is the result of a bipolar jet originating in a single impulsive event from the X-ray source and interacting with the dense interstellar medium of the Galactic Center.Comment: accepted in ApJ; 24 pages; 8 figure

The Intrinsic Size of Sagittarius A* from 0.35 cm to 6 cm

We present new high-resolution observations of Sagittarius A* at wavelengths of 17.4 to 23.8 cm with the Very Large Array in A configuration with the Pie Town Very Long Baseline Array antenna. We use the measured sizes to calibrate the interstellar scattering law and find that the major axis size of the scattering law is smaller by ~6% than previous estimates. Using the new scattering law, we are able to determine the intrinsic size of Sgr A* at wavelengths from 0.35 cm to 6 cm using existing results from the VLBA. The new law increases the intrinsic size at 0.7 cm by ~20% and <5% at 0.35 cm. The intrinsic size is 13^{+7}_{-3} Schwarzschild radii at 0.35 cm and is proportional to lambda^gamma, where gamma is in the range 1.3 to 1.7.Comment: ApJL, in pres

Suitability of mycorrhiza-defective mutant/wildtype plant pairs (Solanum lycopersicum L. cv Micro-Tom) to address questions in mycorrhizal soil ecology

Despite the importance of arbuscular mycorrhizal fungi (AMF) to ecosystem processes, few experimental tools are available to quantify AMF contributions to process rates. In this study we examine the efficacy of an experimental system consisting of wildtype (WT) and different non-mycorrhizal (Myc−) genotype pairs of tomato (Solanum lycopersicum L.), specifically focusing on cv Micro-Tom. Two conditions necessary to make such a system useful were examined; (1) that the Myc− genotype(s) do not get colonized in a full soil AMF community background, while the WT does, and B) that there are no non-target effects of the Myc− phenotype on soil microbes. We assessed the second condition by growing Myc− genotypes and WT in non-mycorrhizal soil, monitoring plant growth (root, shoot biomass; root length; root diameter size distribution) and soil microbial community structure (PLFA analysis) as indicators of any changes in root tissue quality or rhizodeposition. All tested Myc− genotypes showed a drastically reduced colonization in mycorrhizal soil. However, in non-mycorrhizal soil, M161 had greater root biomass and M20 greater microbial biomass compared to WT. Only one of the Myc− mutants examined fully met the criteria. We conclude that the BC1/WT pair is a powerful experimental system and recommend caution when using Myc− mutants in mycorrhizal ecology

Permanent Occlusion of Feeding Arteries and Draining Veins in Solid Mouse Tumors by Vascular Targeted Photodynamic Therapy (VTP) with Tookad

Antiangiogenic and anti-vascular therapies present intriguing alternatives to cancer therapy. However, despite promising preclinical results and significant delays in tumor progression, none have demonstrated long-term curative features to date. Here, we show that a single treatment session of Tookad-based vascular targeted photodynamic therapy (VTP) promotes permanent arrest of tumor blood supply by rapid occlusion of the tumor feeding arteries (FA) and draining veins (DV), leading to tumor necrosis and eradication within 24–48 h.A mouse earlobe MADB106 tumor model was subjected to Tookad-VTP and monitored by three complementary, non-invasive online imaging techniques: Fluorescent intravital microscopy, Dynamic Light Scattering Imaging and photosensitized MRI. Tookad-VTP led to prompt tumor FA vasodilatation (a mean volume increase of 70%) with a transient increase (60%) in blood-flow rate. Rapid vasoconstriction, simultaneous blood clotting, vessel permeabilization and a sharp decline in the flow rates then followed, culminating in FA occlusion at 63.2 sec±1.5SEM. This blockage was deemed irreversible after 10 minutes of VTP treatment. A decrease in DV blood flow was demonstrated, with a slight lag from FA response, accompanied by frequent changes in flow direction before reaching a complete standstill. In contrast, neighboring, healthy tissue vessels of similar sizes remained intact and functional after Tookad-VTP.Tookad-VTP selectively targets the tumor feeding and draining vessels. To the best of our knowledge, this is the first mono-therapeutic modality that primarily aims at the larger tumor vessels and leads to high cure rates, both in the preclinical and clinical arenas

Cardiac Arrest Disrupts Caspase-1 and Patterns of Inflammatory Mediators Differently in Skin and Muscle Following Localized Tissue Injury in Rats: Insights from Data-Driven Modeling

Background: Trauma often co-occurs with cardiac arrest and hemorrhagic shock. Skin and muscle injuries often lead to significant inflammation in the affected tissue. The primary mechanism by which inflammation is initiated, sustained, and terminated is cytokine-mediated immune signaling, but this signaling can be altered by cardiac arrest. The complexity and context sensitivity of immune signaling in general has stymied a clear understanding of these signaling dynamics. Methodology/Principal findings: We hypothesized that advanced numerical and biological function analysis methods would help elucidate the inflammatory response to skin and muscle wounds in rats, both with and without concomitant shock. Based on multiplexed analysis of inflammatory mediators, we discerned a differential interleukin (IL)-1α and IL-18 signature in skin vs. muscle, which was suggestive of inflammasome activation in the skin. Immunoblotting revealed caspase-1 activation in skin but not muscle. Notably, IL-1α and IL-18, along with caspase-1, were greatly elevated in the skin following cardiac arrest, consistent with differential inflammasome activation. Conclusions/Significance: Tissue-specific activation of Caspase-1 and the NLRP3 inflammasome appear to be key factors in determining the type and severity of the inflammatory response to tissue injury, especially in the presence of severe shock, as suggested via data-driven modeling

Allocation under dictatorship : research in Stalin’s archives

We survey recent research on the Soviet economy in the state, party, and military archives of the Stalin era. The archives have provided rich new evidence on the economic arrangements of a command system under a powerful dictator including Stalin’s role in the making of the economic system and economic policy, Stalin’s accumulation objectives and the constraints that limited his power to achieve them, the limits to administrative allocation, the information flows and incentives that governed the behavior of economic managers, the scope and significance of corruption and market-oriented behavior, and the prospects for economic reform

Hydration interactions: aqueous solvent effects in electric double layers

A model for ionic solutions with an attractive short-range pair interaction between the ions is presented. The short-range interaction is accounted for by adding a quadratic non-local term to the Poisson-Boltzmann free energy. The model is used to study solvent effects in a planar electric double layer. The counter-ion density is found to increase near the charged surface, as compared with the Poisson-Boltzmann theory, and to decrease at larger distances. The ion density profile is studied analytically in the case where the ion distribution near the plate is dominated only by counter-ions. Further away from the plate the density distribution can be described using a Poisson-Boltzmann theory with an effective surface charge that is smaller than the actual one.Comment: 11 Figures in 13 files + LaTex file. 20 pages. Accepted to Phys. Rev. E. Corrected typos and reference