57,251 research outputs found
Hawking temperature from scattering off the charged 2D black hole
The charged 2D black hole is visualized as presenting an potential barrier
to on-coming tachyon wave. Since this takes the complicated
form, an approximate form is used for scattering analysis. We
calculate the reflection and transmission coefficients for scattering of
tachyon off the charged 2D black hole. The Hawking temperature is also derived
from the reflection coefficient by Bogoliubov transformation. In the limit of
, we recover the Hawking temperature of the 2D dilaton black hole.Comment: 12 pages 3 figures, RevTeX, to obtain figures contact author
([email protected]
The Yersinia enterocolitica Ysa type III secretion system is expressed during infections both in vitro and in vivo.
Yersinia enterocolitica biovar 1B maintains two type III secretion systems (T3SS) that are involved in pathogenesis, the plasmid encoded Ysc T3SS and the chromosomally encoded Ysa T3SS. In vitro, the Ysa T3SS has been shown to be expressed only at 26°C in a high-nutrient medium containing an exceptionally high concentration of salt - an artificial condition that provides no clear insight on the nature of signal that Y. enterocolitica responds to in a host. However, previous research has indicated that the Ysa system plays a role in the colonization of gastrointestinal tissues of mice. In this study, a series of Ysa promoter fusions to green fluorescent protein gene (gfp) were created to analyze the expression of this T3SS during infection. Using reporter strains, infections were carried out in vitro using HeLa cells and in vivo using the mouse model of yersiniosis. Expression of green fluorescent protein (GFP) was measured from the promoters of yspP (encoding a secreted effector protein) and orf6 (encoding a structural component of the T3SS apparatus) in vitro and in vivo. During the infection of HeLa cells GFP intensity was measured by fluorescence microscopy, while during murine infections GFP expression in tissues was measured by flow cytometry. These approaches, combined with quantification of yspP mRNA transcripts by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), demonstrate that the Ysa system is expressed in vitro in a contact-dependent manner, and is expressed in vivo during infection of mice
Initial Experiences of Building Secure Access to Patient Confidential Data via the Internet
A project to enable health care professionals (GPs, practice nurses and diabetes nurse specialists) to access, via the Internet, confidential patient data held on a secondary care (hospital) diabetes information system, has been implemented. We describe the application that we chose to distribute (a diabetes register); the security mechanisms we used to protect the data (a public key infrastructure with strong encryption and digitally signed messages, plus a firewall); the reasons for the implementation decisions we made; the validation testing that we performed and the preliminary results of the pilot implementation
Orientational Melting in Carbon Nanotube Ropes
Using Monte Carlo simulations, we investigate the possibility of an
orientational melting transition within a "rope" of (10,10) carbon nanotubes.
When twisting nanotubes bundle up during the synthesis, orientational
dislocations or twistons arise from the competition between the anisotropic
inter-tube interactions, which tend to align neighboring tubes, and the torsion
rigidity that tends to keep individual tubes straight. We map the energetics of
a rope containing twistons onto a lattice gas model and find that the onset of
a free "diffusion" of twistons, corresponding to orientational melting, occurs
at T_OM > 160 K.Comment: 4 page LaTeX file with 3 figures (10 PostScript files
A model for the screen printing of Newtonian fluids
A preliminary investigation into aspects of the off-contact screen-printing process is presented. A mathematical model for the printing of a thin film of Newtonian fluid is proposed, in which the screen is modelled as a permeable membrane, and the entire region above and below the screen is flooded. By drawing upon widely used industrial circuit printing practices, the distinguished limit of greatest interest to this industry is identified. Numerical and asymptotic solutions of this distinguished limit are presented that reproduce many of the features observed in industrial screen-printing
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