Perancangan Interior Ruang Kerja Redaksi Kantor Tempo — Jakarta

Untuk menjalankan tata usaha, kehadiran kantor sangatlah penting bagi perusahaan. Terlebih bagi perusahaan Tempo Inti Media yang bergerak dalam bidang industri media dan percetakan. Perusahaan Tempo Inti Media yang sebelumnya belum mempunyai kantor tetap, pada akhirnya mampu membangun kantor baru di Palmerah Barat, Jakarta Selatan. Sebagai perusahaan yang berkecimpung dalam dunia jurnalistik, mereka dituntut untuk bekerja cepat dan akurat dalam mengolah berita. Dengan jumlah karyawan yang banyak dengan bermacam jenis pekerjaan, mereka diharapkan mampu bersinergi satu dengan lainnya dalam satu gedung yang baru. Oleh karena itu, perancangan interior kantor redaksi Tempo berfokus pada pemunculan identitas perusahaan, perancangan interior dengan menyesuaikan intensitas penggunaan ruang dan alur kerja keredaksian. Kata kunci: Kantor, Jurnalistik, Indentitas, Intensitas, Alur Kerj

Nitrate and the origin of saliva influence composition and short chain fatty acid production of oral microcosms

Nitrate is emerging as a possible health benefactor. Especially the microbial conversion of nitrate to nitrite in the oral cavity and the subsequent conversion to nitric oxide in the stomach are of interest in this regard. Yet, how nitrate influences the composition and biochemistry of the oral ecosystem is not fully understood. To investigate the effect of nitrate on oral ecology, we performed a 4-week experiment using the multiplaque artificial mouth (MAM) biofilm model. This model was inoculated with stimulated saliva of two healthy donors. Half of the microcosms (n = 4) received a constant supply of nitrate, while the other half functioned as control (n = 4). Additionally, all microcosms received a nitrate and sucrose pulse, each week, on separate days to measure nitrate reduction and acid formation. The bacterial composition of the microcosms was determined by 16S rDNA sequencing. The origin of the saliva (i.e., donor) showed to be the strongest determinant for the development of the microcosms. The supplementation of nitrate was related to a relatively high abundance of Neisseria in the microcosms of both donors, while Veillonella was highly abundant in the nitrate-supplemented microcosms of only one of the donors. The lactate concentration after sucrose addition was similarly high in all microcosms, irrespective of treatment or donor, while the concentration of butyrate was lower after nitrate addition in the nitrate-receiving microcosms. In conclusion, nitrate influences the composition and biochemistry of oral microcosms, although the result is strongly dependent on the inoculum

Evidence for trans-cis isomerization of the p-coumaric acid chromophore as the photochemical basis of the photocycle of photoactive yellow protein

AbstractAnalysis of the chromophore p-courmaix acid, extracted from the ground state and the long-lived blue-shifted photocycle intermediate of photoactive yellow protein, shows that the chromophore is reversibly converted from the trans to the cis configuration, while progressing through the photocycle. The detection of the trans and cis isomers was carried out by high performance capillary zone electrophoresis and further substained by 1H NMR spectroscopy. The data presented here establish the photo-isomerization of the vinyl double bond in the chromophore yellow protein, a eubacterial photosensory protein. A similar isomerization process occurs in the structurally very different sensory rhodopsins, offering an explanation for the strong spectroscopic similarities between photoactive yellow protein and the sensory rhodopsins. This is the first demonstration of light-induced isomerization of a chromophore double bond as the photochemical basis for photosensing in the domain of Bacteria