Разработка системы управления электротехнологической установкой на основе беспроводного интерфейса

Развитие микроконтроллерной техники дает возможность создать беспроводное устройство управления ЭТУ, которое позволило бы решить ряд проблем, возникающих в проводных системах, и сделало бы работу оператора более комфортной и безопасной. Объектом исследования является система беспроводного управления макетом электротехнологической установки (ЭТУ). Цель работы – разработка беспроводной системы управления макетом ЭТУ. В результате исследования была разработана работоспособная система управления ЭТУ состоящая из интерфейса управления и макета ЭТУ.The development of microcontroller technology makes it possible to create a wireless control device for an electrical installation, which would solve a number of problems arising in wired systems, and would make the operator's work more comfortable and safe. The object of the research is the system of wireless control of the layout of the electrotechnological installation of the electrotechnical installation. The purpose of the work is to develop a wireless control system for the layout of an electrotechnical installation. As a result of the study, a workable control system for an electrical installation was developed, consisting of a control interface and a model for an electrical installation

A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale

In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is however critical both for basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale of resolution suitable for comprehensive, brain-wide coverage, using injections of tracers or viral vectors. We detail the scientific and medical rationale and briefly review existing knowledge and experimental techniques. We define a set of desiderata, including brain-wide coverage; validated and extensible experimental techniques suitable for standardization and automation; centralized, open access data repository; compatibility with existing resources, and tractability with current informatics technology. We discuss a hypothetical but tractable plan for mouse, additional efforts for the macaque, and technique development for human. We estimate that the mouse connectivity project could be completed within five years with a comparatively modest budget.Comment: 41 page

Fast extraction of neuron morphologies from large-scale SBFSEM image stacks

Neuron morphology is frequently used to classify cell-types in the mammalian cortex. Apart from the shape of the soma and the axonal projections, morphological classification is largely defined by the dendrites of a neuron and their subcellular compartments, referred to as dendritic spines. The dimensions of a neuron’s dendritic compartment, including its spines, is also a major determinant of the passive and active electrical excitability of dendrites. Furthermore, the dimensions of dendritic branches and spines change during postnatal development and, possibly, following some types of neuronal activity patterns, changes depending on the activity of a neuron. Due to their small size, accurate quantitation of spine number and structure is difficult to achieve (Larkman, J Comp Neurol 306:332, 1991). Here we follow an analysis approach using high-resolution EM techniques. Serial block-face scanning electron microscopy (SBFSEM) enables automated imaging of large specimen volumes at high resolution. The large data sets generated by this technique make manual reconstruction of neuronal structure laborious. Here we present NeuroStruct, a reconstruction environment developed for fast and automated analysis of large SBFSEM data sets containing individual stained neurons using optimized algorithms for CPU and GPU hardware. NeuroStruct is based on 3D operators and integrates image information from image stacks of individual neurons filled with biocytin and stained with osmium tetroxide. The focus of the presented work is the reconstruction of dendritic branches with detailed representation of spines. NeuroStruct delivers both a 3D surface model of the reconstructed structures and a 1D geometrical model corresponding to the skeleton of the reconstructed structures. Both representations are a prerequisite for analysis of morphological characteristics and simulation signalling within a neuron that capture the influence of spines

Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 Å

Photosystem II is the site of photosynthetic water oxidation and contains 20 subunits with a total molecular mass of 350 kDa. The structure of photosystem II has been reported at resolutions from 3.8 to 2.9 angstrom. These resolutions have provided much information on the arrangement of protein subunits and cofactors but are insufficient to reveal the detailed structure of the catalytic centre of water splitting. Here we report the crystal structure of photosystem II at a resolution of 1.9 angstrom. From our electron density map, we located all of the metal atoms of the Mn(4)CaO(5) cluster, together with all of their ligands. We found that five oxygen atoms served as oxo bridges linking the five metal atoms, and that four water molecules were bound to the Mn(4)CaO(5) cluster; some of them may therefore serve as substrates for dioxygen formation. We identified more than 1,300 water molecules in each photosystem II monomer. Some of them formed extensive hydrogen-bonding networks that may serve as channels for protons, water or oxygen molecules. The determination of the high-resolution structure of photosystem II will allow us to analyse and understand its functions in great detail

Abstracts from the 8th International Conference on cGMP Generators, Effectors and Therapeutic Implications

This work was supported by a restricted research grant of Bayer AG

Über den Einfluß der durch Druckzerstörung erzeugten Gitterfehlstellen auf die Lumineszenz von ZnS-Phosphoren

Die quantitativen Gesetzmäßigkeiten des Rückganges der Lumineszenzausbeute von ZnS-Cu mit zunehmendem Zermörserungsgrad (Druckzerstörungsgrad) werden untersucht und auf der Basis des Bändermodelles der Kristallphosphore theoretisch gedeutet. Als Maß für die Druckzerstörung wird eine optisch meßbareGröße eingeführt, die der Zahl der erzeugten Gitterfehlstellen proportional ist. Mit zunehmendem Druckzerstörungsgrad tritt eine — einer monotonen Funktion folgende — Abnahme der Lumineszenzausbeute ein, die von einer Veränderung der Reaktionsordnung für Elektronenübergänge in den Phosphoren begleitet wird. Die Temperaturabhängigkeit der durch Druckzerstörung erzeugten strahlungslosen Elektronenübergänge ist außerordentlich gering. Aus den Messungen folgt die Wesensgleichheit von Druck- undα-Zerstörung. Unter der Annahme, daß den durch Druckzerstörung hervorgerufenen Gitterfehlstellen diskrete metastabile Terme im Bändermodell entsprechen, über die monomolekulare strahlungslose Elektronenübergänge stattfinden, können die experimentellen Ergebnisse quantitativ gedeutet werden