Stac Proteins Suppress Ca2+-Dependent Inactivation of Neuronal L-type Ca2+ Channels

Stac protein (named for its SH3-and cysteine-rich domains) was first identified in brain 20 years ago and is currently known to have three isoforms. Stac2, Stac1, and Stac3 transcripts are found at high, modest, and very low levels, respectively, in the cerebellum and forebrain, but their neuronal functions have been little investigated. Here, we tested the effects of Stac proteins on neuronal, high-voltage-activated Ca2+ channels. Overexpression of the three Stac isoforms eliminated Ca2+-dependent inactivation (CDI) ofL-type current in rat neonatal hippocampal neurons (sex unknown), but not CDI of non-L-type current. Using heterologous expression in tsA201 cells (together with β and α2-δ1 auxiliary subunits), we found that CDI for CaV1.2 and CaV1.3 (the predominant, neuronalL-type Ca2+ channels) was suppressed by all three Stac isoforms, whereas CDI for the P/Q channel, CaV2.1, was not. For CaV1.2, the inhibition of CDI by the Stac proteins appeared to involve their direct interaction with the channel’s C terminus. Within the Stac proteins, a weakly conserved segment containing ~100 residues and linking the structurally conserved PKC C1 and SH3_1 domains was sufficient to fully suppress CDI. The presence of CDI forL-type current in control neonatal neurons raised the possibility that endogenous Stac levels are low in these neurons and Western blotting indicated that the expression of Stac2 was substantially increased in adult forebrain and cerebellum compared with neonate. Together, our results indicate that one likely function of neuronal Stac proteins is to tune Ca2+ entry via neuronal L-type channels. © 2018 the authors

Charge Symmetry Violation Corrections to Determination of the Weinberg Angle in Neutrino Reactions

We show that the correction to the Paschos-Wolfenstein relation associated with charge symmetry violation in the valence quark distributions is essentially model independent. It is proportional to a ratio of quark momenta that is independent of Q^2. This result provides a natural explanation of the surprisingly good agreement found between our earlier estimates within several different models. When applied to the recent NuTeV measurement, this effect significantly reduces the discrepancy with other determinations of the Weinberg angle.Comment: 7 pages, no figures; expanded discussion of N.ne.Z correction

Charge Symmetry Breaking in the Valence Quark Distributions of the Nucleon

Using a quark model, we study the effect of charge symmetry breaking on the valence quark distributions of the nucleon. The effect due to quark mass differences and the Coulomb interaction of the electrically charged quarks is calculated and, in contrast to recent claims, found to be small. In addition, we investigate the effect of charge symmetry breaking in the confining interaction, and in the perturbative evolution equations used to relate the quark model distributions to experiment. We find that both these effects are small, and that the strong charge symmetry breaking effect included in the scalar confining interactions may be distinguishable from that generated by quark mass differences.Comment: 10 pages, LaTEX, 5 Postscript figure

Charge symmetry violation in the parton distributions of the nucleon

We point out that charge symmetry violation in both the valence and sea quark distributions of the nucleon has a non-perturbative source. We calculate this non-perturbative charge symmetry violation using the meson cloud model, which has earlier been successfully applied to both the study of SU(2) flavour asymmetry in the nucleon sea and quark-antiquark asymmetry in the nucleon. We find that the charge symmetry violation in the valence quark distribution is well below 1%, which is consistent with most low energy tests but significantly smaller than the quark model prediction about 5%-10%. Our prediction for the charge symmetry violation in the sea quark distribution is also much smaller than the quark model calculation.Comment: RevTex, 26 pages, 6 PostScript figure

Translating Learners, Researchers, and Qualitative Approaches through Investigations of Students’ Experiences in School

This article uses the conceptual framework offered by ‘translation’ to argue for transforming students into authorities and agents in research on educational practice. Drawing on various definitions of translation and highlighting the influence of recent feminist perspectives on translation studies, the article presents two cases that illustrate how learners can be translated into co-researchers of educational experiences, researchers translated into partners with students in making meaning through the research process, and qualitative research’s approaches and modes of presenting findings translated into new versions of those processes and products

Shadowing in neutrino deep inelastic scattering and the determination of the strange quark distribution

We discuss shadowing corrections to the structure function $F_2$ in neutrino deep-inelastic scattering on heavy nuclear targets. In particular, we examine the role played by shadowing in the comparison of the structure functions $F_2$ measured in neutrino and muon deep inelastic scattering. The importance of shadowing corrections in the determination of the strange quark distributions is explained.Comment: 22 pages, 7 figure

Flavor and Charge Symmetry in the Parton Distributions of the Nucleon

Recent calculations of charge symmetry violation(CSV) in the valence quark distributions of the nucleon have revealed that the dominant symmetry breaking contribution comes from the mass associated with the spectator quark system.Assuming that the change in the spectator mass can be treated perturbatively, we derive a model independent expression for the shift in the parton distributions of the nucleon. This result is used to derive a relation between the charge and flavor asymmetric contributions to the valence quark distributions in the proton, and to calculate CSV contributions to the nucleon sea. The CSV contribution to the Gottfried sum rule is also estimated, and found to be small

A Tandem Mass Spectrometry Sequence Database Search Method for Identification of O-Fucosylated Proteins by Mass Spectrometry.

Thrombospondin type 1 repeats (TSRs), small adhesive protein domains with a wide range of functions, are usually modified with O-linked fucose, which may be extended to O-fucose-β1,3-glucose. Collision-induced dissociation (CID) spectra of O-fucosylated peptides cannot be sequenced by standard tandem mass spectrometry (MS/MS) sequence database search engines because O-linked glycans are highly labile in the gas phase and are effectively absent from the CID peptide fragment spectra, resulting in a large mass error. Electron transfer dissociation (ETD) preserves O-linked glycans on peptide fragments, but only a subset of tryptic peptides with low m/ z can be reliably sequenced from ETD spectra compared to CID. Accordingly, studies to date that have used MS to identify O-fucosylated TSRs have required manual interpretation of CID mass spectra even when ETD was also employed. In order to facilitate high-throughput, automatic identification of O-fucosylated peptides from CID spectra, we re-engineered the MS/MS sequence database search engine Comet and the MS data analysis suite Trans-Proteomic Pipeline to enable automated sequencing of peptides exhibiting the neutral losses characteristic of labile O-linked glycans. We used our approach to reanalyze published proteomics data from Plasmodium parasites and identified multiple glycoforms of TSR-containing proteins