Gene expression profiling of common signal transduction pathways affected by rBMSCs/F92A-Cav1 in the lungs of rat with pulmonary arterial hypertension

BACKGROUND: Pulmonary arterial hypertension (PAH) is associated with sustained vasoconstriction, inflammation and suppressed apoptosis of smooth muscle cells. Our previous studies have found that rat bone marrow-derived mesenchymal stem cells (rBMSCs) transduced with a mutant caveolin-1(F92A-Cav1) could enhance endothelial nitric oxide synthase (eNOS) activity and improve pulmonary vascular remodeling, but the potential mechanism is not yet fully explored. The present study was to investigate the gene expression profile upon rBMSCs/F92A-Cav1delivered to PAH rat to evaluate the role of F92A-Cav1 in its regulation. METHODS: PAH was induced with monocrotaline (MCT, 60mg/kg) prior to delivery of lentiviral vector transduced rBMSCs expressing Cav1 or F92A-Cav1. Gene expression profiling was performed using Rat Signal Transduction PathwayFinder array. The expression changes of 84 key genes representing 10 signal transduction pathways in rat following rBMSCs/F92A-Cav1 treatment was examined. RESULTS: Screening with the Rat Signal Transduction PathwayFinder R2 PCR Array system and subsequent western blot, immunohistochemistry or real time PCR analysis revealed that F92A-Cav1 modified rBMSCs can inhibit the inflammation factors (TNF-alpha, Icam1 and C/EBPdelta), pro-proliferation genes (c-Myc, Bcl2a1d, Notch1and Hey2), oxidative stress gene (Hmox1) and activate cell cycle arrested gene Cdkn1a, ameliorating inflammation and inhibiting cell proliferation in PAH rat. CONCLUSION: rBMSCs/F92A-Cav1 inhibits inflammation and cell proliferation by regulating signaling pathways that related to inflammation, proliferation, cell cycle and oxidative stress

Liquid flow spinning mass-manufactured paraffin cored yarn for thermal management and ultra-high protection

Thermal management and ultra-high protection textiles are critical for polar scientists, astronauts and firefighters. Phase change materials (PCMs) effectively retard huge thermal changes, and thermal damage by absorbing or releasing heat during phase transition. However, due to the materials and engineering challenges inherent in PCMs based textiles, commercial PCMs usually suffer with high rigidity, no-breath-ability, easy leakage and abrasion, limiting their potential applications. Herein, we proposed a mass-producible liquid flow spinning (LFS) method, in which molten paraffin is poured into continuous hollow silicon tubes and then wrapped by staple fibers to form paraffin-coated yarns (PCYs) on a friction spinning frame. The obtained PCYs showed enhanced mechanical properties (break strength of 7.80 N, wear resistance of 2000 cycles) due to the novel core-sheath yarn structure. Besides, thanks to the high melting enthalpy (60.967 J/g) of PCYs, the yarns showed the excellent temperature regulating effect. A double-sided joint PCYs fabric (PCYF) is fabricated to study the PCYs performance further, results show that the PCYF can withstand 10,000 cycles of abrasion without breakage and PCMs leakage. Furthermore, owing to the much gaps provided by the stretch fibers and interweaving points, the fabric exhibits good breathability. In particular, compared with commercial PCMs based textiles, our PCYF is superior in thermal protection performance (9 °C lower). The fireproof performance is also excellent as our PCYF can withstand flame temperatures higher than 1142 °C. The PCYs production method provided here could pave the way for human thermal protection textiles