Distinct forms of the ß subunit of GTP-binding regulatory proteins identified by molecular cloning

Two distinct β subunits of guanine nucleotide-binding regulatory proteins have been identified by cDNA cloning and are referred to as β 1 and β 2 subunits. The bovine transducin β subunit (β 1) has been cloned previously. We have now isolated and analyzed cDNA clones that encode the β 2 subunit from bovine adrenal, bovine brain, and a human myeloid leukemia cell line, HL-60. The 340-residue Mr 37,329 β 2 protein is 90% identical with β 1 in predicted amino acid sequence, and it is also organized as a series of repetitive homologous segments. The major mRNA that encodes the bovine β 2 subunit is 1.7 kilobases in length. It is expreβed at lower levels than β 1 subunit mRNA in all tiβues examined. The β 1 and β 2 meβages are expreβed in cloned human cell lines. Hybridization of cDNA probes to bovine DNA showed that β 1 and β 2 are encoded by separate genes. The amino acid sequences for the bovine and human β 2 subunit are identical, as are the amino acid sequences for the bovine and human β 1 subunit. This evolutionary conservation suggests that the two β subunits have different roles in the signal transduction process

Melvin universe as a limit of the C-metric

It is demonstrated that the Melvin universe representing the spacetime with a strong 'homogeneous' electric field can by obtained from the spacetime of two accelerated charged black holes by a suitable limiting procedure. The behavior of various invariantly defined geometrical quantities in this limit is also studied.Comment: 5 pages, no figures [v2: two references added

A study of ignition phenomena of bulk metals by radiant heating

Early research on combustion of metals was motivated by the knowledge of the large heat release and corresponding high temperatures associated with metal-oxygen reactions. The advent of space flight brought about an increased interest in the ignition and combustion of metallic particles as additives in solid rocket propellants. More recently, attention has been given to the flammability properties of bulk, structural metals due to the number of accidental explosions of metal components in high-pressure oxygen systems. The following work represents a preliminary study that is part of a broader research effort aimed at providing further insight into the phenomena of bulk metal combustion by looking at the effects of gravity on the ignition behavior of metals. The scope of this preliminary experimental study includes the use of a non-coherent, continuous radiation ignition source, the measurement of temperature profiles of a variety of metals and a qualitative observation of the ignition phenomena at normal gravity. The specific objectives of the investigation include: (1) a feasibility study of the use of a continuous radiation source for metal ignition; (2) testing and characterization of the ignition behavior of a variety of metals; and (3) building a preliminary experimental database on ignition of metals under normal gravity conditions

When is |C(X x Y)| = |C(X)||C(Y)|?

Sufficient conditions on the Tychonoff spaces X and Y are found that imply that the equation in the title holds. Sufficient conditions on the Tychonoff space X are found that ensure that the equation holds for every Tychonoff space Y . A series of examples (some using rather sophisticated cardinal arithmetic) are given that witness that these results cannot be generalized much

Cytochrome P450 CYP3A in human renal cell cancer

Renal cell cancer is the main malignant tumour of the kidney and has an increasing incidence. This type of tumour has a poor prognosis and shows intrinsic resistance to several anti-cancer drugs. The CYP3A P450 family, which consists of three closely related forms, is involved in the oxidative activation and deactivation of a variety of carcinogens and several anti-cancer drugs. In this study the presence and cellular localization of CYP3A has been investigated using a combination of immunohistochemistry, immunoblotting and reverse transcriptase polymerase chain reaction (RT-PCR) in renal cell cancer and corresponding normal kidney. CYP3A was consistently expressed in both renal call cancer and in normal kidney. In renal cell cancer, CYP3A was localized to tumour cells and in normal kidney the predominant cellular localization of CYP3A was to proximal tubular epithelial cells. RT-PCR showed that both CYP3A5 mRNA and CYP3A7 mRNA were consistently present in both tumour and normal samples, while CYP3A4 mRNA was present in 65% of tumours and 90% of normal samples. This study indicates that individual members of the CYP3A family are expressed in renal cell cancer. The presence of CYP3A in renal cell cancer might be important in the metabolic potentiation as well as the detoxification of chemotherapeutic agents used to renal cancer. © 1999 Cancer Research Campaig

The Decay of Magnetic Fields in Kaluza-Klein Theory

Magnetic fields in five-dimensional Kaluza-Klein theory compactified on a circle correspond to ``twisted'' identifications of five dimensional Minkowski space. We show that a five dimensional generalisation of the Kerr solution can be analytically continued to construct an instanton that gives rise to two possible decay modes of a magnetic field. One decay mode is the generalisation of the ``bubble decay" of the Kaluza-Klein vacuum described by Witten. The other decay mode, rarer for weak fields, corresponds in four dimensions to the creation of monopole-anti-monopole pairs. An instanton for the latter process is already known and is given by the analytic continuation of the \KK\ Ernst metric, which we show is identical to the five dimensional Kerr solution. We use this fact to illuminate further properties of the decay process. It appears that fundamental fermions can eliminate the bubble decay of the magnetic field, while allowing the pair production of Kaluza-Klein monopoles.Comment: 25 pages, one figure. The discussion of fermions has been revised: We show how fundamental fermions can eliminate the bubble-type instability but still allow pair creation of monopole

Dynamic mechanical properties of human brain tissue

Investigators have been studying the mechanical phenomena associated with impact to the head for many years. Several theories on the behavior of the brain during head impact have come from these studies but there has been a notable lack of information on the bulk mechanical properties of the brain which are necessary for the evaluation of these theories. This paper represents an initial attempt at providing such information.The dynamic complex shear modulus of in vitro samples of human brain have been measured. Specimens from eight brains have been subjected to a sinusoidal shear stress input under resonant conditions in an electro-mechanical test device. Tests were conducted to determine the effects of time after death, refrigeration of material and shear strain dependence. A device to measure the dynamic properties of brain in vivo is described and preliminary data on in vivo tests on Rhesus monkeys is presented.The results of the dynamic shear testing on in vitro human brain indicate that the storage modulus G' lies between 6-11 x 103 dyn/cm2, the loss modulus G" lies between 3[middle dot]5-6[middle dot]0 x 103 dyn/cm2 and the loss tangent tan [delta] is in the range 0[middle dot]40-0[middle dot]55.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/32936/1/0000319.pd