A 17-inch Diameter Shock Tube for Studies in Rarefied Gasdynamics

A shock tube for studying problems in rarefied gasdynamics is described. The motivation for operating at low density (to increase the length and time scales of certain interesting flows) and the effect of low density on the performance and design of the shock tube are discussed. In order to guarantee uniform and reproducible shock waves of moderate strength, the configuration of the tube is conventional. However, innovations are introduced (for example in the suspension, the pumping system, and the diaphragm loading and rupturing mechanism) to simplify the operation of the large facility. Care in the design of the tube as a vacuum system has resulted in a leak rate of less than 0.01 μ Hg per hour. A series of shakedown runs at relatively high pressures has shown, for example, that the reproducibility of a given shock Mach number is ±0.6%

Shock Tubes in Rarefied Gas Flow Research

The flow within a shock wave is governed by the relaxation times of the molecular degrees of freedom. Advances in shock-tube design and instrumentation in recent years have made it possible to resolve all the relaxation times including the shortest, corresponding to the translational degrees of freedom. The shock tube thus becomes an important tool for critical experiments in the study of the range of applicability of the Navier-Stokes equations and similar approximations and of the character of solutions of the Boltzmann equation. Significant progress has recently been made in the understanding of the most obvious such problem, the flow within a shock in a monatomic gas. Theory and experiment are now in substantial agreement and the over-all process of energy exchange is understood. Progress has been made in problems connected with shock wave reflection from real walls, but a host of others remain to be studied including surface interaction effects. The extension of this type of shock-tube research to more complicated systems, reacting gases, gas mixtures, and the like has begun and some progress can be reported. Recent experimental progress is illustrated by a number of measurements made in the 6- and 17-in. shock tubes at the California Institute of Technology

Development of Phage-Based Single Chain Fv Antibody Reagents for Detection of Yersinia pestis

detection. by flow cytometry and whole-cell ELISA. strains, whereas phage displayed scFvs were found to be easy to purify/label and remarkably stable. Furthermore direct fluorescent labeling of phage displaying scFv allowed for an easy one-step flow cytometry assay. Slight cross-reactivity was observed when fixed cells were used in ELISA. F1 antigen. We describe implementation of different methods for phage-based immunoassay. Based on the success of these methods and the proven stability of phage, we indicate that the use of phage-displayed, rather than phage-free proteins, might generally overcome the shortcomings of scFv antibodies

Chronicles of Oklahoma

Notes and Documents, Chronicles of Oklahoma, Volume 33, Number 4, Winter 1955-56. It includes documents about official seals of American Indian nations, notes on Doaksville, Choctaw Nation, a correction for an illustration of Billy Bowlegs in a book, the story of Pushmataha, a correction for The Chronicles, historians of the Ottawa, and the nomination of Ollie Magdalene Butler as the Pioneer Woman of Oklahoma of 1955

New Insights into the Structure of (1→3,1→6)-β-D-Glucan Side Chains in the Candida glabrata Cell Wall

β-glucan is a (1→3)-β-linked glucose polymer with (1→6)-β-linked side chains and a major component of fungal cell walls. β-glucans provide structural integrity to the fungal cell wall. The nature of the (1–6)-β-linked side chain structure of fungal (1→3,1→6)-β-D-glucans has been very difficult to elucidate. Herein, we report the first detailed structural characterization of the (1→6)-β-linked side chains of Candida glabrata using high-field NMR. The (1→6)-β-linked side chains have an average length of 4 to 5 repeat units spaced every 21 repeat units along the (1→3)-linked polymer backbone. Computer modeling suggests that the side chains have a bent curve structure that allows for a flexible interconnection with parallel (1→3)-β-D-glucan polymers, and/or as a point of attachment for proteins. Based on these observations we propose new approaches to how (1→6)-β-linked side chains interconnect with neighboring glucan polymers in a manner that maximizes fungal cell wall strength, while also allowing for flexibility, or plasticity

Heuristics for the inversion median problem

Background: The study of genome rearrangements has become a mainstay of phylogenetics and comparative genomics. Fundamental in such a study is the median problem: given three gene arrangements, find a fourth that minimizes the sum of the evolutionary distances between itself and the given three. Many exact algorithms and heuristics have been developped for the inversion median problem, of which the best known is MGR. Results: We present a unifying framework for median heuristics, which enables us to clarify existing strategies and to place them in a partial ordering. Analysis of this framework leads to a new insight: the best strategies continue to refer to the input data rather than just to updated estimates. Using this insight, we develop a new heuristic for inversion medians that uses input data to the end of its computation and leverages our previous work with DCJ medians. Finally, we present the results of extensive experimentation showing that our new heuristic outperforms all others in accuracy and, especially, in running time: the heuristic typically returns solutions within 1 % of optimal and runs in seconds to minutes even on genomes with 25’000 genes—in contrast, MGR can take days on instances of 200 genes and cannot be used beyond 1’000 genes. Conclusions: Finding good rearrangement medians, in particular inversion medians, had long been regarded as the computational bottleneck in whole-genome studies. Our new heuristic for inversion medians, ASM, which dominates all others in our framework, puts that issue to rest by providing near-optimal solutions within seconds to minutes on even the largest genomes

Meiotic Transmission of Drosophila pseudoobscura Chromosomal Arrangements

Drosophila pseudoobscura harbors a rich gene arrangement polymorphism on the third chromosome generated by a series of overlapping paracentric inversions. The arrangements suppress recombination in heterokaryotypic individuals, which allows for the selective maintenance of coadapted gene complexes. Previous mapping experiments used to determine the degree to which recombination is suppressed in gene arrangement heterozygotes produced non-recombinant progeny in non-Mendelian ratios. The deviations from Mendelian expectations could be the result of viability differences between wild and mutant chromosomes, meiotic drive because of achiasmate pairing of homologues in heterokaryotypic females during meiosis, or a combination of both mechanisms. The possibility that the frequencies of the chromosomal arrangements in natural populations are affected by mechanisms other than adaptive selection led us to consider these hypotheses. We performed reciprocal crosses involving both heterozygous males and females to determine if the frequency of the non-recombinant progeny deviates significantly from Mendelian expectations and if the frequencies deviate between reciprocal crosses. We failed to observe non-Mendelian ratios in multiple crosses, and the frequency of the non-recombinant classes differed in only one of five pairs of reciprocal crosses despite sufficient power to detect these differences in all crosses. Our results indicate that deviations from Mendelian expectations in recombination experiments involving the D. pseudoobscura inversion system are most likely due to fitness differences of gene arrangement karyotypes in different environments