28 research outputs found
Effects of Exendin-4 on human adipose tissue inflammation and ECM remodelling
Subjects with type-2 diabetes are typically obese with dysfunctional adipose tissue (AT). Glucagon-like peptide-1 (GLP-1) analogues are routinely used to improve glycaemia. Although, they also aid weight loss that improves AT function, their direct effect on AT function is unclear. To explore GLP-1 analogues’ influence on human AT’s cytokine and extracellular matrix (ECM) regulation, we therefore obtained and treated omental (OMAT) and subcutaneous (SCAT) AT samples with Exendin-4, an agonist of the GLP-1 receptor (GLP-1R)Final publishe
Position-Specific Chemical Modification and Quantitative Proteomics Disclose Protein Orientation Adsorbed on Silica Nanoparticles
We describe a method for determining the orientation
of cytochrome
c, RNase A, and lysozyme on silica nanoparticles (SNPs) using chemical
modification combined with proteolysis-mass spectrometry. The proteins
interacted with SNPs through preferential adsorption sites, which
are dependent on SNP diameter; 4 nm SNPs induce greater structural
stabilization than 15 nm particles, presumably due to greater surface
curvature of the former. These results suggest that nanoparticle size
and protein structure influence protein orientation on SNPs