Health promoting potential of herbal teas and tinctures from Artemisia campestris subsp maritima: from traditional remedies to prospective products

This work explored the biotechnological potential of the medicinal halophyte Artemisia campestris subsp. maritima (dune wormwood) as a source of health promoting commodities. For that purpose, infusions, decoctions and tinctures were prepared from roots and aerial-organs and evaluated for in vitro antioxidant, anti-diabetic and tyrosinase-inhibitory potential, and also for polyphenolic and mineral contents and toxicity. The dune wormwood extracts had high polyphenolic content and several phenolics were identified by ultra-high performance liquid chromatography-photodiode array-mass-spectrometry (UHPLC-PDA-MS). The main compounds were quinic, chlorogenic and caffeic acids, coumarin sulfates and dicaffeoylquinic acids; several of the identified phytoconstituents are here firstly reported in this A. campestris subspecies. Results obtained with this plant's extracts point to nutritional applications as mineral supplementary source, safe for human consumption, as suggested by the moderate to low toxicity of the extracts towards mammalian cell lines. The dune wormwood extracts had in general high antioxidant activity and also the capacity to inhibit a-glucosidase and tyrosinase. In summary, dune wormwood extracts are a significant source of polyphenolic and mineral constituents, antioxidants and a-glucosidase and tyrosinase inhibitors, and thus, relevant for different commercial segments like the pharmaceutical, cosmetic and/or food industries.FCT - Foundation for Science and Technology [CCMAR/Multi/04326/2013]; Portuguese National Budget; FCT [IF/00049/2012, SFRH/BD/94407/2013]; Research Foundation - Flanders (FWO) [12M8315N]info:eu-repo/semantics/publishedVersio

Myrtus communis L. Ellagitannins and Flavonoids Protect Cardiomyoblast Cells against CoCl2 -Induced Hypoxia and H2 O 2 Stress by Improving Oxidative Balance

International audienceThis study evaluates the potential protective effect of Myrtus communis leaves buthanolic fraction (MBF) on CoCl2 and H2 O2 -challenged H9c2 cardiomyoblast cells. The identification of the main compounds by LC-ESITOF-MS was assessed. Among the 23 identified molecules (12 ellagitannins, 8 flavonoids, 1 coumarins, 1 anthocyanin and 1 myrtucommulone), 6 have been described for the first time in M. communis. The MBF displayed high amount of total phenolic (151.09mg of GAE/g of DR), flavonoid and tannin contents (21.81 and 49.54 mg of CE/g of DR, resp.) related to its important antioxidant activity such as DPPH (IC50 = 3.3μg/mL) and ABTS (IC50 = 14μg/mL) tests. Otherwise, exposure of cultured H9C2 cells to CoCl2 -simulated hypoxia or H2 O2 -oxidative stress induced over-activity of superoxide dismutase (over 181 %) and Catalase (171 and 500 %, resp.) and decrease in glutathione peroxidase (≈ 48 %) and excess in MDA (≈ 300 %) level. Pretreatment with MBF, attenuated significantly CoCl2 and H2 O2 challenged cardiomyoblasts injury by restoring cell viability (≈ 100 %), oxidative balance and MDA level. These results indicate that WBF can be used as a functional food to reduce the side effects of the oxidative stress and can be therapeutically effective candidate in cardiac ischemic injuries

Pharm Biol

Frankenia pulverulenta L. (Frankeniaceae) is a medicinal species with carminative, analgesic and antiviral properties. However, phytochemical investigations, antioxidant and neuroprotective capacities of this plant remain unclear. This work assesses the phenolic composition of F. pulverulenta shoot and root and evaluates their antioxidant and neuroprotective capacities. Successive fractionation of F. pulverulenta shoot and root using 6 solvents were used. Antioxidant capacity of these fractions was assessed through four in vitro tests (DPPH, ABTS, Fe-chelating activity and ORAC). Phenolic identification, purification as well as neuroprotective activity of ethyl acetate (EtOAc) fraction and purified molecules were assessed. Among the tested fractions, EtOAc shoot and root fractions possessed considerable phenolic contents (383 and 374 mg GAE/g E, respectively) because of their important ORAC (821 and 1054 mg of TE/g E), DPPH (586 and 750 mg of TE/g) and ABTS (1453 and 1319 mg of TE/g) results. Moreover, gallic acid, quercetin, quercetin galloyl glucoside, trigalloyl hexoside, procyanidin dimers and sulfated flavonoids were identified by LC-DAD-ESI-MS for the first time in this species. The relevant cytoprotective capacity (at 300 μg/mL) against β-amyloid peptide induced toxicity in PC12 cells of EtOAc fractions were corroborated with the chemical composition. In addition, purified molecules were tested for their ORAC and neuroprotective activity. Quercetin showed the best ORAC value (33.55 mmol TE/g polyphenols); nevertheless, procyanidin dimer exhibited an exceptionally efficient neuroprotective activity (100% of viability at 50 μg/mL). These findings suggest that this halophyte is a promising source of antioxidant and neuroprotective molecules for pharmaceutical purposes