Influence of inorganic and organic selenium on number of living mycelial cells and their ultrastructure in culture of Hericium erinaceum (Bull.: Fr. Pers.)

Mycelium of the white-rot fungus (Hericium erinaceum (Bull.: Fr. Pers.) produces polysaccharides showing anticancer and immunostimulating activity. In our previous works, we have shown that organic selenitetriglycerides (Selol) contribute to the increase of biosynthesis of exopolysaccharides (EPS) having antioxidative properties and containing large amounts of selenium. The present work is a study of influence of inorganic and organic form of selenium on viability of H. erinaceum mycelium and on ultrastructural changes taking place during its development in submerged culture. The mycelium was grown on media containing sodium selenite (Na2SeO3), a mixture of Na2SeO3 + Selol2% and on control medium (no selenium added). It was shown that mycelium cultured for 3 days in control conditions on standard media contained almost 100% of living cells, with over 80% after 24 days. Treatment with 100 ppm of Na2SeO3 lowered the number of viable cells to 11.8% and 9.1% after 3 and 24 days, respectively. The addition of 2% Selol caused the amounts of living cells to remain at ca 90%. Apparently, Selol helped the cells to cope with the toxic activity of inorganic selenium ions. The addition of sodium selenite induced degradative changes in cell organelles. Such changes were not observed in the case of Na2SeO3 + Selol mixture, in which case cells contained numerous ribosomes and small lipid bodies