158 research outputs found
Assessment and treatment of chronic work-related pain disorder in an output university rehabilitation setting in the Netherlands
Assessment and treatment of chronic work-related pain disorder in an output university rehabilitation setting in the Netherlands
Treatment of shoulder complaints in general practice:long term results of a randomised, single blind study comparing physiotherapy, manipulation, and corticosteroid injection
Treatment of shoulder complaints in general practice:long term results of a randomised, single blind study comparing physiotherapy, manipulation, and corticosteroid injection
Treatment of shoulder complaints in general practice:long term results of a randomised, single blind study comparing physiotherapy, manipulation, and corticosteroid injection
Treatment of shoulder complaints in general practice:long term results of a randomised, single blind study comparing physiotherapy, manipulation, and corticosteroid injection
GMP manufacturing of Vvax001, a therapeutic anti-HPV vaccine based on recombinant viral particles
Therapeutic vaccination is being explored as a treatment strategy for the treatment of patients with primary or metastatic tumours. We developed a vaccine targeted to Human papillomavirus (HPV)-induced tumours based on recombinant Semliki Forest virus (rSFV) encoding a fusion protein of the E6 and E7 proteins of HPV type 16. To enable a phase I clinical trial with this vaccine, Vvax001, a Good Manufacturing Practice (GMP)-compliant manufacturing process was set up and clinical material was produced. Upstream production of the clinical material resulted in viral titers from 2.4 Γ 107 to 1.3 Γ 109 infectious particles/ mL in the harvest. The total volume of 6.0 liter crude virus was purified in 13 consecutive downstream purification runs. The mean titer after purification was 4.0 Γ 108 infectious particles/ mL and the mean recovery was 19%. Finally, clinical material was filled at a target concentration of 1.25 Γ 108 infectious particles/mL. Release testing included tests for viral titer and virus identity, biological activity, sterility, bacterial endotoxins, adventitious viruses and absence of replication competent virus. The product complied with all specifications and was released for use as an investigational medicinal product. This is the first GMP production process developed for a SFV-based therapeutic vaccine. The vaccine, Vvax001 is targeted to HPV and has shown promising results in preclinical studies. The GMP-produced Vvax001 material met the quality criteria and was of sufficient quantity to enable assessment of its immunogenicity, safety and efficacy in a clinical setting
ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ²ΠΎΠ±ΠΎΠ΄Π½ΡΡ ΠΊΠΎΠ»Π΅Π±Π°Π½ΠΈΠΉ ΠΎΡΡΠΎΡΡΠΎΠΏΠ½ΡΡ ΡΠΈΠ»ΠΈΠ½Π΄ΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΎΠ±ΠΎΠ»ΠΎΡΠ΅ΠΊ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΠΌΠΎΠ΄Π΅Π»Π΅ΠΉ
ΠΠΎΡΠ»ΡΠ΄ΠΆΡΡΡΡΡΡ Π²ΡΠ»ΡΠ½Ρ ΠΊΠΎΠ»ΠΈΠ²Π°Π½Π½Ρ ΠΎΡΡΠΎΡΡΠΎΠΏΠ½ΠΈΡ
ΡΠΈΠ»ΡΠ½Π΄ΡΠΈΡΠ½ΠΈΡ
ΠΎΠ±ΠΎΠ»ΠΎΠ½ΠΎΠΊ ΠΏΡΠΈ ΡΡΠ·Π½ΠΈΡ
Π³ΡΠ°Π½ΠΈΡΠ½ΠΈΡ
ΡΠΌΠΎΠ²Π°Ρ
Π½Π° ΠΊΡΠ°ΡΡ
Π² ΡΡΠΎΡΠ½Π΅Π½ΡΠΉ ΠΏΠΎΡΡΠ°Π½ΠΎΠ²ΡΡ Π· Π·Π°ΡΡΠΎΡΡΠ²Π°Π½Π½ΡΠΌ ΡΠ΅ΠΎΡΡΡ ΠΡΠ½Π΄Π»ΡΠ½Π°βΠ’ΠΈΠΌΠΎΡΠ΅Π½ΠΊΠ° ΡΠ° Π½Π° ΠΎΡΠ½ΠΎΠ²Ρ ΡΡΠΈΠ²ΠΈΠΌΡΡΠ½ΠΎΡ ΡΠ΅ΠΎΡΡΡ ΠΏΡΡΠΆΠ½ΠΎΡΡΡ. ΠΠ»Ρ ΡΠΎΠ·ΡΠ°Ρ
ΡΠ½ΠΊΡ ΡΠ°ΡΡΠΎΡ Π²ΠΈΠΊΠΎΡΠΈΡΡΠΎΠ²ΡΡΡΡΡΡ ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ-Π°Π½Π°Π»ΡΡΠΈΡΠ½ΠΈΠΉ ΠΏΡΠ΄Ρ
ΡΠ΄, ΡΠΊΠΈΠΉ Π±Π°Π·ΡΡΡΡΡΡ Π½Π° Π·Π°ΡΡΠΎΡΡΠ²Π°Π½Π½Ρ ΡΠΏΠ»Π°ΠΉΠ½-Π°ΠΏΡΠΎΠΊΡΠΈΠΌΠ°ΡΡΡ, Π° ΡΠ°ΠΊΠΎΠΆ ΠΌΠ΅ΡΠΎΠ΄Ρ ΠΊΠΎΠ»ΠΎΠΊΠ°ΡΡΡ, Π΄ΠΈΡΠΊΡΠ΅ΡΠ½ΠΎΡ ΠΎΡΡΠΎΠ³ΠΎΠ½Π°Π»ΡΠ·Π°ΡΡΡ ΡΠ°Π·ΠΎΠΌ Π· ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΠΎΠΊΡΠΎΠΊΠΎΠ²ΠΎΠ³ΠΎ ΠΏΠΎΡΡΠΊΡ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΏΠΎΡΡΠ²Π½ΡΠ½Π½Ρ ΡΠ°ΡΡΠΎΡ ΡΠΈΠ»ΡΠ½Π΄ΡΠΈΡΠ½ΠΈΡ
ΠΎΠ±ΠΎΠ»ΠΎΠ½ΠΎΠΊ Π· ΡΡΠ·Π½ΠΈΠΌΠΈ Π³ΡΠ°Π½ΠΈΡΠ½ΠΈΠΌΠΈ ΡΠΌΠΎΠ²Π°ΠΌΠΈ Π½Π° ΡΠΎΡΡΡΡ
, ΠΎΡΡΠΈΠΌΠ°Π½ΠΈΡ
Π² ΡΠ°ΠΌΠΊΠ°Ρ
ΡΡΠ·Π½ΠΈΡ
ΠΌΠΎΠ΄Π΅Π»Π΅ΠΉ.A problem of natural vibrations of orthotropic cylindrical shells under various boundary conditions of its end-faces within the framework of the MindlinβTimoshenko theory and on the basis of 3-D elasticity theory is considered. Using the method of spline-approximation and collocation, the problems are solved by the steady-state numerical method of discrete orthogonalization with incremental search. The comparison of the frequencies of cylindrical shells with different boundary conditions on their ends within various models is performed
Coarsening Dynamics of Crystalline Thin Films
The formation of pyramid-like structures in thin-film growth on substrates
with a quadratic symmetry, e.g., {001} surfaces, is shown to exhibit
anisotropic scaling as there exist two length scales with different time
dependences. Analytical and numerical results indicate that for most
realizations coarsening of mounds is described by an exponent n=0.2357.
However, depending on material parameters, n may lie between 0 (logarithmic
coarsening) and 1/3. In contrast, growth on substrates with triangular
symmetries ({111} surfaces) is dominated by a single length scale and an
exponent n=1/3.Comment: RevTeX, 4 pages, 3 figure
Natuurverkenning 2010-2040 : visies op de ontwikkeling van natuur en landschap
De Natuurverkenning verschijnt in een turbulente tijd waarin natuur en landschap sterk gepolitiseerd zijn. Met de verkenning wil het PBL een bijdrage leveren aan het structureren van het debat over de vernieuwing van het langetermijnbeleid en een impuls geven aan de politieke afwegingen. Nieuw is het gebruik van normatieve toekomstscenarioβs als hulpmiddel om de achterliggende drijfveren voor natuurbeleid te verhelderen
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