Маркетинговая деятельность предприятия малого и среднего бизнеса в Китае – условие выхода на новые сегменты рынка

Объектом исследования является компания Хин Шинг. Предмет исследования – формирование маркетинговой стратегии деятельности компании Хин Шинг. Целью работы является построение стратегии маркетингового развития деятельности предприятия на примере компании Хин Шинг, Китай. В результате исследования были выявлены ключевые направления маркетинговой деятельности предприятия, проанализирована внутренняя среда компании и разработаны дополнительные маркетинговые инструменты, направленные на продвижение продукции.The object of research is the company Hing Shing. Subject of research - the formation of a marketing strategy Hing Shing company. The aim is to build a marketing strategy of development of the enterprise on an example of the company Hing Shing, China. The study identified the key areas of marketing activity of the enterprise, analyze the internal environment of the company and to develop additional marketing tools aimed at the promotion

Two Siblings with a Mutation in CCDC8 Presenting with Mild Short Stature: A Case of 3-M Syndrome

BACKGROUND: Short stature can be caused by mutations in a multitude of different genes. 3-M syndrome is a rare growth disorder marked by severe pre- and postnatal growth retardation along with subtle dysmorphic features. There have only been 2 prior reports of mutations in CCDC8 causing 3-M syndrome. METHODS: Two patients presenting with mild short stature underwent whole exome sequencing. The mutation was confirmed via Sanger sequencing. We compare the clinical characteristics of our 2 patients to patients previously reported with mutations in the same gene. RESULTS: Exome sequencing identified a homozygous frameshift mutation in CCDC8 in both patients. They presented with a much milder phenotype than previously described patients with the same mutation. CONCLUSION: In this study, we report a case of 2 sisters with relatively mild short stature who were found via exome sequencing to carry a previously reported homozygous mutation in CCDC8. These patients expand the anthropometric phenotype of 3-M syndrome and demonstrate the power of exome sequencing in the diagnosis of children with short stature. 3-M syndrome should be considered in children with mild skeletal abnormalities, normal/high growth hormone-IGF axis parameters, and normal intelligence

Intramuscular DNA Vaccination of Juvenile Carp against Spring Viremia of Carp Virus Induces Full Protection and Establishes a Virus-Specific B and T Cell Response

Although spring viremia of carp virus (SVCV) can cause high mortalities in common carp, a commercial vaccine is not available for worldwide use. Here, we report a DNA vaccine based on the expression of the SVCV glycoprotein (G) which, when injected in the muscle even at a single low dose of 0.1 µg DNA/g of fish, confers up to 100% protection against a subsequent bath challenge with SVCV. Importantly, to best validate vaccine efficacy, we also optimized a reliable bath challenge model closely mimicking a natural infection, based on a prolonged exposure of carp to SVCV at 15°C. Using this optimized bath challenge, we showed a strong age-dependent susceptibility of carp to SVCV, with high susceptibility at young age (3 months) and a full resistance at 9 months. We visualized local expression of the G protein and associated early inflammatory response by immunohistochemistry and described changes in the gene expression of pro-inflammatory cytokines, chemokines, and antiviral genes in the muscle of vaccinated fish. Adaptive immune responses were investigated by analyzing neutralizing titers against SVCV in the serum of vaccinated fish and the in vitro proliferation capacity of peripheral SVCV-specific T cells. We show significantly higher serum neutralizing titers and the presence of SVCV-specific T cells in the blood of vaccinated fish, which proliferated upon stimulation with SVCV. Altogether, this is the first study reporting on a protective DNA vaccine against SVCV in carp and the first to provide a detailed characterization of local innate as well as systemic adaptive immune responses elicited upon DNA vaccination that suggest a role not only of B cells but also of T cells in the protection conferred by the SVCV-G DNA vaccine

Mathematically Gifted Adolescents Have Deficiencies in Social Valuation and Mentalization

Many mathematically gifted adolescents are characterized as being indolent, underachieving and unsuccessful despite their high cognitive ability. This is often due to difficulties with social and emotional development. However, research on social and emotional interactions in gifted adolescents has been limited. The purpose of this study was to observe differences in complex social strategic behaviors between gifted and average adolescents of the same age using the repeated Ultimatum Game. Twenty-two gifted adolescents and 24 average adolescents participated in the Ultimatum Game. Two adolescents participate in the game, one as a proposer and the other as a responder. Because of its simplicity, the Ultimatum Game is an apt tool for investigating complex human emotional and cognitive decision-making in an empirical setting. We observed strategic but socially impaired offers from gifted proposers and lower acceptance rates from gifted responders, resulting in lower total earnings in the Ultimatum Game. Thus, our results indicate that mathematically gifted adolescents have deficiencies in social valuation and mentalization

Trade-Off Between Delineation and Lighting on Freeway Interchanges

DTFH61-85-C-00137The objective was to determine whether, with improved delineation, performance at partially lighted interchanges can approach performance under full lighting, particularly in rain. Two field studies were conducted. The first was to determine whether transient visual adaptation (TVA) influences detection on partially lighted interchanges and could interact with lighting. It was shown that TVA occurs under partial lighting and influences detection up to 600 feet from the last luminaire. The second field study was to determine the effect of lighting, weather, and improved delineation on driver performance. Data were obtained on two exits in dry and wet weather under full lighting with baseline delineation and three improved delineation systems. Partial lighting at one exit was with one luminaire, at the other with three luminaires. Findings support the contention that full lighting is superior to partial lighting in ramp speed-related measures. Analysis of delineation effects on ramp and spot speeds and on speed distributions showed few differences under dry conditions. In rain, effects were stronger but were neither large nor consistent enough to recommend improved delineation over the baseline system. Nonstatistical comparison of the results from two sited provided evidence that three-luminaire partial lighting was superior to single-luminaire. Performance on ramp segments downstream of the last luminaire suggested TVA influenced results

Determination of Driver Needs in Work Zones

DOT-FH-11-9549The objectives of the study were: (a) to determine what information drivers need to travel through work zones safely and efficiently; (b) to determine how this information can best be conveyed to the drivers; and (c) to determine where improvements to the present system of work zone traffic control are needed. The study began with an analysis of driver tasks for eight major work zone types: lane closure, shoulder closure, roadside, lane diversion, crossover, temporary detour, detour to alternate routes, and reduced lane width. From this effort, a set of information content needs was identified for each work zone type. A further analytic effort using the principles of the Positive Guidance Procedure and the concept of Decision Sight Distance, resulted in the identification of recommended information presentation locations for the various types of information. These analytic efforts were combined into information requirements which were then evaluated for applicability by exercising each on a series of actual work zones. The requirements were modified where necessary and were then used as the basis for the development of a procedure for the derivation of work zones signing plans. Another aspect of the project involved the evaluation of individual construction-related signs, in which each device was evaluated with respect to several criteria and problems were identified

Atypical STAT5B deficiency, severe short stature and mild immunodeficiency associated with a novel homozygous STAT5B Variant

STAT5B deficiency, a rare autosomal recessive disorder characterized by severe growth hormone insensitivity (GHI) and immunodeficiency, can manifest as fatal pulmonary complications. We describe atypical STAT5B deficiency associated with a novel homozygous frame-shift STAT5B variant [c.1453delG, p.(Asp485Thrfs*29)] identified in a young 17.6 yr old female subject who had severe postnatal growth impairment, biochemistries typical of GHI, an immune profile notable for hypergammaglobulinaemia and elevated B lymphocytes, and lack of pulmonary disease. Marked elevation of serum prolactin and pathologically diagnosed eczema were evident. In reconstitution studies, the STAT5B p.(Asp485Thrfs*29) was expressed although expression was reduced compared to wild-type STAT5B and a previously identified STAT5B p.(Gln368Profs*9) variant. Both truncated STAT5B peptides could not be activated by GH, nor mobilize to the nucleus. We conclude that an intact, func-tional, STAT5B is essential for normal GH-mediated growth, while expressed loss-of-function STAT5B variants may alleviate severe immune and pulmonary issues normally associated with STAT5B deficiency.Genetics of disease, diagnosis and treatmen