Effects of the interleukin-6 (IL-6) polymorphism on toxic metal and trace element levels in placental tissues

WOS: 000296128700005PubMed ID: 21911244The placenta is a crucial organ of fetal origin that functions in providing nutrients to the fetus from the mother. During pregnancy, the need for essential micronutrients, such as Fe and Zn, increases due to the requirements of the growing fetus. Maternal Fe deficiency induces an increase in Cu levels and can also affect cytokine levels in the placenta. On the other hand, Cu deficiency, although not as common, can also have destructive effects on the fetus. Interleukin-6 (IL-6) is a pleiotropic cytokine with a wide range of biological activities, including such as immune responses, acute-phase reactions, and inflammation. The placenta produces a significant amount of IL-6 during pregnancy. The effects of the IL-6 -174 G/C single nucleotide polymorphism (SNP) on IL-6 gene transcription and on plasma cytokine levels were assessed in the present study. We investigated the association between the IL-6 -174 G/C polymorphism and trace element/toxic metal levels in placental tissues. For the purposes of this study, 95 healthy volunteers were evaluated. Presence of the IL-6 polymorphism was determined using the standard polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) technique, and metal levels were analyzed by atomic absorption spectrometry (AAS). Based on our data, there were no significant associations between the IL-6 -174 G/C polymorphism and Pb, Cd, Fe, or Zn levels in the placental tissues (p > 0.05), but a statistically significant association was detected between the polymorphism and Cu levels (p = 0.016). We determined that the mean Cu levels in the placental tissues from individuals with CC, GC and CC genotypes were 5.62 +/- 1.98, 6.22 +/- 3.22 and 8.00 +/- 1.32 ppm, respectively, whereas the overall mean Cu level from the placental tissues was 5.98 +/- 2.51 ppm. (C) 2011 Elsevier B.V. All rights reserved

Simultaneous Headspace-GC-FID Analysis for Methanol and Ethanol in Blood, Saliva and Urine: Validation of Method and Comparison of Specimens

WOS: 000292808700001This study presents a headspace gas chromatography method with flame ionization detection (GC-FID) for the determination of ethanol and methanol content in biological fluids such as blood, urine and saliva, emphasizing matrix effects onto headspace analytical results. In this context, a simultaneous procedure was established and validated, and it was revealed that headspace GC for methanol and ethanol determination in all routinely available biological fluids is applicable as a reference method and also in routine diagnostics and monitoring. GC was performed with a run time of 6 min. The method showed linearity in the range of 50-400 mg/dL both for methanol and ethanol (r(2) = 0.998 and r(2) = 0.999, respectively) in all specimens with a detection and quantification limits less than 1.5 mg/dL and 2 mg/dL, respectively, as well as with good repeatability. Relative standard deviation (RSD) and percent bias were calculated for interday and intraday accuracy and precision at three concentrations (low 50 mg/dL, medium 200 mg/dL and high 400 mg/dL) for each biological fluid. A recovery rate of over 90% was obtained. Due to matrix effects of specimens, slight variations in validation data were presented and compared. The method and the obtained results suggest that the use of headspace GC could be extended from confirmatory analyses to routine application in toxicology laboratories.Ankara University Institute of BiotechnologyAnkara University [2004-118]This study was supported by grants from the Ankara University Institute of Biotechnology Research Fund, project number 2004-118

Simultaneous Headspace-GC-FID Analysis for Methanol and Ethanol in Blood, Saliva, and Urine: Validation of Method and Comparison of Specimens

WOS: 000280249200005This study presents a headspace gas chromatography method with flame ionization detection (GC-FID) for the determination of ethanol and methanol content in biological fluids such as blood, urine, and saliva, emphasizing matrix effects onto headspace analytical results. In this context, a simultaneous procedure was established and validated, and it was revealed that headspace GC for methanol and ethanol determination in all routinely available biological fluids is applicable as a reference method and also in routine diagnostics and monitoring. GC was performed with a run time of 6 min. The method showed linearity in the range of 50-400 mg/dL both for methanol and ethanol (r(2) = 0.998 and r(2) = 0.999, respectively) in all specimens with a detection and quantification limits less than 1.5 mg/dL and 2 mg/dL, respectively, as well as with good repeatability. Relative standard deviation and percent bias were calculated for interday and intraday accuracy and precision at three concentrations (low 50 mg/dL, medium 200 mg/dL, and high 400 mg/dL) for each biological fluid. A recovery rate of over 90% was obtained. Due to matrix effects of specimens, slight variations in validation data were presented and compared. The method and the obtained results suggest that the use of headspace GC could be extended from confirmatory analyses to routine application in toxicology laboratories.'Ankara University Institute of Biotechnology Research FundAnkara University [2004-118]This study was supported by grants from the Ankara University Institute of Biotechnology Research Fund, project number 2004-118

Abstracts of the First Eurasian Conference; The Coronavirus Pandemic and Critical ICT Infrastructure

While the world is struggling with COVID-19, the ICT industry seeks to play a constructive role in combating the spread of the virus. This book contains the abstracts of the papers presented at The First Eurasian Conference; The Coronavirus Pandemic and Critical ICT Infrastructure (PANDEMIC-ICT-2020) organized by AMIR Technical Services LLC, Tbilisi, Georgia held on November 28-30, 2020. Conference Title: The Coronavirus Pandemic and Critical ICT InfrastructureConference Acronym: PANDEMIC-ICT-2020Conference Date: 28-30 November 2020Conference Location: Online (Virtual Mode)Conference Organizer: AMIR Technical Services LLC, Tbilisi, Georgi

Abstracts of The Second Eurasian RISK-2020 Conference and Symposium

This abstract book contains abstracts of the various research ideas presented at The Second Eurasian RISK-2020 Conference and Symposium.The RISK-2020 Conference and Symposium served as a perfect venue for practitioners, engineers, researchers, scientists, managers and decision-makers from all over the world to exchange ideas and technology about the latest innovation developments dealing with risk minimization