Researches and grounds of design of pharmaceutical development and industrial introduction of production of parenteral preparations on the basis of recombinant proteins

In the conditions of introduction of the proper practice of production of medicinal facilities on pharmaceutical enterprises in Ukraine, it is necessary to develop effective measures of projects management for creation of favourable conditions for modernisation of pharmaceutical production, innovative management and management of development projects. The optimization of pharmaceutical production of injection preparations, modernisation of the pharmaceutical industries, is being implemented in accordance with the standards of GM

Molecular pedomorphism underlies craniofacial skeletal evolution in Antarctic notothenioid fishes

Background Pedomorphism is the retention of ancestrally juvenile traits by adults in a descendant taxon. Despite its importance for evolutionary change, there are few examples of a molecular basis for this phenomenon. Notothenioids represent one of the best described species flocks among marine fishes, but their diversity is currently threatened by the rapidly changing Antarctic climate. Notothenioid evolutionary history is characterized by parallel radiations from a benthic ancestor to pelagic predators, which was accompanied by the appearance of several pedomorphic traits, including the reduction of skeletal mineralization that resulted in increased buoyancy. Results We compared craniofacial skeletal development in two pelagic notothenioids, Chaenocephalus aceratus and Pleuragramma antarcticum, to that in a benthic species, Notothenia coriiceps, and two outgroups, the threespine stickleback and the zebrafish. Relative to these other species, pelagic notothenioids exhibited a delay in pharyngeal bone development, which was associated with discrete heterochronic shifts in skeletal gene expression that were consistent with persistence of the chondrogenic program and a delay in the osteogenic program during larval development. Morphological analysis also revealed a bias toward the development of anterior and ventral elements of the notothenioid pharyngeal skeleton relative to dorsal and posterior elements. Conclusions Our data support the hypothesis that early shifts in the relative timing of craniofacial skeletal gene expression may have had a significant impact on the adaptive radiation of Antarctic notothenioids into pelagic habitats

ANTIMICROBIAL ACTIVITY OF PROSTANORM® AGAINST THE UROPATHOGENS ISOLATED FROM PATIENTS WITH CHRONIC PROSTATITIS

Background. ProstaNorm® (0.2 g tablets and liquid extract for oral administration) is a complex medication (dry and liquid) extracted from a mixture of medicinal plants (Hyperici herb, Canadian goldenrod herb, Glycyrrhiza roots, Echinacea purpurea rhizome and roots). It has been successfully used during the last 20 years for the treatment of chronic prostatitis (CP) (especially the abacterial form), CP-associated infertility (spermatopathy), and for the restoration of normal urination. Previous studies have already demonstrated good antimicrobial activity of ProstaNorm®, which enables its use in the treatment of chronic bacterial prostatitis (CBP), especially taking into account other well-known effects of the drug (anti-inflammatory and analgesic activity, improvement of the prostatic tissue microcirculation and sperm characteristics).Objective: to evaluate in vitro bacteriostatic activity of ProstaNorm® against uropathogens isolated from patients with urinary tract infections (mostly with CP).Materials and methods. The following strains of gram-positive and gram-negative bacteria and fungi were isolated from urine, prostatic fluid, and ejaculate of patients with urinary tract infections: Streptococcus haemolyticus (n = 10), Streptococcus aureus (n = 3), D-group Streptococcus (n = 5), Enterococcus faecalis (n = 4), Enterococcus faecium (n = 5), Escherichia coli (n = 4), Klebsiella pneumonia (n = 3), Pseudomonas aeruginosa (n = 7), Candida albicans (n = 4), Candida glabrata (n = 2). We assessed antibacterial activity of ProstaNorm® (using the dilutions from 1:20 to 1:1280 of the initial 10% solution) against the isolated uropathogens.Results. We found that ProstaNorm® has a pronounced bacteriostatic effect against gram-negative bacteria (with the highest sensitivity shown by all Klebsiella pneumonia strains and 1 strain of Pseudomonas aeruginosa, when the microbial growth delay was 4.5–5.0 hours) and gram-positive bacteria (enterococci, hemolytic streptococci, and D-group streptococci). Additionally, the drug exhibits a bactericidal effect against some strains of gram-positive bacteria (three strains of streptococci). However, ProstaNorm® has no effect against Candida.Conclusion. Our results allow recommending ProstaNorm® for the treatment of chronic prostatitis accompanied by urinary tract infections

Researches and grounds of design of pharmaceutical development and industrial introduction of production of parenteral preparations on the basis of recombinant proteins

In the conditions of introduction of the proper practice of production of medicinal facilities on pharmaceutical enterprises in Ukraine, it is necessary to develop effective measures of projects management for creation of favourable conditions for modernisation of pharmaceutical production, innovative management and management of development projects. The optimization of pharmaceutical production of injection preparations, modernisation of the pharmaceutical industries, is being implemented in accordance with the standards of GM

Detergent-free solubilization of human Kv channels expressed in mammalian cells

International audienceStyrene-maleic acid (SMA) copolymers are used to extract lipid-encased membrane proteins from lipid bilayers in a detergent-free manner, yielding SMA lipid particles (SMALPs). SMALPs can serve as stable water-soluble nanocontainers for structural and functional studies of membrane proteins. Here, we used SMA copolymers to study full-length pore-forming α-subunits hKCNH5 and hKCNQ1 of human neuronal and cardiac voltage-gated potassium (Kv) channels, as well as the fusion construct comprising of an α-subunit hKCNQ1 and its regulatory transmembrane KCNE1 β-subunit (hKCNE1-hKCNQ1) with added affinity tags, expressed in mammalian COS-1 cells. All these recombinant proteins were shown to be functionally active. Treatment with the SMA copolymer, followed by purification on the affinity column, enabled extraction of all three channels. A DLS experiment demonstrated that Negative stain electron microscopy and single particle image analysis revealed a four-fold symmetry within channel-containing SMALPs, which indicates that purified hKCNH5 and hKCNQ1 channels, as well as the hKCNE1-hKCNQ1 fusion construct, retained their structural integrity as tetramers