Single Microbubble Imaging

The imaging of smaller vessels and organ perfusion has high clinical value for the di¬agnosis of cardiovascular disorders and cancer. Ultrasound imaging is the most used medical image modality since it is relatively cheap and flexible. Ultrasound contrast agents (UCAs) are used to enhance the signals from blood, thus facilitating the diagnosis. Such agents consist of stabilized gaseous microbubbles that are as small as red blood cells; their safety is widely acknowledged. This PhD thesis studies single microbubbles in experimental laboratory setups to investigate the behavior in body-like settings. Microbubbles are sonically excited at diagnostic frequencies in the range 1 – 10 MHz. We used the unique, custom-built camera Brandaris-128 (www.brandaris128.nl) to optically record the behavior at framing rates up to 25 million frames per second. Furthermore the echo-response from microbubbles is sampled with a dedicated acoustic setup. Both the optical and acoustic results in this thesis

The implications of K-Ar glauconite dating of the Diest Formation on the paleogeography of the Upper Miocene in Belgium

The glauconite-rich Diest Formation in central and north Belgium contains sands in the Campine subsurface and the hilly Hageland area that can be distinguished from each other. The Hageland Diest Sands member contains no stratigraphically relevant fossils while in the Campine subsurface dinoflagellate cysts are common and show a stratigraphic range covering the entire Tortonian stage. K-Ar dates were determined for glauconite from 13 selected samples spread over both areas. A glauconite date corresponding to the earliest Tortonian indicates newly formed glauconite was incorporated into a greensand at the base of the Diest Formation in the central Campine area. All other dates point at reworked glauconite and can be organized in two groups, one reflecting a Burdigalian age and another reflecting a Langhian age. These data and the thickness and glauconite content of the Diest Formation imply massive reworking of older Miocene deposits. The paleogeographic implications of these data lead to the tentative recognition of two Tortonian sedimentary sequences. An older one corresponding to dinoflagellate biochron DN8 comprises the Deurne Member, part of the Dessel Member, the Hageland Diest member, the eastern Campine Diest member and some basal sands of the Diest Formation in the central Campine. A younger sequence corresponding to dinoilagellate biochrons DN9 and 10 was strongly influenced by the prograding proto-Rhine delta front in the Roer Valley Graben to the northeast. The subsiding Campine basin was filled from east to west during this second cycle

Contaminant-induced immunotoxicity in harbour seals: Wildlife at risk?

Persistent, lipophilic polyhalogenated aromatic hydrocarbons (PHAHs) accumulate readily in the aquatic food chain and are found in high concentrations in seals and other marine mammals. Recent mass mortalities among several marine mammal populations have been attributed to infection by morbilliviruses, but a contributing role for immunotoxic PHAHs, including the polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins (PCDDs), and polychlorinated dibenzofurans (PCDFs) was not ruled out. We addressed this issue by carrying out a semi-field study in which captive harbour seals were fed herring from either the relatively uncontaminated Atlantic Ocean or the contaminated Baltic Sea for 2 years. We present here an overview of results obtained during this study. An impairment of natural killer (NK) cell activity, in vitro T-lymphocyte function, antigen-specific in vitro lymphocyte proliferative responses, and in vivo delayed-type hypersensitivity and antibody responses to ovalbumin was observed in the seals fed the contaminated Baltic herring. Additional feeding studies in PVG rats using the same herring batches suggested that an effect at the level of the thymus may be responsible for changes in cellular immunity, that virus-specific immune responses may be impaired, and that perinatal exposure to environmental contaminants represents a greater immunotoxic threat than exposure as a juvenile or adult. Together with the pattern of TCDD toxic equivalents of different PHAHs in the herring, these data indicate that present levels of PCBs in the aquatic food chain a

Morbillivirus infections of aquatic mammals: newly identified members of the genus.

Several disease outbreaks, which have caused the deaths of many thousands of seals and dolphins during the last decade, have now been attributed to infections with newly identified Morbilliviruses. Outbreaks in the late eighties amongst harbour seals (Phoca vitulina) and grey seals (Halichoerus grypus) in northwestern Europe and amongst baikal seals (Phoca sibirica) in Siberia were caused by the newly discovered phocine distemper virus and by a strain of canine distemper virus, respectively. Although closely related these two viruses were not identical. They were more distantly related to the viruses which caused mass mortality amongst striped dolphins (Stenella coeruleoalba) in the Mediterranean sea in the early nineties. This dolphin morbillivirus was shown to be closely related to a virus that was found in harbour porpoises (Phocoena phocoena) which had stranded at the coasts of northwestern Europe in the late eighties: porpoise morbillivirus. The present knowledge of the genetic and antigenic relationships of these apparently new members of the genus Morbillivirus with the established members of the genus is presented. In addition, the origin and epizootiological aspects of these newly discovered viruses are discussed. Finally experimental evidence that environmental pollution may have contributed to the severity and extent of these infections in recent years is presented

High-Frame-Rate Power Doppler Ultrasound Is More Sensitive than Conventional Power Doppler in Detecting Rheumatic Vascularisation

Early recognition of joint inflammation will increase treatment efficacy in rheumatoid arthritis (RA). Yet, conventional power Doppler (PD) ultrasound might not be sufficiently sensitive to detect minor inflammation. We investigated the sensitivity of high-frame rate Doppler, combined with singular value decomposition technique, to suppress tissue signals, for microvascular flow in a flow phantom setup and in a proof-of-principle study in healthy controls and in RA patients with different disease activities. In the flow phantom, minimal detectable flow velocity was a factor 3 lower with high-frame-rate PD than with conventional PD ultrasound. In the proof-of-principle study we detected a positive PD signal in all volunteers, diseased or healthy, with high-frame-rate PD ultrasound. We saw a gradual increase in PD signal in RA patients depending on disease activity. In conclusion, high-frame rate Doppler is more sensitive in detecting vascularisation than conventional PD ultrasound