THE MOLECULAR-GENETIC ANALYSIS OF POLYMORPHISM OF IL-4, TNF-α GENES IN PATIENTS WITH LIVER CIRROSIS AND PORTAL HYPERTENSION

Objective: to assess the association of polymorphism of the markers of interleukin-4 (IL-4) and tumor necrosis factor (TNF-α) in patients with liver cirrhosis with severe pathological process. Material and methods. The research material was DNA isolated from blood leukocytes of the patients. To identify SNP data, the PCR method was chosen with subsequent restriction analysis and electrophoretic detection by horizontal gel electrophoresis and amplification with visualization of the obtained results. Results. The performed molecular and genetic analysis of IL-4 and TNF-α gene polymorphisms made it possible to assume a relation between the frequency of polymorphic genotypes and alleles of the investigated genes and the severity of liver cirrhosis and portal hypertension, which can be used to diagnose and predict the severe course of the disease.</jats:p

The effect of the support nature and palladium dispersion on activity and selectivity of the palladium catalyst in hydrogenation of sunflower oil

Samples of the powdered catalysts Pd/C and Pd/Ox, which were synthesized by depositing 0.5 and 1.0 wt.% Pd on carbon materials (Sibunit 159k, thermal black Т-900, Vulcan XC-72R) and oxide supports (Ox: γ-Al2O3, Cr2O3, Ga2O3, TiO2, Ta2O5 and V2O5, and kieselguhr FW-70), were studied. Their catalytic properties were investigated in the partial hydrogenation of sunflower oil. Comparative testing of the catalysts was performed in the kinetic mode using a static Parr reactor to estimate their activity and trans-isomerization selectivity. The effect of the average size of supported palladium particles (dCO) on the indicated characteristics in a wide range of dCO values was discussed.</jats:p

Evolution of an isolated region in a stratified fluid

This paper reports an experimental study on the evolution of an isolated turbulent region in an otherwise quiescent linearly stratified fluid. A turbulent patch was generated by pulsed horizontal injection of a small volume of fluid. It was found that the turbulent blob thus produced initially grows as in a nonstratified fluid for a nondimensional time period of Nt ˜ 4 – 5 (with N the buoyancy frequency), attains a maximum height, and then physically collapses slowly to form a planar, quasi-two-dimensional dipole pattern. The timescale for the physical collapse of the blob was found to be much larger than that of the turbulence collapse. Scaling arguments are presented to explain the evolution of the turbulent region, and the experimental results are compared with the model predictions